1887

Abstract

Summary

The middle-size (M) genomic RNA of a New York State, U.S.A. isolate of La Crosse (LAC) virus has been cloned by a random priming procedure and its nucleotide sequence determined by the dideoxy method. The RNA was found to be 4526 nucleotides in length and to have a base composition of 34·2% U, 27·8% A, 20·6% C and 17·4% G. There is a single, long open reading frame in the viral complementary RNA that contains sufficient information to code for a protein of 1441 amino acids. In these respects, as in many others, the LAC virus M RNA and its encoded protein were very similar, if not identical, to those previously reported by other investigators for the closely related snowshoe hare virus. The M RNAs of the two viruses show 79% nucleotide sequence homology and 89% homology in the amino acid sequence of their encoded proteins. Several algorithms for predicting surface residues, as well as the Chou-Fasman rules for predicting secondary structure, were used to compare the LAC virus and snowshoe hare virus M gene proteins. These analyses identified 39 sites on the proteins as those most likely to be linear antigenic determinants that might contribute to the differences between the two viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-12-3057
1987-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/12/JV0680123057.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-12-3057&mimeType=html&fmt=ahah

References

  1. Akashi H., Bishop D. H. L. 1983; Comparison of the sequence and coding of La Crosse and snowshoe hare bunyavirus S RNA species. Journal of Virology 45:1155–1158
    [Google Scholar]
  2. Akashi H., Gay M., Ihara T., Bishop D. H. L. 1984; Localized conserved regions of the S RNA gene products of bunyaviruses are revealed by sequence analysis of the Simbu serogroup Aino virus. Virus Research i:51–63
    [Google Scholar]
  3. Beaty B. J., Holterman M., Tabachnick W., Shope R. E., Rozhon E. J., Bishop D. H. L. 1981a; Molecular basis of bunyavirus transmission by mosquitoes: role of the middle-sized RNA segment. Science211–1433
    [Google Scholar]
  4. Beaty B. J., Miller B. R., Holterman M., Tabachnick W., Shope R. E., Rozhon E. J., Bishop D. H. L. 1981b; Pathogenesis of bunyavirus reassortants in Aedes triseriatusmosquitoes. In The Replication of Negative Strand Viruses pp 153–158 Bishop D. H. L., Compans R. W. Edited by New York: Elsevier/North-Holland;
    [Google Scholar]
  5. Binns M. M., Boursnell M. E. g., Foulds I. J., Brown T. D. K. 1985; The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus. Journal ofVirological Methods 11:265–269
    [Google Scholar]
  6. Bishop D. H. L., Shope R. E. 1979; Bunyaviridae. In Comprehensive Virology 14 pp 1–156 Fraenkel-Conrat H., Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  7. Cabradilla C. D.Jr Holloway B. P., Obijeski J. F. 1983; Molecular cloning and sequencing of the La Crosse virus S RNA. Virology 128:463–468
    [Google Scholar]
  8. Calisher C. H. 1983; Taxonomy, classification, and geographic distribution of California serogroup bunyaviruses. In California Serogroup Viruses pp 1–16 Calisher C. H., Thompson W. H. Edited by New York: Alan R. Liss;
    [Google Scholar]
  9. Cash P., Vezza A. C., Gentsch J. R., Bishop D. H. L. 1979; Genome complexities of the three mRNA species of snowshoe hare bunyavirus and in vitrotranslation of S mRNA to viral N polypeptide. Journal of Virology 31:685–694
    [Google Scholar]
  10. Cohen S. n., Chang A. C. Y., Hsu C. L. 1972; Non-chromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coliby R factor DNA. Proceedings of the National Academy of Sciences U.S.A.: 692110–2114
    [Google Scholar]
  11. Chou P. Y., Fasman G. D. 1978; Prediction of the secondary structure of proteins from their amino acid sequence. Advances in Enzymology 47:45–148
    [Google Scholar]
  12. Clerx-Van Haaster C. M., Akashi H., Auperin D. D., Bishop D. H. L. 1982; Nucleotide sequence analysis and predicted coding of bunyavirus genome RNA species. Journal of Virology 41:119–128
    [Google Scholar]
  13. Collett M. S., Purchio A. F., Keegan K., Frazier S., Hays W., Anderson D. K., Parker M. D., Schmaljohn C., Schmidt J., Dalrymple J. M. 1985; Complete nucleotide sequence of the M RNA segment of Rift Valley fever virus. Virology 144:228–245
    [Google Scholar]
  14. Conrad B., Mount D. W. 1982; Microcomputer programs for DNA sequence analysis. Nucleic Acids Research 10:31–37
    [Google Scholar]
  15. Corrigan A. J., Huang P. C. 1982; A BASIC microcomputer program for plotting the secondary structure of proteins. Computer Programs in Biomedicine 15:163–168
    [Google Scholar]
  16. Eshita Y., Bishop D. H. L. 1984; The complete sequence of the M RNA of snowshoe hare bunyavirus reveals the presence of internal hydrophobic domains in the viral glycoprotein. Virology 137:227–240
    [Google Scholar]
  17. Fasman G. D. 1985; Prediction of protein structure from sequence. Nature; London: 31622
    [Google Scholar]
  18. Fuller F., Bishop D. H. L. 1982; Identification of viral coded nonstructural polypeptides in bunyavirus infected cells. Journal of Virology 41:643–648
    [Google Scholar]
  19. Fuller F., Brown A. S., Bishop D. H. L. 1983; Bunyavirus nucleoprotein, N, and a non-structural protein NSs, are coded by overlapping reading frames in the S RNA. Journal of General Virology 64:1705–1714
    [Google Scholar]
  20. Gentsch J. R., Bishop D. H. L. 1978; Small viral RNA segment of bunyaviruses codes for viral nucleocapsid protein. Journal of Virology 28:417–419
    [Google Scholar]
  21. Gentsch J. R., Bishop D. H. L. 1979; The M viral RNA segment of bunyaviruses codes for two unique glycoproteins: G1 and G2. Journal of Virology 30:767–770
    [Google Scholar]
  22. Gonzalez-Scarano F., Shope R. E., Calisher C. H., Nathanson N. 1982; Characterization of monoclonal antibodies against the G1 and N proteins of La Crosse and Tahyna, two California serogroup bunyaviruses. Virology 120:42–53
    [Google Scholar]
  23. Gonzalez-Scarano F., Pobjecky N., Nathanson N. 1984; La Crosse bunyavirus can mediate pH-dependent fusion from without. Virology 132:222–225
    [Google Scholar]
  24. Gonzalez-Scarano F., Janssen R. S., Najjar J. A., Pobjecky N., Nathanson N. 1985; An avirulent G1 glycoprotein variant of La Crosse bunyavirus with defective fusion function. Journal of Virology 54:757–763
    [Google Scholar]
  25. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  26. Hopp T. P., Woods K. R. 1981; Prediction of protein antigenic determinants from amino acid sequences. Proceedings of the National Academy of Sciences U.S.A.:78–3824
    [Google Scholar]
  27. Grady L. J., Kinch W. 1985; Two monoclonal antibodies against La Crosse virus show host-dependent neutralizing activity. Journal of General Virology 66:2773–2776
    [Google Scholar]
  28. Grady L. J., Srihongse S., Grayson M. A., Deibel R. 1983; Monoclonal antibodies against La Crosse virus. Journal of General Virology 64:1699–1704
    [Google Scholar]
  29. Graham A., Steven I., Mckechnie D., Harris W. J. 1986; Direct DNA sequencing using avian myeloblastosis virus and Moloney-murine leukemia virus reverse transcriptase. Focus 8:4–5
    [Google Scholar]
  30. Ihara T., Smith J., Dalrymple J. M., Bishop D. H. L. 1985; Complete sequence of the glycoproteins and M RNA of Punta Toro Phlebovirus compared to those of Rift Valley fever virus. Virology 144:246–259
    [Google Scholar]
  31. Imaizumi T., Diggelmann H., Scherrer K. 1973; Demonstration of globin messenger sequences in giant nuclear precursors of messenger RNA of avian erythroblasts. Proceedings of the National Academy of Sciences U.S.A.: 701122–1126
    [Google Scholar]
  32. Janin J. 1979; Surface and inside volumes in globular proteins. Nature: London; 277491–492
    [Google Scholar]
  33. Janssen R. S., Nathanson N., Endres M. J., Gonzalez-Scarano F. 1986; Virulence of La Crosse virus is under polygenic control. Journal of Virology 59:1–7
    [Google Scholar]
  34. Kabsch W., Sander C. 1983; How good are predictions of protein secondary structure?. FEBS tetters 155:179–182
    [Google Scholar]
  35. Karplus P. A., Schulz G. E. 1985; Prediction of chain flexibility in proteins. Naturwissenschaften 72:212–213
    [Google Scholar]
  36. Kingsford L., Hill D. W. 1981; The effects of proteolytic enzymes on structure and function of La Crosse G1 and G2 glycoproteins. In The Replication of Negative Strand Viruses pp 111–116 Bishop D. H. L., Compans R. W. Edited by New York: Elsevier/North-Holland;
    [Google Scholar]
  37. Kingsford L., Ishizawa L. D. 1984; Antigenic sites on the G1 glycoprotein of La Crosse virus that are involved in neutralization. In Segmented Negative Strand Viruses pp 217–224 Compans R. W., Bishop D. H. L. Edited by Orlando: Academic Press;
    [Google Scholar]
  38. Kingsford L., Ishizawa L. D., Hill D. W. 1983; Biological activities of monoclonal antibodies reactive with antigenic sites mapped on the G1 glycoprotein of La Crosse virus. Virology 129:443–455
    [Google Scholar]
  39. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  40. Lees J. F., Pringle C. R., Elliott R. M. 1986; Nucleotide sequence of the Bunyamwera virus M RNA segment: conservation of structural features in the bunyavirus glycoprotein gene product. Virology 148:1–14
    [Google Scholar]
  41. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  42. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  43. Maxam A. M., Gilbert W. 1977; A new method for sequencing DNA. Proceedings of the National Academy of Sciences U.S.A.: 74560–564
    [Google Scholar]
  44. Messing J. 1979; A multipurpose cloning system based on the single-stranded DNA bacteriophage M13.. Recombinant DNA Technical Bulletin, NIH Publication79–992–43
    [Google Scholar]
  45. Novotny I., Handschumacher M., Bruccoleri R. E. 1987; Protein antigenicity: a static surface property. Immunology Today 8:26–31
    [Google Scholar]
  46. Obijeski J. F., Bishop D. H. L., Murphy F. A., Palmer E. R. 1976a; Structural proteins of La Crosse virus. Journal of Virology19–985
    [Google Scholar]
  47. Obijeski J. F., Bishop D. H. L., Palmer E. L., Murphy F. A. 1976b; Segmented genome and nucleocapsid of La Crosse virus. Journal of Virology 20:664–675
    [Google Scholar]
  48. Parker J. M. R., Guo D., Hodges R. S. 1986; New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites. Biochemistry 25:5425–5432
    [Google Scholar]
  49. Pellett P. E., Kousoulas K. G., Pereira L., Roizman B. 1985; Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants. Journal of Virology 53:243–253
    [Google Scholar]
  50. Perlman D., Halvorson H. O. 1983; A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. Journal of Molecular Biology 167:391–409
    [Google Scholar]
  51. Rose G. D., Gierasch L. M., Smith J. A. 1985; Turns in peptides and proteins. In Advances in Protein Chemistry 37 pp 1–109 Anfinsen C. B., Edsall J. T., Richards F. M. Edited by Orlando:
    [Google Scholar]
  52. Rozhon E. L., Gensemer P., Shope R. E., Bishop D. H. L. 1981; Attenuation of virulence of a bunyavirus involving an L RNA defect and isolation of LAC/SSH/LAC and LAC/SSH/SSH reassortants. Virology 111:125–138
    [Google Scholar]
  53. Ruther U., Koenen M., Otto K., Muller-Hill B. 1981; pUR222, a vector for cloning and rapid chemical sequencing of DNA. Nucleic Acids Research 9:4087–4098
    [Google Scholar]
  54. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A.: 745463–5467
    [Google Scholar]
  55. Schmaljohn C. S., Schmaljohn A. L., Dalrymple J. M. 1987; Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order. Virology 157:31–39
    [Google Scholar]
  56. Shope R. E., Rozhon E. J., Bishop D. H. L. 1981a; Role of the middle-sized bunyavirus RNA segment in mouse virulence. Virology 114:273–276
    [Google Scholar]
  57. Shope R. E., Tignor G. H., Rozhon E. J., Bishop D. H. L. 1981b; The association of the bunyavirus middle-sized RNA segment with mouse pathogenicity. In The Replication of Negative Strand Viruses pp 147–152 Bishop D. H. L., Compans R. W. Edited by New York: Elsevier/North-Holland;
    [Google Scholar]
  58. Sundin D. R., Beaty B. J., Nathanson N., Gonzalez-Scarano F. 1987; A G1 glycoprotein epitope of La Crosse virus: a determinant of infection of Aedes triseriatus. Science 235:591–593
    [Google Scholar]
  59. Tignor G. H., Burrage T. G., Smith A. L., Shope R. E., Bishop D. H. L. 1983; California serogroup gene structure-function relationships: virulence and tissue tropisms. In California Serogroup Viruses pp 129–138 Calisher C. H., Thompson W. H. Edited by New York: Alan R. Liss;
    [Google Scholar]
  60. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  61. Westhof E., Altschuh D., Moras D., Bloomer A. C., Mondragon A., Klug A., Van Regenmortel M. H. V. 1984; Correlation between segmental mobility and the location of antigenic determinants in proteins. Nature: London; 311123–126
    [Google Scholar]
  62. Wunner W. H., Dietzschold B., Smith C. L., Lafon M., Golub W. 1985; Antigenic variants of CVS rabies virus with altered glycosylation sites. Virology 140:1–12
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-12-3057
Loading
/content/journal/jgv/10.1099/0022-1317-68-12-3057
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error