1887

Abstract

Summary

We have developed a selection protocol to isolate interferon (IFN)-sensitive subclones directly from an IFN-resistant cell population. The protocol uses encephalo- myocarditis virus (EMCV) as a selection agent in combination with pretreatment with low doses of IFN and subsequent growth in the presence of virus-neutralizing antiserum. We have applied this protocol to the partially IFN-resistant NIH 3T3 clone 1 line and have obtained a number of IFN-sensitive subclones. Sensitivity to IFN was restricted to protection against EMCV. Replication of vesicular stomatitis virus as well as cell growth were resistant to IFN treatment as in the original clone 1 line. We have compared levels of 2′,5′-oligoadenylate (2–5A) synthetase, dsRNA-activated protein kinase and 2–5A-dependent RNase in some IFN-sensitive subclones and found no difference from the resistant clone 1 cells. Markedly decreased levels of 2–5A- dependent RNase and thus a defective 2–5A pathway have been implicated as a possible cause for the partial resistance of clone 1 cells to IFN. Since the selected IFN- sensitive subclones are of the same phenotype in this respect as the clone 1 line we suggest that inhibition of EMCV in these lines occurs through a mechanism independent of the 2–5A system.

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1987-11-01
2024-12-03
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References

  1. Ankel H., Mittnacht S., Jacobsen H. 1985; Antiviral activity of prostaglandin A on encephalomyocarditis virus-infected cells: a unique effect unrelated to interferon. Journal of General Virology 66:2355–2364
    [Google Scholar]
  2. Baglioni C. 1979; Interferon-induced enzymatic activities and their role in the antiviral state. Cell 17:255–264
    [Google Scholar]
  3. Caley P. J., Knight M., Kerr I. M. 1982; Virus-mediated inhibition of the ppp(A2′p)nA system and its prevention by interferon. Biochemical and Biophysical Research Communications 104:376–381
    [Google Scholar]
  4. Czarniecki C. W., Sreevalsan T., Friedman R. M., Panet A. 1981; Dissociation of interferon effects on murine leukemia virus and encephalomyocarditis virus replication in mouse cells. Journal of Virology 37:827–831
    [Google Scholar]
  5. Epstein D. A., Czarniecki C. W., Jacobsen H., Friedman R. M., Panet A. 1981; A mouse cell line, which is unprotected by interferon against lytic virus infection, lacks ribonuclease F activity. European Journal of Biochemistry 118:9–15
    [Google Scholar]
  6. Friedman R. M. 1977; Antiviral activity of interferons. Bacteriological Reviews 41:543–567
    [Google Scholar]
  7. Gresser I., Bandu M.-T., Brouty-Boyé D. 1974; Interferon and cell division. IX. Interferon-resistant L1210 cells: characteristics and origin. Journal of the National Cancer Institute 52:553–559
    [Google Scholar]
  8. Gupta S. L., Holmes S. L., Mehra L. L. 1982; Interferon action against reovirus: activation of interferon-induced protein kinase in mouse L929 cells upon reovirus infection. Virology 120:495–499
    [Google Scholar]
  9. Horisberger M. A., Staeheli P., Haller O. 1983; Interferon induces a unique protein in cells bearing a gene for resistance to influenza virus. Proceedings of the National Academy of Sciences, U.S.A 80:1910–1914
    [Google Scholar]
  10. Hovanessian A. G., Kerr I. M. 1979; The (2′-5′)oligoadenylate (pppA2′ 5′A2′ 5′A) synthetase and protein kinase(s) from interferon-treated cells. European Journal of Biochemistry 93:515–526
    [Google Scholar]
  11. Hovanessian A. G., Wood J., Meurs E., Montagnier L. 1979; Increased nuclease activity in cells treated with pppA2′p5′A2′p5′A. Proceedings of the National Academy of Sciences, U.S.A 76:3261–3265
    [Google Scholar]
  12. Johnston M. I., Torrence P. F. 1984; The role of interferon-induced proteins, double-stranded RNA and 2′,5′-oligoadenylate in the interferon-mediated inhibition of viral translation. In Interferon 3 pp. 189–298 Friedman R. M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  13. Knight M., Cayley P. J., Silverman R. H., Wreschner D. H., Gilbert C. S., Brown R. E., Kerr I. M. 1980; Radioimmune, radiobinding and HPLC analysis of 2-5A and related oligonucleotides from intact cells. Nature; London: 288189–192
    [Google Scholar]
  14. Krause D., Panet A., Arad G., Dieffenbach C. W., Silverman R. H. 1985a; Independent regulation of ppp(A2′p)nA-dependent RNase in NIH 3T3, clone 1 cells by growth arrest and interferon treatment. Journal of Biological Chemistry 260:9501–9507
    [Google Scholar]
  15. Krause D., Silverman R. H., Jacobsen H., Leisy S. A., Dieffenbach C. W., Friedman R. M. 1985b; Regulation of ppp(A2′p)nA-dependent RNase levels during interferon treatment and cell differentiation. European Journal of Biochemistry 146:611–618
    [Google Scholar]
  16. Lengyel P. 1982; Biochemistry of interferons and their actions. Annual Review of Biochemistry 51:251–282
    [Google Scholar]
  17. Nilsen T. W., Wood D. L., Baglioni C. 1980; Virus-Specific effects of infection in embryonal carcinoma cells. Nature; London: 286178–180
    [Google Scholar]
  18. Nilsen T. W., Candless S., Baglioni C. 1982; 2′,5′-Oligo(A)-activated endonuclease in NIH 3T3 mouse cells chronically infected with Moloney murine leukemia virus. Virology 122:498–502
    [Google Scholar]
  19. Salzberg S., Wreschner D. H., Oberman F., Panet A., Bakkanashuili M. 1983; Isolation and characterization of an interferon resistant cell line deficient in the induction of (2′-5′) oligoadenylate synthetase activity. Molecular and Cellular Biology 3:1759–1765
    [Google Scholar]
  20. Samuel C. E., Knutson G. S. 1982a; Mechanism of interferon action. Kinetics of induction of the antiviral state and protein phosphorylation in mouse fibroblasts treated with natural and cloned interferons. Journal of Biological Chemistry 257:11791–11795
    [Google Scholar]
  21. Samuel C. E., Knutson G. S. 1982b; Mechanism of interferon action. Kinetics of decay of the antiviral state and protein phosphorylation in mouse fibroblasts treated with natural and cloned interferons. Journal of Biological Chemistry 257:11796–11801
    [Google Scholar]
  22. Silverman R. H., Wreschner D. H., Gilbert C. s., Kerr I. M. 1981; Synthesis, characterization and properties of ppp(A2′P)nApCp and related high-specific-activity 32P-labelled derivatives of ppp(A2′p)nA. European Journal of Biochemistry 115:79–85
    [Google Scholar]
  23. Staeheli P., Haller O., Boll W., Lindenmann J., Weissmann C. 1986; Mx protein: constitutive expression in 3T3 cells transformed with cloned MxcDNA confers selective resistance to influenza virus. Cell 44:147–158
    [Google Scholar]
  24. Watling D., Serfainowska H. T., Reese C. B., Kerr I. M. 1985; Analogue inhibitor of 2-5A action: effect of the interferon-mediated inhibition of encephalomyocarditis virus replication. EMBO Journal 4:431–436
    [Google Scholar]
  25. Williams B. R. G., Golgher R. R., Brown R. E., Gilbert C. s., Kerr I. M. 1979; Natural occurrence of 2-5A in interferon-treated EMC virus-infected L cells. Nature; London: 282582–586
    [Google Scholar]
  26. Wood J. N., Hovanessian A. G. 1979; Interferon enhances 2-5A synthetase in embryonal carcinoma cells. Nature; London: 28274–76
    [Google Scholar]
  27. Wreschner D. H., James T. C., Silverman R. H., Kerr I. M. 1981; Ribosomal RNA cleavage, nuclease activation and 2-5A (ppp(A2′P)nA) in interferon-treated cells. Nucleic Acids Research 9:1571–1581
    [Google Scholar]
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