1887

Abstract

Summary

In Rat-9G cells several copies of the major immediate early (IE) transcription unit (regions 1 and 2) of the human cytomegalovirus (HCMV) are stably integrated. The cells show a repressed phenotype for IE expression but can be induced by inhibition of protein synthesis. In this report we present evidence that the repressed phenotype is due to the absence of IE transcription and that heat-shock and sodium arsenite treatments each result in the transcriptional activation of the repressed IE transcription unit. Either treatment resulted in the induction of HCMV IE transcripts and IE nuclear antigen expression. An octameric DNA sequence present in three of the 18 bp IE enhancer elements (GGACTTTC) resembles the cellular heat-shock element core consensus sequence and may therefore be involved in the heat-shock response.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-11-2925
1987-11-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/11/JV0680112925.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-11-2925&mimeType=html&fmt=ahah

References

  1. Akrigg A., Wilkinson G. W. G., Oram J. D. 1985; The structure of the major immediate early gene of human cytomegalovirus. Virus Research 2:107–121
    [Google Scholar]
  2. Ashburner M., Bonner J. J. 1979; The induction of gene activity in Drosophila by heat-shock. Cell 17:241–254
    [Google Scholar]
  3. Blanton R. A., Tevethia M. J. 1981; Immunoprecipitation of virus specific immediate early and early polypeptides from cells lytically infected with human cytomegalovirus strain AD169. Virology 112:262–273
    [Google Scholar]
  4. Boom R., Geelen J. L., Sol C. J., Raap A. K., Minnaar R. P., Klaver B. P., Van Der Noordaa J. 1986; Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibition. Journal of Virology 58:851–859
    [Google Scholar]
  5. Boshart M., Weber F., Jahn G., Dorsch-Häsler K., Fleckenstein B., Schaffner W. 1985; A very Strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 41:521–530
    [Google Scholar]
  6. Burdon R. H. 1986; Heat shock and the heat shock proteins. Biochemical Journal 240:313–324
    [Google Scholar]
  7. Clemens M. J. 1984; Purification of eukaryotic messenger RNA. In Transcription and Translation pp. 211–270 Hames B. D., Higgins S. J. Edited by Oxford: IRL Press;
    [Google Scholar]
  8. Demarchi J. M. 1981; Human cytomegalovirus DNA: restriction enzyme cleavage maps and locations for the immediate early, early and late RNAs. Virology 114:23–28
    [Google Scholar]
  9. Goldstein L. C., Mcdougall J., Hackman R., Meyers J. D., Thomas E. D., Nowinski R. C. 1982; Monoclonal antibodies to cytomegalovirus: rapid identification of clinical isolates and preliminary use in diagnosis of cytomegalovirus pneumonia. Infection and Immunity 38:273–281
    [Google Scholar]
  10. Greenberg M. E., Ziff E. B. 1983; Stimulation of 3T3 cells induces transcription of c-fos proto-oncogene. Nature; London: 311433–438
    [Google Scholar]
  11. Imperiale M. J., Kao H. T., Feltman L. T., Nevins J. R., Strickland S. 1984; Common control of the heat shock gene and early adenovirus genes: evidence for a cellular E1 A-like activity. Molecular and Cellular Biology 4:867–874
    [Google Scholar]
  12. Jahn G., Knust E., Schmolla H., Sarre T., Nelson J. A., Mcdougall J. K., Fleckenstein B. 1984; Predominant immediate early transcripts of human cytomegalovirus ADI 69. Journal of Virology 49:363–370
    [Google Scholar]
  13. Jordan M. c. 1983; Latent infection and the elusive cytomegalovirus. Review of Infectious Diseases 5:205–215
    [Google Scholar]
  14. Mcdonough S. H., Spector D. H. 1983; Transcription in human fibroblasts permissively infected by human cytomegalovirus strain AD169. Virology 125:31–46
    [Google Scholar]
  15. Nevins J. R. 1982; Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus El Agene product. Cell 29:913–919
    [Google Scholar]
  16. Notarianni E. L., Preston C. M. 1982; Activation of cellular stress protein genes by herpes simplex virus temperature sensitive mutants which overproduce immediate early polypeptides. Virology 123:113–122
    [Google Scholar]
  17. Pelham H. 1985; Activation of heat-shock genes in eukaryotes. Trends in Genetics 1:31–35
    [Google Scholar]
  18. Schöffl F., Raschke E., Nagao R. T. 1984; The DNA sequence analysis of soybean heat-shock genes and identification of possible regulatory promoter elements. EMBO Journal 3:2491–2497
    [Google Scholar]
  19. Stenberg R. M., Witte P. R., Stinski M. F. 1985; Multiple spliced and unspliced transcripts from human cytomegalovirus immediate-early region 2 and evidence for a common initiation site within immediate-early region 1. Journal of Virology 56:665–675
    [Google Scholar]
  20. Stinski M. F., Roehr T. J. 1985; Activation of the major immediate early gene of human cytomegalovirus by exacting elements in the promoter-regulatory sequence and by virus-specific trans-acting components. Journal of Virology 55:431–441
    [Google Scholar]
  21. Stinski M. F., Thomson D. R., Stenberg R. M., Goldstein L. C. 1983; Organization and expression of the immediate early genes of human cytomegalovirus. Journal of Virology 46:1–14
    [Google Scholar]
  22. Topp W. C. 1981; Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113:408–411
    [Google Scholar]
  23. Wathen M. W., Stinski M. F. 1982; Temporal patterns of human cytomegalovirus transcription: mapping of the viral RNA synthesized at immediate early, early and late times after infection. Journal of Virology 41:462–477
    [Google Scholar]
  24. Wilkinson G. W. G., Akrigg A., Greenaway P. 1984; Transcription of the immediate early genes of human cytomegalovirus strain AD169. Virus Research 1:101–116
    [Google Scholar]
  25. Zerbini M., Musiani M., La Placa M. 1986; Stimulating effect of heat-shock on the early stage of human cytomegalovirus replication cycle. Virus Research 6:211–216
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-11-2925
Loading
/content/journal/jgv/10.1099/0022-1317-68-11-2925
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error