1887

Abstract

Summary

The structures at the 5′ termini of the two genomic RNA species of cherry leaf roll virus (CLRV) were found to be neither 7-methylguanosine caps, nor 5′-hydroxyl groups or 5′-phosphate groups. CLRV RNA could be iodinated using NaI and Iodogen; the labelled moiety appeared to be covalently linked to the RNA and could be removed by treatment with Pronase or proteinase K without altering the electrophoretic mobility of the RNA. The infectivity of RNA was greatly diminished by these enzymes, but such treatment impaired neither the fidelity nor the efficiency of translation of the RNA Electrophoresis of the acetone-precipitable material released from the RNA by ribonuclease A treatment yielded a single radioactive component corresponding in mobility to a protein with a mol. wt. of about 3500. This molecule (VPg) presumably corresponds to the protease-sensitive structure needed for infectivity. An antiserum prepared against RNA of the birch isolate of CLRV precipitated homologous VPg as well as those of the rhubarb, dogwood, walnut and isolates of CLRV, but not the VPgs of arabis mosaic nepovirus or tomato black ring nepovirus.

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1987-11-01
2024-12-14
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References

  1. Chu P. W. G., Boccardo G., Franckl R. I. B. 1981; Requirement of a genome-associated protein of tobacco ringspot virus for infectivity but not in vitro translation. Virology 109:428–430
    [Google Scholar]
  2. Cooper J. I., Atkinson M. A. 1975; Cherry leaf roll virus causing a disease of Betula spp. in the United Kingdom. Forestry 48:193–203
    [Google Scholar]
  3. Dasgupta R., Harada F., Kaesburg P. 1976; Blocked 5′ termini in brome mosaic virus RNA. Journal of Virology 18:260–267
    [Google Scholar]
  4. Donis-Keller H., Maxam A. M., Gilbert W. 1977; Mapping adenines, guanines and pyrimidines in RNA. Nucleic Acids Research 4:2527–2538
    [Google Scholar]
  5. Edwards M.-L., Cooper J. I., Massalski P. R. 1983; Some natural hosts of brome mosaic virus in the United Kingdom. Plant Pathology 32:91–94
    [Google Scholar]
  6. Harrison B. D., Barker H. 1978; Protease-sensitive structure needed for infectivity of nepovirus RNA. Journal of General Virology 40:711–715
    [Google Scholar]
  7. Harrison B. D., Nixon H. L. 1960; Purification and electron microscopy of three soil-borne plant viruses. Virology 12:104–117
    [Google Scholar]
  8. Hickey E. D., Weber L. A., Baglioni C. 1976; Inhibition of initiation of protein synthesis by 7-methylguanosine 5'-monophosphate. Proceedings of the National Academy of Sciences, U.S.A 73:19–23
    [Google Scholar]
  9. Jones A. T. 1985; Cherry leaf roll virus. Association of Applied Biologists Descriptions of Plant Viruses no. 306 (no. 80 revised)
    [Google Scholar]
  10. Koenig I., Fritsch C. 1982; A protein linked at the 5′ end of satellite and genomic tomato black ring virus RNAs: study of in vitro translation after protease treatment. Journal of General Virology 60:343–353
    [Google Scholar]
  11. Massalski P. R., Cooper J. I. 1986; Comparison of the genome RNA sequence homologies between isolates of cherry leaf roll virus by complementary DNA hybridization analysis. Journal of General Virology 67:1169–1172
    [Google Scholar]
  12. Mayo M. A., Barker H., Harrison B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  13. Pelham H. R. B. 1979; Translation of tobacco rattle virus RNAs in vitro; four proteins from three RNAs. Virology 97:256–265
    [Google Scholar]
  14. Salacinski P. R. P., Mclean C., Sykes J. E. C., Clement-Jones V. V., Lowry P. J. 1981; Iodination of proteins, glycoproteins and peptides using a solid-phase oxidising agent, 1,3,4,6-tetrachloro-3α,6α-diphenyl glycoluril (Iodogen). Analytical Biochemistry 117:136–146
    [Google Scholar]
  15. Stace-Smith R. 1966; Purification and properties of tomato ringspot virus and an RNA-deficient component. Virology 29:240–242
    [Google Scholar]
  16. Zabel P., Moerman M., Van Straaten F., Goldbach R., Van Kammen A. 1982; Antibodies against the genome-linked protein VPg of cowpea mosaic virus recognise a 60,000-dalton precursor polypeptide. Journal of Virology 41:1083–1088
    [Google Scholar]
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