1887

Abstract

Summary

Five of five human teratocarcinomas cultured could be induced to synthesize retrovirus-like particles, albeit in extremely low amounts. The accumulation and purification of the human teratocarcinoma-derived retrovirus (HTDV) from one of these cell lines allowed characterization of its genomic material as high mol. wt. (60S) RNA. Partial purification of HTDV-associated RNA-dependent DNA polymerase (reverse transcriptase) has also been achieved. HTDVs are easily distinguishable from the exogenous human T-lymphotropic viruses and human immunodeficiency viruses by morphological, biological and immunological criteria.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-11-2807
1987-11-01
2024-12-03
Loading full text...

Full text loading...

References

  1. Barbacid M., Bolognesi D. p., Aaronson S. A. 1980; Humans have antibodies capable of recognizing oncoviral glycoproteins: demonstration that these antibodies are formed in response to cellular modification of glycoproteins rather than as consequence of exposure to virus. Proceedings of the National Academy of Sciences, U.S.A 77:1617–1621
    [Google Scholar]
  2. Barré-Sinoussi F., Chermann Y. C., Rey F., Nugeyre M. T., Chamaret S., Guest I., Dauguet C., Axler-Blin C., Vezinet-Brun F., Rouzioux C., Rozenbaum W., Montagnier L. 1983; Isolation of a T-lymphotropic retrovirus from a patient at risk of acquired immune deficiency syndrome (AIDS). Science 220:868–871
    [Google Scholar]
  3. Boller K., Frank H., Löwer J., Löwer R., Kurth R. 1983; Structural organization of unique retrovirus-like particles budding from human teratocarcinoma cell lines. Journal of General Virology 64:2549–2559
    [Google Scholar]
  4. Dirksen E. R., Levy J. A. 1977; Virus-like particles in placentas from normal individuals and patients with systemic lupus erythematosus. Journal of the National Cancer Institute 59:1187–1192
    [Google Scholar]
  5. Fogh J., Trempe G. 1975; New human tumor cell lines. In Human Tumor Cells in vitro pp. 115–159 Fogh J. Edited by New York: Plenum Press;
    [Google Scholar]
  6. Frank H., Schwarz H., Graf T., Schäfer W. 1978; Properties of mouse leukemia viruses. XV. Electron microscopic studies on the organization of Friend leukemia virus and other mammalian C-type viruses. Zeitschrift für Naturforschung 33c:124–138
    [Google Scholar]
  7. Goldstein J. L., Anderson R. G. W., Brown M. S. 1979; Coated pits, coated vesicles and receptor-mediated endocytosis. Nature; London: 279679–685
    [Google Scholar]
  8. Hamilton M. G., Ruth M. E. 1969; The dissociation of rat liver ribosomes by ethylene diaminetetra acetic acid; molecular weights, chemical composition, and buoyant densities of the subunits. Biochemistry 8:851–856
    [Google Scholar]
  9. Harzmann R., Löwer J., Löwer R., Bichler K.-H., Kurth R. 1982; Synthesis of retrovirus-like particles in testicular teratocarcinomas. Journal of Urology 128:1055–1059
    [Google Scholar]
  10. Jaenisch R., Jähner D., Nobis P., Simm I., Löhler J., Harbers K., Grotkopp D. 1981; Chromosomal position and activation of retroviral genomes inserted into the germ line of mice. Cell 24:519–529
    [Google Scholar]
  11. Kalter S. S., Helmke R. J., Heberling R. L., Panigel M., Fowler A. K., Strickland J. E., Hellmann A. 1973; C-type particles in normal human placentas. Journal of the National Cancer Institute 50:1081–1084
    [Google Scholar]
  12. Kurth R., Mikschy U. 1978; Human antibodies reactive with purified envelope antigens of primate type-C tumor viruses. Proceedings of the National Academy of Sciences, U.S.A 75:5692–5696
    [Google Scholar]
  13. Kurth R., Teich N. M., Weiss R., Oliver R. T. D. 1977; Natural human antibodies reactive with primate type-C viral antigens. Proceedings of the National Academy of Sciences, U.S.A 74:1237–1241
    [Google Scholar]
  14. Kurth R., Löwer J., Löwer J., Harzmann R., Pfeiffer R., Schmidt C. G., Fogh J., Frank H. 1980; Oncovirus synthesis in human teratocarcinoma cultures and an increased anti-viral immune reactivity in corresponding patients. In Viruses in Naturally Occurring Cancers pp. 835–846 Essex M., Todaro G. J., Zurhausen H. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Leong J. C., Wood S. O., Lyford A. O., Levy J. A. 1984; Purification of a specific inhibitor of reverse transcriptase from human placenta. International Journal of Cancer 33:435–439
    [Google Scholar]
  16. Levy J. A., Hoffman A. D., Kramer S. M., Landis J. A., Shimabukuro J. M., Oshiro L. S. 1984; Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science 225:840–842
    [Google Scholar]
  17. Linney E., Davis B., Overhauser J., Chao E., Fan H. 1984; Non-function of a Moloney murine leukaemia virus regulatory sequence in F9 embryonal carcinoma cells. Nature; London: 308:470–472
    [Google Scholar]
  18. Löwer J., Davidson E. A., Teich N. M., Weiss R. A., Joseph A. P., Kurth R. 1981; Heterophil human antibodies recognize oncovirus envelope antigens: epidemiological parameters and immunological specificity of the reaction. Virology 109:409–417
    [Google Scholar]
  19. Löwer J. 1983 Nachweis Retrovirus-ähnlicher Partikel in humanen Teratocarzinomzellinien Ph.D. thesis University of Tübingen:
    [Google Scholar]
  20. Löwer J., Löwer J., Frank H., Harzmann R., Kurth R. 1984; Human teratocarcinomas cultured in vitro produce unique retrovirus-like viruses. Journal of General Virology 65:887–898
    [Google Scholar]
  21. Marsh M., Helenius A. 1980; Adsorptive endocytosis of Semliki Forest virus. Journal of Molecular Biology 142:439–454
    [Google Scholar]
  22. Periés J., Alves-Cordoso E., Canivet M., Debons-Guillemin M. C., Lasneret J. 1977; Lack of multiplication of ecotropic murine C-type viruses in mouse teratocarcinoma primitive cells: brief communication. Journal of the National Cancer Institute 59:463–465
    [Google Scholar]
  23. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. 1980; Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proceedings of the National Academy of Sciences, U.S.A 77:7415–7419
    [Google Scholar]
  24. Popovic M., Sarngadharan M. G., Read E., Gallo R. C. 1984; Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224:497–500
    [Google Scholar]
  25. Reddy E. S., Das M. R., Reddy E. P., Bhargava P. M. 1983; Inhibition of reverse transcriptase by seminal plasmin. Biochemical Journal 209:183–188
    [Google Scholar]
  26. Rho H. M., Poiesz B. J., Ruscetti F. W., Gallo R. C. 1981; Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell line. Virology 112:355–360
    [Google Scholar]
  27. Sarngadharan M. G., Robert-Guroff M., Gallo R. C. 1978; DNA polymerases of normal and neoplastic mammalian cells. Biochimica et biophysica acta 516:419–487
    [Google Scholar]
  28. Snyder H. w.Jr Fleissner E. 1980; Specificity of human antibodies to oncovirus glycoproteins: recognition of antigen by natural antibodies directed against carbohydrate structures. Proceedings of the National Academy of Sciences, U.S.A 77:1622–1626
    [Google Scholar]
  29. Teich N. M., Weiss R. A., Martin G. R., Lowy D. R. 1977; Virus infection of murine teratocarcinoma stem cell lines. Cell 12:973–982
    [Google Scholar]
  30. Wondrak E. M., Löwer J., Kurth R. 1986; Functional purification and enzymic characterization of the RNA-dependent DNA polymerase of human inmunodeficiency virus. Journal of General Virology 67:2791–2797
    [Google Scholar]
  31. Wong-Staal F., Gallo R. C. 1985; Human T-lymphotropic retroviruses. Nature; London: 317395–403
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-68-11-2807
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error