1887

Abstract

Summary

The nucleotide sequences of the adjacent genes coding for the F protein and 22K protein of the RSS-2 strain of human respiratory syncytial (RS) virus were determined from cDNA clones of genomic RNA. Comparison of these sequences and the inferred amino acid sequences of the F and 22K proteins with the corresponding published sequences of another subgroup A virus, the A2 strain of RS virus, reveals extensive overall homology (>95%) at both the nucleotide and amino acid levels, even though these two viruses were isolated 15 years apart in different continents. The intergenic region and the hydrophobic amino-terminal signal sequence of the F proteins of the two viruses, however, are much less conserved.

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1987-11-01
2022-01-17
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References

  1. Anderson L. J., Hierholzer J. C., Tsou C., Hendry R. M., Fernie B. F., Stone Y., Mcintosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  2. Cash P., Wunner W. H., Pringle C. R. 1977; A comparison of the polypeptides of human and bovine respiratory syncytial virus and murine pneumonia virus. Virology 82:369–379
    [Google Scholar]
  3. Chambers P., Millar N. S., Emmerson P. T. 1986; Nucleotide sequence of the gene encoding the fusion glycoprotein of Newcastle disease virus. Journal of General Virology 67:2685–2694
    [Google Scholar]
  4. Collins P. L., Wertz G. W. 1983; cDNA cloning and transcriptional mapping of nine polyadenylated RNAs encoded by the genome of human respiratory syncytial virus. Proceedings of the National Academy of Sciences, U.S.A 80:3208–3212
    [Google Scholar]
  5. Collins P. L., Wertz G. W. 1985a; The envelope-associated 22K protein of human respiratory syncytial virus: nucleotide sequence of the mRNA and a related polytranscript. Journal of Virology 54:65–71
    [Google Scholar]
  6. Collins P. L., Wertz G. W. 1985b; The 1A protein gene of human respiratory syncytial virus: nucleotide sequence of the mRNA and a related polycistronic transcript. Virology 141:283–291
    [Google Scholar]
  7. Collins P. L., Wertz G. W. 1985c; Nucleotide sequence of the IB and 1C nonstructural protein mRNAs of human respiratory syncytial virus. Virology 143:442–451
    [Google Scholar]
  8. Collins P. L., Huang Y. T., Wertz G. W. 1984a; Identification of a tenth mRNA of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes. Journal of Virology 49:572–578
    [Google Scholar]
  9. Collins P. L., Huang Y. T., Wertz G. W. 1984b; Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proceedings of the National Academy of Sciences, U.S.A 81:7683–7687
    [Google Scholar]
  10. Collins P. L., Anderson K., Langer S. J., Wertz G. W. 1985; Correct sequence of the major nucleocapsid protein mRNA of respiratory syncytial virus. Virology 146:69–77
    [Google Scholar]
  11. Collins P. L., Dickens L. E., Buckler-White A., Olmsted R. A., Spriggs M. K., Camargo E., Coelingh K. V. W. 1986; Nucleotide sequences for the gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. Proceedings of the National Academy of Sciences, U.S.A 83:4594–4598
    [Google Scholar]
  12. Dickens L. E., Collins P. L., Wertz G. W. 1984; Transcriptional mapping of human respiratory syncytial virus. Journal of Virology 52:364–369
    [Google Scholar]
  13. Elango N., Venkatesan S. 1983; Amino acid sequence of respiratory syncytial virus capsid protein. Nucleic Acids Research 11:5941–5951
    [Google Scholar]
  14. Elango N., Satake M., Coligan J. E., Norrby E., Camargo E., Venkatesan S. 1985a; Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of FI subunit. Nucleic Acids Research 13:1559–1574
    [Google Scholar]
  15. Elango N., Satake M., Venkatesan S. 1985b; mRNA sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22K structural protein. Journal of Virology 55:101–110
    [Google Scholar]
  16. Gimenez H. B., Hardman N., Keir H. M., Cash P. 1986; Antigenic variation between human respiratory syncytial virus isolates. Journal of General Virology 67:863–870
    [Google Scholar]
  17. Grunstein M., Hogness D. S. 1975; Colony hybridisation: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences, U.S.A 72:3961–3965
    [Google Scholar]
  18. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  19. Lambden P. R. 1985; Nucleotide sequence of the respiratory syncytial virus phosphoprotein gene. Journal of General Virology 66:1607–1612
    [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Mufson M. A., Örvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  22. Norrby E., Mufson M. A., Sheshberadaran H. 1986; Structural differences between subtype A and B strains of respiratory syncytial virus. Journal of General Virology 67:2721–2329
    [Google Scholar]
  23. Pringle C. R. 1987; Paramyxoviruses and disease. in Molecular Basis of Virus Disease, Society for General Microbiology Symposium 40 pp. 51–90 Russell W. C., Almond J. W. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  25. Satake M., Venkatesan S. 1984; Nucleotide sequence of the gene encoding respiratory syncytial virus matrix protein. Journal of Virology 50:92–99
    [Google Scholar]
  26. Satake M., Elango N., Venkatesan S. 1984; Sequence analysis of the respiratory syncytial virus phosphoprotein gene. Journal of Virology 52:991–994
    [Google Scholar]
  27. Satake M., Coligan J. E., Elango N., Norrby E., Venkatesan S. 1985; Respiratory syncytial virus envelope glycoprotein (G) has a novel structure. Nucleic Acids Research 13:7795–7812
    [Google Scholar]
  28. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  29. Spriggs M. K., Collins P. L. 1986; Sequence analysis of the P and C protein genes of human parainfluenza virus type 3: patterns of amino acid sequence homology among paramyxovirus proteins. Journal of General Virology 67:2705–2719
    [Google Scholar]
  30. Trudel M., Nadon F., Seguin C., Dionne G., Lacroix M. 1987; Identification of a synthetic peptide as part of a major neutralization epitope of respiratory syncytial virus. Journal of General Virology 68:2273–2280
    [Google Scholar]
  31. Venkatesan S., Elango N., Chanock R. M. 1983; Construction and characterisation of cDNA clones for four respiratory syncytial viral genes. Proceedings of the National Academy of Sciences, U.S.A 80:1280–1284
    [Google Scholar]
  32. Walsh E. E., Brandriss M. W., Schlesinger J. J. 1985; Purification and characterization of the respiratory syncytial virus fusion protein. Journal of General Virology 66:409–415
    [Google Scholar]
  33. Walsh E. E., Cote P. J., Fernie B. F., Schlesinger J. J., Brandriss M. W. 1986; Analysis of the respiratory syncytial virus fusion protein using monoclonal and polyclonal antibodies. Journal of General Virology 67:505–513
    [Google Scholar]
  34. Ward K. A., Lambden P. R., Ogilvie M. M., Watt P. J. 1983; Antibodies to respiratory syncytial virus polypeptides and their significance in human infection. Journal of General Virology 64:1867–1876
    [Google Scholar]
  35. Wertz G. W., Collins P. L., Huang Y. T., Gruber C., Levine S., Ball L. A. 1985; Nucleotide sequence of the G protein of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Acajemy of Sciences, U.S.A 82:4075–4079
    [Google Scholar]
  36. Yanisch-Perron C., Viera J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13 mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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