1887

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Volume 68, Issue 11

Strain Variation of Respiratory Syncytial Virus Free

Abstract

Western blotting and immunoperoxidase staining with monoclonal antibodies were employed to analyse epitopic and polypepide molecular weight variation among isolates of respiratory syncytial (RS) virus collected in Newcastle between 1965 and 1986. One group of isolates resembled the A2 and Long prototype subgroup A strains of RS virus in possessing a P protein of 34000. Isolates in this subgroup showed two patterns of reactivity with subgroup A-specific monoclonal antibodies to the G glycoprotein and 22K protein. Isolates with both reactivity patterns were isolated throughout the period studied. Isolates in the second group resembled the 8/60 subgroup B prototype strain in their lack of reactivity to subgroup A-specific monoclonal antibodies but were heterogeneous in P protein molecular weight. The earliest isolate only, made in 1965, possessed a P protein of 31000 resembling the prototype strain. All subsequent subgroup B isolates possessed a higher , 33000, P protein. Overall, subgroup A viruses were isolated most frequently although subgroup B strains may have predominated in some epidemics.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): MAbs , RS virus and subgroups
© Society for General Microbiology 1987
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1987-11-01
2024-04-25
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References

  1. Akerlind B., Norrby E. 1986; Occurrence of respiratory syncytial virus subtypes A and B strains in Sweden. Journal of Medical Virology 19:241–247
     [Google Scholar]
  2. Anderson L. J., Hierholzer J. C., Tsou C., Hendry R. M., Stone V., Mcintosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
     [Google Scholar]
  3. Beem M. 1967; Repeated infections with respiratory syncytial virus. Journal of Immunology 98:1115–1122
     [Google Scholar]
  4. Cash P., Wunner W. H., Pringle C. R. 1977; A comparison of the polypeptides of human and bovine respiratory syncytial viruses and murine pneumonia virus. Virology 82:369–379
     [Google Scholar]
  5. Chanock R., Roizman B., Moyers R. 1957; Recovery from infants with respiratory illnesses of a virus related to chimpanzee coryza agent. Isolation, properties and characterization. American Journal of Hygiene 66:281–290
     [Google Scholar]
  6. Coates H. V., Kendrick L., Chanock R. M. 1963; Antigenic differences between two strains of respiratory syncytial virus. Proceedings of the Society for Experimental Biology and Medicine 112:958–964
     [Google Scholar]
  7. Coates H. V., Alling D. W., Chanock R. M. 1966; An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. American Journal of Epidemiology 83:299–313
     [Google Scholar]
  8. Gimenez H. B., Hardman N., Keir H. M., Cash P. 1986; Antigenic variation between human respiratory syncytial virus isolates. Journal of General Virology 67:863–870
     [Google Scholar]
  9. Henderson F. W., Collier A. M., Clyde W. A., Denny F. W. 1979; Respiratory syncytial virus infections, reinfections and immunity.A prospective longitudinal study in young children. New England Journal of Medicine 300:530–534
     [Google Scholar]
  10. Hendry R. M., Talis A. L., Godfrey E., Anderson L. J., Fernie B. F., Mcintosh K. 1986a; Concurrent circulation of antigenically distinct strains of respiratory syncytial virus during community outbreaks. Journal of Infectious Diseases 153:291–297
     [Google Scholar]
  11. Hendry R. M., Burns J. C., Prince G. A., Rodriguez W., Kim H. W., Wright P. J., Graham B. S., Walsh E. E., Mcintosh K., Murphy B. R. 1986b; Infant sera recognize antigenically distinct strains of RS virus. Pediatric Research A917:
     [Google Scholar]
  12. Hierholzer J. C., Hirsch M. 1979; Croup and pneumonia in human infants associated with a new strain of respiratory syncytial virus. Journal of Infectious Diseases 140:826–828
     [Google Scholar]
  13. Mufson M. A., Örvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory virus. Journal of General Virology 66:2111–2124
     [Google Scholar]
  14. Norrby E., Mufson M. A., Sheshberadaran H. 1986; Structural differences between subtype A and B strains of respiratory syncytial virus. Journal of General Virology 67:2721–2729
     [Google Scholar]
  15. Prince G. A., Horswood R. L., Koenig D. W., Chanock R. M. 1985; Antigenic analysis of a putative new strain of respiratory syncytial virus. Journal of Infectious Diseases 151:634–637
     [Google Scholar]
  16. Pringle C. R., Cross A. 1978; Neutralization of respiratory syncytial virus by cat serum. Nature; London: 276501–502
     [Google Scholar]
  17. Routledge E. G., Mcquillin J., Samson A. C. R., Toms G. L. 1985; The development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence. Journal of Medical Virology 15:305–320
     [Google Scholar]
  18. Routledge E. G., Willcocks M. M., Samson A. C. R., Scott R., Toms G. L. 1986; Respiratory syncytial virus glycoprotein expression in human and simian cell lines. Journal of General Virology 67:2059–2064
     [Google Scholar]
  19. Routledge E. G., Willcocks M. M., Morgan L., Samson A. C. R., Scott R., Toms G. L. 1987; Heterogeneity of the respiratory syncytial virus 22K protein revealed by Western blotting with monoclonal antibodies. Journal of General Virology 68:1209–1215
     [Google Scholar]
  20. Samson A. C. R., Willcocks M. M., Routledge E. G., Morgan L. A., Toms G. L. 1986; A neutralizing monoclonal antibody to respiratory syncytial virus which binds to both F1 and F2 components of the fusion protein. Journal of General Virology 67:1479–1483
     [Google Scholar]
  21. Stott E. J., Taylor G. 1985; Respiratory syncytial virus.Brief review. Archives of Virology 84:1–52
     [Google Scholar]
  22. Taylor G., Stott E. J., Bew M., Fernie B. F., Cote P. J., Collins A. P., Hughes M., Jebbet J. 1984; Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52:137–142
     [Google Scholar]
  23. Wulff H., Kidd P., Wenner H. A. 1964; Respiratory syncytial virus: observations on antigenic heterogeneity. Proceedings of the Society for Experimental Biology and Medicine 115:240–243
     [Google Scholar]
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Strain Variation of Respiratory Syncytial Virus
J. Gen. Virol. 68, 2781 (1987); https://doi.org/10.1099/0022-1317-68-11-2781
/content/journal/jgv/10.1099/0022-1317-68-11-2781
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