1887

Abstract

Summary

Infection of a cowpea line by cowpea severe mosaic virus (CPSMV) was inhibited by cowpea mosaic virus (CPMV) even though the plants were immune to CPMV. The inhibition was dose-dependent and was complete if CPMV was added to the inoculum in a 50-fold excess over CPSMV. Isolated CPMV RNA inhibited infection by CPSMV or by isolated CPSMV RNA. CPMV particles devoid of RNA (top component), u.v.-inactivated but intact CPMV particles or u.v.-inactivated CPMV RNA did not inhibit infection by CPSMV. Bottom component particles of CPMV, but not middle component particles, inhibited CPSMV to the same extent as unfractionated CPMV. These findings strongly suggest that the interference is not due to competition between the particles or the RNAs of the two viruses for infectible sites. It may be that CPMV infects cells of the ‘immune’ host subliminally and that the inhibition is associated with replication of at least bottom component RNA of CPMV.

Keyword(s): CPMV , CPSMV and interference
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/content/journal/jgv/10.1099/0022-1317-68-11-2751
1987-11-01
2024-04-25
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References

  1. Agrawal H. O. 1964; Identification of cowpea mosaic virus isolates. MededelingenLandbouwhogeschoolWageningen 64(5):
    [Google Scholar]
  2. Beier H., Bruening G., Russell M. L., Tucker C. L. 1979; Replication of cowpea mosaic virus in protoplasts isolated from immune lines of cowpeas. Virology 95:165–175
    [Google Scholar]
  3. Bruening G., Lee S.-L., Beier H. 1979; Immunity to plant virus infection. In Plant Cell and Tissue Culture, Principles and Applications pp. 421–440 Sharp W. R., Larsen P. O., Paddock E. F., Raghavan V. Edited by Columbus: Ohio State University Press;
    [Google Scholar]
  4. Cooper J. I., Jones A. T. 1983; Responses of plants to viruses: proposals for the use of terms. Phytopathology 73:127–128
    [Google Scholar]
  5. Dijkstra J., Van Beek N. A. M., Lohuis D., Van Helden M., Meijer R. 1987; Is a helper factor necessary for infection of cowpea protoplasts with blackeye cowpea mosaic virus?. Netherlands Journal of Plant Pathology 93:43–47
    [Google Scholar]
  6. Eastwell K. C., Kiefer M. C., Bruening G. 1983; Immunity of cowpeas to cowpea mosaic virus. In Plant Molecular Biology, UCLA Symposium on Molecular Cell Biology 12 pp. 201–211 Goldberg R. B. Edited by New York: Alan R. Liss;
    [Google Scholar]
  7. Fuller F. J., Marcus P. I. 1980; Sindbis virus. I. Gene order of translation in vivo . Virology 107:441–451
    [Google Scholar]
  8. Goldbach R. 1988; Comoviruses: molecular biology and replication. In The Plant Viruses: Viruses with Bipartite RNA Genomes and Isometric Particles Harrison B. D., Murant A. F. Edited by New York: Plenum Press; in press
    [Google Scholar]
  9. Hibi T., Rezelman G, Van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
    [Google Scholar]
  10. Kassanis B. 1963; Interactions of viruses in plants. Advances in Virus Research 10:219–255
    [Google Scholar]
  11. Lindner R. C., Kirkpatrick H. C., Weeks T. E. 1959; Some factors affecting the susceptibility of cucumber cotyledons to infection by tobacco mosaic virus. Phytopathology 49:78–88
    [Google Scholar]
  12. Patel P. N. 1982; Genetics of cowpea reactions to two strains of cowpea mosaic virus from Tanzania. Phytopathology 72:460–466
    [Google Scholar]
  13. Polson A., Von Wechmar M. B. 1980; A novel way to transmit plant viruses. Journal of General Virology 51:179–181
    [Google Scholar]
  14. Rezelman G., Franssen H. J., Goldbach R. W., Ie T. S., Van Kammen A. 1982; Limits to the independence of bottom component RNA of cowpea mosaic virus. Journal of General Virology 60:335–342
    [Google Scholar]
  15. Rottier P. J. M., Rezelman G., Van Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  16. Snedecor G. W., Cochran W. G. 1982 Statistical Methods, 7th edn. Ames: Iowa State University Press;
    [Google Scholar]
  17. Sulzinski M. A., Zaitlin M. 1972; Tobacco mosaic virus replication in resistant and susceptible plants: in some resistant species virus is confined to a small number of initially infected cells. Virology 121:12–19
    [Google Scholar]
  18. Van Beek N. A. M., Derksen A. C. G., Dijkstra J. 1985; Polyethylene glycol-mediated infection of cowpea protoplasts with Sonchus yellow net virus. Journal of General Virology 66:551–557
    [Google Scholar]
  19. Van Kammen A. 1967; Purification and properties of the components of cowpea mosaic virus. Virology 31:633–642
    [Google Scholar]
  20. Williams R. J. 1975; Diseases of cowpea (Vigna unguiculata (L.)Walp.) in Nigeria. Pest Articles and News Summaries 21:253–267
    [Google Scholar]
  21. Wu G.-J., Bruening G. 1971; Two proteins from cowpea mosaic virus. Virology 46:596–612
    [Google Scholar]
  22. Wu J.-H., Rappaport I. 1961; An analysis of the interference between two strains of tobacco mosaic virus on Phaseolus vulgaris L. Virology 14:259–263
    [Google Scholar]
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