Sigma virus, the hereditary agent of a CO2-induced paralysis of Drosophila, is classified as a rhabdovirus on a molecular basis. We have purified its genome which after 32P-labelling was used as a probe to detect mRNAs in infected cells. A cDNA copy of the entire coding region of the glycoprotein mRN A was cloned. Nucleotide and deduced amino acid sequences were determined and compared to previously known sequences of other rhabdovirus glycoproteins to determine the relatedness of Sigma virus to other viruses of this group.
BerkaloffA., BreglianoJ. C., OhanessianA.1965; Mise en évidence de virions dans des drosophiles infectées par le virus héréditaire sigma. Compte Rendu hebdomadaire des séances de l’Académie des sciences 260:5956–5959
BishopD. H. L., AalestadH. G., ClarkH. F., FlamandA., ObijeskyJ. F., RepikP., RoyP.1975; Evidence for sequence homology and genome size of rhabdovirus RNAs. In Negative Strand Viruses1 pp 259–292BarryR. D., MahyB. W. I. Edited by New York: Academic Press;
BrunG., PlusN.1980; The viruses of Drosophila. In The Genetics and Biology of Drosophila2d pp 625–702AshburnerM., WrightT. R. F. Edited by New York: Academic Press;
BussereauF.1970; Etude du symptôme de la sensibilité au CO2 produit par le virus sigma chez la drosophile. II. Evolution comparée du rendement des centres nerveux et de divers organes après inoculation dans l’abdomen et dans le thorax. Annales de l’Institut Pasteur 118:626–645
BussereauF.1973; Etude du symptôme de la sensibilité au C02 produit par le virus de la stomatite vésiculaire chez Drosophila melanogaster. III. Souches de différents sérotypes. Annales de Microbiologie 124A:535–554
BussereauF.1975; The CO2 sensitivity induced by two rhabdoviruses, Piry and Chandipura, in Drosophila melanogaster. Annales de Microbiologie 126B:389–403
DayhoffM. O., HuntL. T., Hurst-CalderoneS.1978; Composition of proteins. In Atlas of Protein Sequence and Structure5 supplement 3 pp 363–373DayhoffM. O. Edited by Washington: National Biomedical Research Foundation;
GallioneC. J., RoseJ. K.1983; Nucleotide sequence of a cDNA clone encoding the entire glycoprotein from the New Jersey serotype of vesicular stomatitis virus. Journal of Virology 46:162–169
GallioneC. J., GreeneJ. R., IversonL. E., RoseJ. K.1981; Nucleotide sequences of the mRNAs encoding the vesicular stomatitis virus N and NS proteins. Journal of Virology 39:529–535
GillD. S., BanerjeeA. K.1986; Complete nucleotide sequence of the matrix protein mRNA of vesicular stomatitis virus (New Jersey serotype). Virology 150:308–312
GranthamR., GreenlandT., LouailS., MouchiroudD., PratoJ. L., GouyM., GautierG.1985; Molecular evolution of viruses as seen by nucleic acid sequence study. Bulletin de l’Institut Pasteur 83:95–148
HinkW. F.1980; The 1979 compilation of invertebrate cell lines and culture media. In Invertebrate Systems in vitro pp 553–578KurstakE., MaramoroschK., DubendorferA. Edited by Amsterdam: Elsevier/North- Holland;
KiuchiA., RoyP.1984; Comparison of the primary sequence of spring viremia of carp virus M protein with that of vesicular stomatitis virus. Virology 134:238–243
KuceraP., DolivoM., CoulonP., FlamandA.1985; Pathways of the early propagation of virulent and avirulent rabies strains from the eye to the brain. Journal of Virology 55:158–162
KushnerS. R.1978; Scientific development and practical application. In Genetic Engineering pp 17–23BoyerH. W., NicosiaS. Edited by Amsterdam: Elsevier/North-Holland;
LeppertM., RittenhouseL., PerraultJ., SummersD. F., KolakofskyD.1979; Plus and minus strand leader RNAs in negative strand virus-infected cells. Cell 18:735–747
L’HeritierPH, TeissierG.1937; Une anomalie physiologique h6r6ditaire chez la Drosophile. Compte rendu hebdomadaire des séances de l’Acadbmie des sciences 205:1099–1101
NeubergerA., GottschalkA., MarshallR. D., SpiroR. G.1972; Carbohydrate-peptide linkages in glycoproteins and methods for their elucidation. In Glycoproteins: Their Composition, Structure and Function pp 450–490GottschalkA. Edited by Amsterdam: Elsevier;
Pierre-DeneubourgA. M.1982; Characteristics of the CO2 sensitivity syndrome in Drosophila melanogaster: introduction of non-permissive alleles of two ref genes in a maternal line stabilized for sigma virus. Annales de Virologie 133E:281–294
PrintzP.1967; Mise en évidence d'un variant du virus de la stomatite vésiculaire (souche Indiana) conférant une sensibilité retardée au gaz carbonique chez Drosophila melanogaster. Compte rendu hebdomadaire des séances de l’Académie des sciences 264:169–172
Richard-MolardC., BlondelD., WyersF., DezelleS.1984; Sigma virus : growth in Drosophila melanogaster cell culture; purification; protein composition and localization. Journal of General Virology 65:91–99
RoseJ. K., GallioneC. J.1981; Nucleotide sequences of the mRNAs encoding the vesicular stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions. Journal of Virology 39:519–528
RoseJ. K., KnifeD.1975; Nucleotide sequence complexities, molecular weights, and poly(A) content of the vesicular stomatitis virus mRNA species. Journal of Virology 15:994–1003
RoseJ.K., LodishH. F., BrockM. L.1977; Giant heterogeneous polyadenylic acid on vesicular stomatitis virus mRNA synthesized in vitro in the presence of S-adenosylhomocysteine. Journal of Virology 21:683–693
RoseJ. K., AdamsG. A., GallioneC. J.1984; The presence of cysteine in the cytoplasmic domain of the vesicular stomatitis virus glycoprotein is required for palmitate addition. Proceedings of the National Academy of Sciences U.S.A.: 812050–2054
SabatiniD. D., KreibichG., MorimotoT., AdesnikM.1982; Mechanisms for the incorporation of proteins in membranes and organelles. Journal of Cellular Biology 92:1–22
SchererG., TelfordJ., BaldariC., PirottaV.1981; Isolation of cloned genes differentially expressed at early and late stages of drosophila embryonic development. Developmental Biology 86:438–447
SchlegelR., WillinghamM. C., PastanI. N.1982; Saturable binding sites for vesicular stomatitis virus on the surface of Vero cells. Journal of Virology 43:871–875
SchubertM., HarmisonG. G., MeierE.1984; Primary structure of the vesicular stomatitis virus polymerase (L) gene: evidence for a high frequency of mutations. Journal of Virology 51:505–514
SeifI., CoulonP., RollinP. E., FlamandA.1985; Rabies virulence: effect on pathogenicity and sequence characterization of rabies virus mutations affecting antigenic site III of the glycoprotein. Journal of Virology 53:926–934
TordoN., PochO., ErmineA., KeithG., RougeonF.1986; Walking along the rabies genome: is the large GL intergenic region a remnant gene?. Proceedings of the National Academy of Sciences U.S.A.: 833914–3918
VieiraJ., MessingI.1982; The pUC plasmids and Ml3 mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268