The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa califomica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. Spodoptera frugiperda cells infected with the derived recombinant baculovirus produced and secreted 22 nm particles containing the hepatitis B surface antigen. The particles had morphological and antigenic properties identical to those of 22 nm particles isolated from the plasma of chronic active hepatitis patients.
KurodaK., HauserC., RottR., KlenkH.-D., DoerflerW.1986; Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector. EMBO Journal 5:1359–1365
MaedaS., KawaiT., ObinataM., FujiwaraH., HoriuchiT., SaekiY., SatoY., FurusawaM.1985; Production of human alpha-interferon in silkworms using a baculovirus vector. Nature; London: 315592–594
MatsuuraY., PosseeR. D., BishopD. H. L.1986; Expression of the S-coded genes of lymphocytic choriomeningitis arenavirus using a baculovirus vector. Journal of General Virology 67:1515–1529
MatsuuraY., PosseeR. D., OvertonH. A., BishopD. H. L.1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
MilichD. R., ThortonG. B., NeurathA. R., KentS. B., MichelM.-L., TiollaisP., ChisariF. V.1985; Enhanced immunogenicity of the Pre-S region of hepatitis B surface antigen. Science 228:1195–1198
MiyamotoC., SmithG. E., Farrell-TowtJ., ChizzoniteR., SummersM. D., JuG.1985; Production of human c-myc protein in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 5:2860–2865
OnoY., OndaH., SasadaR., IgarashiK., SuginoY., NishiokaK.1983; The complete nucleotide sequences of the cloned hepatitis B virus DNA; subtype adr and adw. Nucleic Acids Research 11:1747–1757
OvertonH. A., IharaT., BishopD. H. L.1987; Identification of the N and NSs proteins coded by the ambisense S RNA of Punta Toro phlebovirus using monospecific antisera raised to baculovirus expressed N and NSs proteins. Virology in press
PennockG. D., ShoemakerC., MillerL. K.1984; Strong and regulated expression OF Escherichia coli fS- galactosidase in insect cells with a baculovirus vector. Molecular and Cellular Biology 4:399–406
SmithG. E., SummersM. D., FraserM. J.1983; Production of human beta interferon in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 3:2156–2165
SmithG. E., JuG., EricsonB. L., MoscheraJ., LahnH.-W., ChizzoniteR., SummersM. D.1985; Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proceedings of the National Academy of Sciences U.S.A.: 828404–8408
ValenzuelaP., QuirogaM., ZaldivarJ., GrayP., RutterW. J.1985; The nucleotide sequence of the hepatitis B virus genome and the identification of the major viral genes. In Animal Virus Genetics pp 57–70FieldsB. N., JaenischR., FoxC. F. Edited by New York: Academic Press;