1887

Abstract

SUMMARY

Upon infection of cowpea mesophyll protoplasts with cowpea mosaic virus (CPM V), the only M RNA-encoded proteins detected so far have been the two capsid proteins VP37 and VP23. We now report the detection of a 60000 (60K) precursor to both capsid proteins in infected protoplasts cultured in the presence of zinc ions. Furthermore a M RNA-encoded 48K protein was detected in the membrane fraction of infected cells using antisera raised against synthetic peptides. The results obtained indicate that, just like the bottom component RNA, the M RNA of CPMV is translated into a polyprotein from which proteins are derived by proteolytic cleavage.

Keyword(s): capsid protein precursor , CPMV and M RNA
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-10-2577
1987-10-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/10/JV0680102577.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-10-2577&mimeType=html&fmt=ahah

References

  1. Bachmair A., Finley D., Varshavsky A. 1986; In vivo half-life of a protein is a function of its amino-terminal residue. Science 234:179–186
    [Google Scholar]
  2. Barany G., Merrifield R. B. 1980; Solid-phase peptide synthesis. In The Peptides 2 pp 1–24 Gross E., Merenhofer J. Edited by New York: Academic Press;
    [Google Scholar]
  3. Baron M. H., Baltimore D. 1982; Antibodies against the chemically synthesized genome-linked protein of poliovirus react with native virus-specific proteins. Cell 28:395–104
    [Google Scholar]
  4. Franssen H., Goldbach R., Broekhuysen M., Moerman M., Van Kammen A. 1982; Expression of middle- component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells. Journal of Virology 41:8–17
    [Google Scholar]
  5. Fransen H., Moerman M., Rezelman G., Goldbach R. 1984; Evidence that the 32,000-dalton protein encoded by bottom-component RNA of cowpea mosaic virus is a proteolytic processing enzyme. Journal of Virology 50:183–190
    [Google Scholar]
  6. Godefroy-Colburn T., Gagey M.-J., Berna A., Stussi-Garaud C. 1986; A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. Journal of General Virology 67:2233–2239
    [Google Scholar]
  7. Goldbach R., Krijt J. 1982; Cowpea mosaic virus-encoded protease does not recognize primary translation products of M RNAs from other comoviruses. Journal of Virology 43:1151–1154
    [Google Scholar]
  8. Goldbach R., Van Kammen A. 1985; Structure, replication and expression of the bipartite genome of cowpea mosaic virus. In Molecular Plant Virology 2 pp 83–120 Davies J. W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  9. Goldbach R., Rezelman G., Van Kammen A. 1980; Independent replication and expression of B-component RNA of cowpea mosaic virus. Nature; London: 286297–300
    [Google Scholar]
  10. Klootwijk J., Klein L, Van Kammen A. 1977; Cowpea mosaic virus RNAs have neither m7 GpppN … nor mono-, di-, or triphosphates at their 5ʹ-ends. Cell 11:73–82
    [Google Scholar]
  11. Kozak M. 1984; Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Research 12:857–872
    [Google Scholar]
  12. Leonard D. A., Zaitlin M. 1982; A temperature-sensitive strain of tobacco mosaic virus defective in cell-to-cell movement generates an altered viral-coded protein. Virology 117:416–424
    [Google Scholar]
  13. Lomonossoff G. P., Shanks M. 1983; The nucleotide sequence of cowpea mosaic virus B RNA. EMBO Journal 2:2253–2258
    [Google Scholar]
  14. Meyer M., Hemmer O., Mayo M. A., Fritsch C. 1986; The nucleotide sequence of tomato black ring virus RNA- 2. Journal of General Virology 67:1257–1271
    [Google Scholar]
  15. Muller S., Plaue S., Couppez M., Van Regenmortal M. H. V. 1986; Comparison of different methods for localizing antigenic regions in histone H2A. Molecular Immunology 23:593–601
    [Google Scholar]
  16. Ohno T., Takamatsu T., Meshi T., Okada Y., Nishiguchi M., Kiho Y. 1983; Single amino acid substitution in 30K protein of TMV defective in virus transport function. Virology 131:255–258
    [Google Scholar]
  17. Pelham H. R. B. 1979; Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates. Virology 96:463–477
    [Google Scholar]
  18. Peng X. X., Shih D. S. 1984; Proteolytic processing of the proteins translated from the bottom-component RNA of cowpea mosaic virus. Journal of Biological Chemistry 259:3197–3201
    [Google Scholar]
  19. Rezelman G., Goldbach R., Van Kammen A. 1980; Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts. Journal of Virology 36:366–373
    [Google Scholar]
  20. Rezelman G., Franssen H. J., Goldbach R. W., Ie T. S., Van Kammen A. 1982; Limits to the independence of bottom component RNA of cowpea mosaic virus. Journal of Genera! Virology 60:335–342
    [Google Scholar]
  21. Shanks M., Stanley J., Lomonossoff G. P. 1986; The primary structure of red clover mottle virus middle component RNA. Virology 155:697–706
    [Google Scholar]
  22. Van Kammen A. 1967; Purification and properties of the components of cowpea mosaic virus. Virology 31:635–642
    [Google Scholar]
  23. Van Wezenbeek P., Verver J., Harmsen J., Vos P., Van Kammen A. 1983; Primary structure and gene organisation of the middle-component RNA of cowpea mosaic virus. EMBO Journal 2:941–946
    [Google Scholar]
  24. Vos P., Verver J., Van Wezenbeek P., Van Kammen A., Goldbach R. 1984; Study of the genetic organisation of a plant viral RNA genome by in vitro expression of a full-length DNA copy. EMBO Journal 3:3049–3053
    [Google Scholar]
  25. Wellink J., Rezelman G., Goldbach R., Beyreuther K. 1986; Determination of the proteolytic processing sites in the polyprotein encoded by the B-RNA of cowpea mosaic virus. Journal of Virology 59:50–58
    [Google Scholar]
  26. Wellink J., Jaegle M., Goldbach R. 1987; Detection of a novel protein encoded by the B-RNA of cowpea mosaic virus, using antibodies raised against a synthetic peptide. Journal of Virology 61:236–238
    [Google Scholar]
  27. Zabel P., Moerman M., Van Straaten F., Goldbach R., Van Kammen A. 1982; Antibodies against the genome-linked protein VPg of cowpea mosaic virus recognize a 60,000-dalton precursor polypeptide. Journal of Virology 41:1083–1088
    [Google Scholar]
  28. Zimmern D., Hunter T. 1983; Point mutation in the 30K open reading frame of TMV implicated in temperature-sensitive assembly and local lesion spreading of mutant Ni 2519. EMBO Journal 2:1893–1900
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-10-2577
Loading
/content/journal/jgv/10.1099/0022-1317-68-10-2577
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error