1887

Abstract

SUMMARY

Primary brain cell cultures prepared from newborn mice were infected with Semliki Forest virus (SFV). The effects of interferon (IFN- ) treatment on SFV replication, SFV and major histocompatibility complex (MHC) class I antigen expression, and susceptibility to lysis by SFV-specific cytotoxic T lymphocytes (CTL) were determined.

The IFN- treatment prevented replication of SFV as determined by incorporation of [H]uridine into SFV RN A and very markedly reduced the expression of SFV antigens on the cell surface as determined by lysis with antibody and complement or indirect immunofluorescence. However, IFN-aJ? increased expression of MHC class I antigens, measured by indirect immunofluorescence and as assessed indirectly by susceptibility to killing by alloreactive T cell lines. SFV infection had no effect on MHC class I expression in either IFN- -treated or -untreated cells. The infected IFN--untreated brain cells were susceptible to killing by the CTL at effector/target ratios in the range 3 to 30. The killing was MHC antigen-restricted, and uninfected cells were not killed. A target cell (YAC) highly susceptible to natural killer cell cytotoxicity was not killed by the CTL. IFN- treatment prior to SFV infection resulted in an augmentation of lysis by the CTL, indicating that even where SFV antigen expression is reduced, in the context of enhanced MHC class I expression brain cells remain susceptible to CTL killing.

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1987-01-01
2024-03-29
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References

  1. Atkins G. J., Johnston M. D., Westmacott L. M., Burke D. C. 1974; Induction of interferon in chick cells by temperature-sensitive mutants of Sindbis virus. Journal of General Virology 25:381–390
    [Google Scholar]
  2. Atkins G. J., Sheahan B. J., Dimmock N. J. 1985; Semliki Forest virus infection of mice: a model for genetic and molecular analysis of viral pathogenicity. Journal of General Virology 66:395–408
    [Google Scholar]
  3. Berger M. 1980; Humoral and cell-mediated immune mechanisms in the production of pathology in avirulent Semliki Forest virus encephalitis. Infection and Immunity 30:244–253
    [Google Scholar]
  4. Blackman M. J., Morris A. G. 1984; Gamma interferon production and cytotoxicity of spleen cells from mice infected with Semliki Forest virus. Journal of General Virology 65:955–961
    [Google Scholar]
  5. Blackman M., Morris A. 1985; The effect of interferon treatment of targets on susceptibility to cytotoxic T-lymphocyte killing: augmentation of allogeneic killing and virus-specific killing relative to viral antigen expression. Immunology 56:451–457
    [Google Scholar]
  6. Bradish C. J., Allner K., Maber H. B. 1971; The virulence of original and derived strains of Semliki Forest virus for mice, guinea-pigs and rabbits. Journal of General Virology 12:141–160
    [Google Scholar]
  7. Bruce C., Chapman J., Suckling A., Rumsby M. 1984; Infection of rat brain primary cell cultures with an avirulent A7 strain of Semliki Forest virus. Journal of the Neurological Sciences 66:77–90
    [Google Scholar]
  8. Bukowski J. F., Walsh R. M. 1985; Interferon enhances the susceptibility of virus infected fibroblasts to cytotoxic T-cells. Journal of Experimental Medicine 161:257–262
    [Google Scholar]
  9. Fazakerley J. K., Amor S., Webb H. E. 1983; Reconstitution of Semliki Forest virus infected mice induces immune mediated pathological changes in the CNS. Clinical and Experimental Immunology 52:115–120
    [Google Scholar]
  10. Fierz W., Endler B., Reske K., Wekerle H., Fontana A. 1985; Astrocytes as antigen presenting cells.I.Induction of la antigen expression on astrocytes by T-cells via immune interferon and its effect on antigen presentation. Journal of Immunology 134:3785–3793
    [Google Scholar]
  11. Flyer D., Burakoff S., Faller D. 1985; Retrovirus-induced changes in major histocompatability complex antigen expression influences susceptibility to lysis by cytotoxic T-lymphocytes. Journal of Immunology 135:2287–2292
    [Google Scholar]
  12. Gates M. C., Sheahan B. J., O’Sullivan M. A., Atkins G. J. 1985; The pathogenicity of the A7, M9 and L10 strains of Semliki Forest virus for weanling mice and primary mouse brain cell cultures. Journal of General Virology 66:2365–2373
    [Google Scholar]
  13. Jacobs L., O’Mally J., Freeman A., Ekes R. 1982; Intrathecal interferon reduces exacerbations of multiple sclerosis. Science 214:1026–1028
    [Google Scholar]
  14. Massa P. T., Dorries R., Ter Meulen V. 1986; Viral particles induce la antigen expression on astrocytes. Nature; London: 320543–546
    [Google Scholar]
  15. Morris A. G., Lin Y-L., Askonas B. A. 1982; Immune interferon release when a cloned cytotoxic T-cell line meets its correct influenza-infected target cell. Nature; London: 295150–152
    [Google Scholar]
  16. Reznikoff C. A., Brankow D. W., Heidelberger C. 1973; Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to past-confluence inhibition of division. Cancer Research 33:3231–3238
    [Google Scholar]
  17. Vitetta E. S., Capra J. D. 1978; The protein products of the murine 17th chromosome: genetics and structure. Advances in Immunology 26:147–193
    [Google Scholar]
  18. Wong G., Bartlett P., Clark-Lewis I., Battye F., Schrader J. 1983; Inducible expression of H-2 and la antigens on brain cells. Nature; London: 310688–691
    [Google Scholar]
  19. Zamvil S., Nelson P., Trotter J., Mitchell D., Knobler R., Fritz R., Steinman L. 1985; T-cell clones specific for myelin basic protein induce chronic relapsing paralysis and demyelination. Nature; London: 317:355–359
    [Google Scholar]
  20. Zinkernagel R. M., Doherty P. C. 1974; Restriction of in vitro T-cell mediated cytotoxicity in lymphocytic choriomeningitis within a syngeneic or semiallogeneic system. Nature; London: 248701–702
    [Google Scholar]
  21. Zinkernagel R. M., Doherty P. C. 1979; MHC-restricted cytotoxic T-cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction specificity, function and responsiveness. Advances in Immunology 27:52–180
    [Google Scholar]
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