Stability of a Bacterial Gene in a Bovine Papillomavirus-based Shuttle Vector Maintained Extrachromosomally in Mammalian Cells Free

Abstract

SUMMARY

In order to analyse the stability of cloned genes in a viral vector we have constructed a shuttle vector based on bovine papillomavirus and the gene . Propagation of this vector in mouse C127 cells and analysis of vector sequences in bacteria produced no detectable mutations in the gene in over 6137 clones analysed. This is 100-fold less than the mutation frequency observed when the same and similar target genes are replicated in monkey COS cells using a simian virus 40- based shuttle vector.

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1987-01-01
2024-03-29
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