1887

Abstract

SUMMARY

Regions of homology on the physical maps of multiple-nucleocapsid nuclear polyhedrosis virus (SeMNPV-25), an MNPV genomic variant, and (SfMNPV-2) baculovirus DNAs were identified by reciprocal DNA-DNA blot hybridization under conditions of an effective temperature of − 25 °C. In addition, cloned fragments of the viral genome which contained the homologous regions were used in hybridization experiments to confirm, refine and correlate the regions of the two physical maps. Five homologous regions conserved between the two physical maps were identified. When the stringency of the hybridization was increased ( − 20 °C), only two of the original regions were identified by blot hybridization. One of the two regions contained the polyhedrin gene, and the other region was not associated with any known viral function. The five regions did not overlap with the intragenic homologous sequence (hrl to hr5) regions on the SeMNPV-25 map or with restriction endonuclease variant (vl to vIV) regions on the SeMNPV-25 and SfMNPV-2 maps. The degree of similarity in the genomic organization of these two baculoviruses is discussed.

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1987-01-01
2021-10-17
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References

  1. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  2. Bolivar F., Bachman K. 1979; Plasmids of Escherichia coli as cloning vectors. Methods in Enzymology 68:245–267
    [Google Scholar]
  3. Brown S. E., Maruniak J. E., Knudson D. L. 1984; Physical map of SeMNPV baculovirus DNA: an AcMNPV genomic variant. Virology 136:235–240
    [Google Scholar]
  4. Brown S. E., Maruniak J. E., Knudson D. L. 1985; Baculovirus (MNPV) genomic variants: characterization of Spodoptera exempta MNPV DNAs and comparison with other Autographa califomica MNPV DNAs. Journal of General Virology 66:2431–2441
    [Google Scholar]
  5. Cochran M. A., Faulkner P. 1983; Location of homologous DNA sequences interspersed at five regions in the baculovirus AcMNPV genome. Journal of Virology 45:961–970
    [Google Scholar]
  6. Cohen S. N., Chang A. C., Hsu L. 1972; Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proceedings of the National Academy of Sciences, U.S.A 69:2110–2114
    [Google Scholar]
  7. Godson G. N., Vapnek D. 1973; A simple method of preparing large amounts of 𝜙X174 RFI supercoiled DNA. Biochimica et biophysica acta 299:516–520
    [Google Scholar]
  8. Howley P. M., Israel M. A., Law M., Martin M. A. 1979; A rapid method for detecting and mapping homology between heterologous DNAs. Journal of Biological Chemistry 254:4876–4883
    [Google Scholar]
  9. Jewell J. E., Miller L. K. 1980; DNA sequence homology relationships among six lepidopteran nuclear polyhedrosis viruses. Journal of General Virology 48:161–175
    [Google Scholar]
  10. Kelly D. c. 1977; The DNA contained by nuclear polyhedrosis viruses isolated from four Spodoptera Sp. (Lepidoptera, Noctuidae); genome size and homology assessed by DNA reassociation kinetics. Virology 76:468–471
    [Google Scholar]
  11. Knudson D. L. 1980; Specificity of baculoviruses. In Safety Aspects of Baculoviruses as Biological Insecticides: Symposium 13-15 November 1978 pp. 241–256 Miltenburger H. G. Edited by Bonn: Bundesministerium für Forschung und Technologie;
    [Google Scholar]
  12. Knudson D. L., Buckley s. M. 1977; Invertebrate cell culture methods for the study of invertebrate-associated animal viruses. In Methods in Virology 6 pp. 323–391 Maramorosch K., Koprowski H. Edited by New York: Academic Press;
    [Google Scholar]
  13. Knudson D. L., Tinsley T. W. 1974; Replication of a nuclear polyhedrosis in a continuous cell culture of Spodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus. Journal of Virology 14:934–944
    [Google Scholar]
  14. Knudson D. L., Tinsley T. W. 1978; Replication of a nuclear polyhedrosis virus in a continuous cell line of Spodoptera frugiperda: partial characterization of the viral DNA, comparative DNA-DNA hybridization, and patterns of DNA synthesis. Virology 87:42–57
    [Google Scholar]
  15. Leisy D. J., Rohrmann G. F., Beaudreau G. s. 1984; Conservation of genome organization in two multicapsid nuclear polyhedrosis viruses. Journal of Virology 52:699–702
    [Google Scholar]
  16. Lubbert H., Doerfler W. 1984; Mapping of early and late transcripts encoded by the Autographa califomica nuclear polyhedrosis virus genome: is viral RNA spliced. Journal of Virology 50:497–506
    [Google Scholar]
  17. Maruniak J. E., Summers M. D. 1978; Comparative peptide mapping of baculovirus polyhedrins. Journal of Invertebrate Pathology 32:196–201
    [Google Scholar]
  18. Maruniak J. E., Brown S. E., Knudson D. L. 1984; Physical maps of SfMNPV baculovirus DNA and its genomic variants. Virology 136:221–234
    [Google Scholar]
  19. Rohrmann G. F., Mcparland R. H., Martignoni M. E., Beaudreau G. S. 1978; Genetic relatedness of two nucleopolyhedrosis viruses pathogenic for Orgyia pseudotsugata. Virology 84:213–217
    [Google Scholar]
  20. Rohrmann G. F., Martignoni M. E., Beaudreau G. S. 1982; DNA sequence homology between Autographa califomica and Orgyia pseudotsugata nuclear polyhedrosis viruses. Journal of General Virology 62:137–143
    [Google Scholar]
  21. Smith G. E., Summers M. D. 1981; Application of a novel radioimmunoassay to identify baculovirus structural proteins that share interspecies antigenic determinants. Journal of Virology 39:125–137
    [Google Scholar]
  22. Smith G. E., Summers M. D. 1982; DNA homology among subgroup A, B, and C baculoviruses. Virology 123:393–406
    [Google Scholar]
  23. Smith G. E., Vlak J. M., Summers M. D. 1982; In vitro translation of Autographa califomica nuclear polyhedrosis virus early and late mRNAs. Journal of Virology 44:199–208
    [Google Scholar]
  24. Summers M. D., Smith G. E., Knell J. D., Burand J. P. 1980; Physical maps of Autographa califomica and Rachiplusia ou nuclear polyhedrosis virus recombinants. Journal of Virology 34:693–703
    [Google Scholar]
  25. Vlak J. M., Smith G. E., Summers M. D. 1981; Hybridization selection and in vitro translation of Autographa califomica nuclear polyhedrosis virus mRNA. Journal of Virology 40:762–771
    [Google Scholar]
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