1887

Abstract

SUMMARY

Two strains of red clover necrotic mosaic virus (TpM 34 and TpM 48) were distinguished by their serological relationship, by the symptoms they induced in selected host plants and by Northern hybridization analysis. Purified RNA 1 and RNA 2 of the two strains were inoculated to the leaves of and in all possible combinations. It was demonstrated that a heterologous mixture containing RNA 1 of TpM 34 and RNA 2 of TpM 48 was infectious, resulting in lesion development whereas the reciprocal combination was not. The infectious pseudorecombinant was isolated by several single lesion transfers in and its genetic nature was confirmed by serology and Northern hybridization analysis. Inoculation of the pseudorecombinant and the two parental strains to five selected host plants revealed that symptom expression was determined by RNA 1.

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1987-01-01
2024-04-20
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References

  1. Garger S. J., Turpen T., Carrington J. C., Morris T. J., Jordan R. L., Dodds J. A., Grill L. K. 1983; Rapid detection of plant RNA viruses by dot blot hybridization. Plant Molecular Biology Reporter 1:21–25
    [Google Scholar]
  2. Ghabrial S. A., Lister R. M. 1973; Coat protein and symptom specification in tobacco rattle virus. Virology 52:1–12
    [Google Scholar]
  3. Gould A. R., Francki R. I. B., Hatta T., Hollings M. 1981; The bipartite genome of red clover necrotic mosaic virus. Virology 108:499–506
    [Google Scholar]
  4. Haseltine W. A., Baltimore D. 1976; Size of murine RNA tumor virus specific nuclear RNA molecules. Journal of Virology 19:331–337
    [Google Scholar]
  5. Hollings M., Stone O. M. 1977; Red clover necrotic mosaic virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses181
    [Google Scholar]
  6. Huber R., Rezelman G., Hibi T., Van Kammen A. 1977; Cowpea mosaic virus infection of protoplasts from Samsun tobacco leaves. Journal of General Virology 34:315–323
    [Google Scholar]
  7. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  9. Morris-Krsinich B. A. M., Forster R. L. S., Mossop D. W. 1983; Translation of red clover necrotic mosaic virus RNA in rabbit reticulocyte lysate: identification of the virus coat protein cistron on the larger RNA strand of the bipartite genome. Virology 124:349–356
    [Google Scholar]
  10. Musil M., Gallo J. 1982; Serotypes of red clover necrotic mosaic virus. I. Characterization of three serotypes. Acta virologica 26:497–501
    [Google Scholar]
  11. Okuno T., Hiruki C., Rao D. V., Figueiredo G. C. 1983; Genetic determinants distributed in two genomic RNAs of sweet clover necrotic mosaic, red clover necrotic mosaic and clover primary leaf necrosis viruses. Journal of General Virology 64:1907–1914
    [Google Scholar]
  12. Osman T. A. M., Dodd S. M., Buck K. W. 1986; RNA 2 of red clover necrotic mosaic virus determines lesion morphology and systemic invasion in cowpea. Journal of General Virology 67:203–207
    [Google Scholar]
  13. Randles J. W., Harrison B. D., Murant A. F., Mayo M. A. 1977; Packaging and biological activity of the two essential RNA species of tomato black ring virus. Journal of General Virology 36:187–193
    [Google Scholar]
  14. Rao A. L. N., Hatta T., Francki R. I. B. 1982; Comparative studies on tomato aspermy and cucumber mosaic viruses. VII. Serological relationships reinvestigated. Virology 116:318–326
    [Google Scholar]
  15. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:325–330
    [Google Scholar]
  16. Van Vloten-Doting L., Bos J. F., Jaspars E. M. J. 1977; Plant viruses with multipartite genomes. In Beltsville Symposium in Agriculture. I Virology in Agriculture pp. 47–61 Roberger J. A., Anderson J. D., Powell R. L. Edited by Totowa, N. J: Allanheld, Osmun & Co;
    [Google Scholar]
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