1887

Abstract

SUMMARY

The DNA of soybean chlorotic mottle virus (SoyCMV) has been isolated. When analysed by gel electrophoresis, the native DNA formed multiple bands resembling DNA from other caulimoviruses. The viral DNA was cloned in bacterial vectors. Cloned DNA was used to map restriction sites on the SoyCMV DNA. Comparison of cloned DNAs of SoyCMV and cauliflower mosaic virus (CaMV) by Southern transfer hybridization revealed the absence of significant sequence homology. Electrophoresis in denaturing gels showed that SoyCMV DNA had three single-strand discontinuities (‘gaps’), one in one strand and two in the other, and the positions of these gaps were mapped on the viral DNA. Potential primer sites for reverse transcription were detected in the cloned DNA by sequence analysis of regions around the gap sites. The results obtained definitely establish SoyCMV as a member of the caulimovirus group and suggest a replication mechanism for this virus similar to that described for CaMV.

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1987-01-01
2024-04-23
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References

  1. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  2. Canaday J., Guillemaut P., Weil J.-H. 1980; The nucleotide sequences of the initiator transfer RNAs from bean cytoplasm and chloroplasts. Nucleic Acids Research 8:999–1008
    [Google Scholar]
  3. Covey S. N. 1985; Organization and expression of the cauliflower mosaic virus genome. In Molecular Plant Virology II pp. 121–159 Davies J. W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  4. Donson J., Hull R. 1983; Physical mapping and molecular cloning of caulimovirus DNA. Journal of General Virology 64:2281–2288
    [Google Scholar]
  5. Drouin J. 1980; Cloning of human mitochondrial DNA in Escherichia coii. Journal of Molecular Biology 140:15–34
    [Google Scholar]
  6. Gardner R. C., Shepherd R. J. 1980; A procedure for rapid isolation and analysis of cauliflower mosaic virus DNA. Virology 106:159–161
    [Google Scholar]
  7. Gibson T. J. 1984 Studies on the Epstein-Barr virus genome Ph.D thesis University of Cambridge:
    [Google Scholar]
  8. Grimsley N., Hohn B., Hohn T., Walden R. 1986; Agroinfection, a novel route for plant viral infection using Ti plasmid. Proceedings of the National Academy of Sciences, U.S.A 83: in press
    [Google Scholar]
  9. Guilley H., Richards K. E., Jonard G. 1983; Observations concerning the discontinuous DNAs of cauliflower mosaic virus. EMBO Journal 2:277–282
    [Google Scholar]
  10. Howell S. H. 1985; The molecular biology of plant DNA viruses. CRC Critical Reviews in Plant Sciences 2(4):287–316
    [Google Scholar]
  11. Howell S. H., Walker L. L., Dudley R. K. 1980; Cloned cauliflower mosaic virus DNA infects turnips (Brassica rapa). Science 208:1265–1267
    [Google Scholar]
  12. Hull R. 1984; Caulimovirus group. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses295
    [Google Scholar]
  13. Hull R., Covey S. N. 1983; Does cauliflower mosaic virus replicate by reverse transcription?. Trends in Biochemical Sciences 8:119–121
    [Google Scholar]
  14. Hull R., Covey S. N., Stanley J., Davies J. W. 1979; The polarity of the cauliflower mosaic virus genome. Nucleic Acids Research 7:669–677
    [Google Scholar]
  15. Iwaki M., Isogawa Y., Tsuzuki H., Honda Y. 1984; Soybean chlorotic mottle, a new caulimovirus on soybean. Plant Disease 68:1009–1011
    [Google Scholar]
  16. McDonnell M. W., Simon M. N., Studier F. W. 1977; Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology 110:119–146
    [Google Scholar]
  17. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Maule A. J. 1985; Replication of caulimoviruses in plants and protoplasts. In Molecular Plant Virology II pp. 161–190 Davies J. W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  19. Maule A. J., Thomas C. M. 1985; Evidence from cauliflower mosaic virion DNA for additional discontinuities in the plus strand. Nucleic Acids Research 13:7359–7373
    [Google Scholar]
  20. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 100:255–266
    [Google Scholar]
  21. Pfeiffer P., Hohn T. 1983; Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects. Cell 33:781–789
    [Google Scholar]
  22. Richins R. D., Shepherd R. J. 1983; Physical maps of the genomes of dahlia mosaic virus and mirabilis mosaic virus - two members of the caulimovirus group. Virology 124:208–214
    [Google Scholar]
  23. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  24. Tantz D., Renz M. 1983; An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Analytical Biochemistry 132:14–19
    [Google Scholar]
  25. Turner D. S., Covey S. N. 1984; A putative primer for the replication of cauliflower mosaic virus by reverse transcription is virion-associated. FEBS Letters 165:285–289
    [Google Scholar]
  26. Vieira J., Messing J. 1982; The pUC plasmids, a M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  27. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:105–119
    [Google Scholar]
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