Expression of Reovirus Type 3 (Dearing) σ1 and σs Polypeptides in Free

Abstract

SUMMARY

The reovirus S1 gene codes for two polypeptides: l and s. In order to characterize the structure and function of the l polypeptide, we have expressed the 1 protein in The SI gene from mammalian reovirus type 3 (Dearing strain) and the variant K strain were subcloned into an expresssion vector containing the () promoter designed to express foreign gene products in efficiently. The hybrid plasmids, upon induction with isopropyl---thiogalactopyranoside, expressed two polypeptides that were detected by [S]methionine labelling. One of the induced proteins had a relative molecular mass ( ) of approx. 46000 and corresponded to l, as shown by immunoprecipitation with goat anti-reovirus antibody and a monoclonal antibody against l, The second induced protein had a of approx. 12000 and was very similar to s as judged by comparative tryptic peptide map analysis. Protein l produced in was shown to be functional as judged from its ability to bind to mouse L fibroblasts.

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1987-01-01
2024-03-29
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