Neuritic Transport of Herpes Simplex Virus in Rat Sensory Neurons in vitro. Effects of Substances Interacting with Microtubular Function and Axonal Flow [Nocodazole, Taxol and Erythro-9-3-(2-hydroxynonyl)adenine]
Herpes simplex virus type 1 and a fluorescein-labelled lectin (wheat germ agglutinin) were selectively transported to nerve cell bodies located in the inner compartment of a two-chamber tissue culture system after the application of virus or lectin to the neuritic processes in the outer culture compartment. Taxol, which stabilizes and alters intracellular arrangements of microtubules, and nocodazole, which disrupts microtubules, both inhibited this retrograde axonal transport of viral particles and lectin. The transport was also inhibited by erythro-9-3-(2-hydroxynonyl)adenine (EHNA), which blocks ATPases. However, EHNA was also an effective inhibitor of infection with the virus in non-neuronal cells (GMK AH-1). The nature of the action(s) of EHNA on neuritic transport of the virus is therefore less clear.
BattersonW.,
FurlongD.,
RoizmanB.1983; Molecular genetics of herpes simplex virus. VII. Further characterization of a ts mutant defective in release of viral DNA and in other stages of viral reproductive cycle. Journal of Virology 45:397–407
BouchardP.,
PenningrothS. M.,
CheungA.,
GagnonC.,
BardinC. W.1981; Erythro-9-3-(2-hydroxynonyl)adenine is an inhibitor of sperm motility that blocks dynein ATPase and protein carboxylmethylase activities. Proceedings of the National Academy of Sciences, U.S.A. 78:1033–1036
FormanD.,
BrownK. J.,
PromersbergerM. E.1983; Selective inhibition of retrograde axonal transport by erythro-9-3-(2-hydroxynonyl)adenine. Brain Research 272:194–197
GoldbergP. J.1982; Microinjection into an identified axon to study the mechanism of fast axonal transport. Proceedings of the National Academy of Sciences, U.S.A 79:4818–4822
GonatasN. K.1979; Immunochemistry and receptors: studies on the redistribution and adsorptive endocytosis of antiimmunoglobulin antibodies, cholera toxin, and lectins. Progress in Neuropathology 4:51–60
HermanB.,
AlbertiniD.1984; A time-lapse video image intensification analysis of cytoplasmic organelle movements during endosome translocation. Journal of Cell Biology 98:565–576
HermanB.,
LangevinM. A.,
AlbertiniD. F.1983; The effects of taxol on the organization of the cytoskeleton in cultured ovarian granulosa cells. European Journal of Cell Biology 31:34–45
LyckeE.,
KristenssonK.,
SvennerholmB.,
VahlneA.,
ZieglerR.1984; Uptake and transport of herpes simplex virus in neurites of rat dorsal root ganglia cells in culture. Journal of General Virology 65:55–64
MÅanssonJ. -E.,
OlofssonS.1983; Binding specificities of the lectins from Helix pomatia, soybean and peanut against different glycosphingolipids in liposome membranes. FEBS Letters 156:249–252
MasurovskyE. B.,
PetersonE. R.,
CrainS. M.,
HorwitzS. B.1981; Microtubule arrays in taxol-treated dorsal root ganglion-spinal cord cultures. Brain Research 217:392–398
SchiffP. B.,
HorwitzS. B.1980; Taxol stabilizes microtubules in mouse fibroblast cells. Proceedings of the National Academy of Sciences, U.S.A 77:1561–1565
SchnappB. J.,
ValeR. D.,
SheetyM. P.,
ReeseT. S.1985; Single microtubules from squid axoplasm support bidirectional movement of organelles. Cel l 40:455–462
ValeR. D.,
SchnappB. J.,
ReeseT. S.,
SheetyM. P.1985; Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon. Cel l 40:559–569
Neuritic Transport of Herpes Simplex Virus in Rat Sensory Neurons in vitro. Effects of Substances Interacting with Microtubular Function and Axonal Flow [Nocodazole, Taxol and Erythro-9-3-(2-hydroxynonyl)adenine]