RT Journal Article SR Electronic(1) A1 Czub, Markus A1 Braig, Henk Ronald A1 Diringer, HeinoYR 1986 T1 Pathogenesis of Scrapie: Study of the Temporal Development of Clinical Symptoms, of Infectivity Titres and Scrapie-associated Fibrils in Brains of Hamsters Infected Intraperitoneally JF Journal of General Virology, VO 67 IS 9 SP 2005 OP 2009 DO https://doi.org/10.1099/0022-1317-67-9-2005 PB Microbiology Society, SN 1465-2099, AB Summary After an intraperitoneal infection of hamsters with scrapie agent, early low and constant titres of about 100 LD50/brain between days 10 to 50 were followed by a dramatic increase to maximum levels of 3 × 109 LD50/brain within about 15 days. The plateau of maximum infectivity remained unchanged from day 70 to the time of the first and final signs of disease at 95 and 123 days post-infection, respectively. Scrapie-associated fibrils (SAF) as measured by immunoblotting of SAF protein could not be detected before 79 days post-infection even when a total brain was used for analysis. Subsequently, the concentration of SAF increased gradually by about 10000-fold until the time of clinical disease. The kinetics suggest a virus-induced amyloidosis of the brain as the cause of disease., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-9-2005