1887

Abstract

Summary

After an intraperitoneal infection of hamsters with scrapie agent, early low and constant titres of about 100 LD/brain between days 10 to 50 were followed by a dramatic increase to maximum levels of 3 × 10 LD/brain within about 15 days. The plateau of maximum infectivity remained unchanged from day 70 to the time of the first and final signs of disease at 95 and 123 days post-infection, respectively. Scrapie-associated fibrils (SAF) as measured by immunoblotting of SAF protein could not be detected before 79 days post-infection even when a total brain was used for analysis. Subsequently, the concentration of SAF increased gradually by about 10000-fold until the time of clinical disease. The kinetics suggest a virus-induced amyloidosis of the brain as the cause of disease.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-9-2005
1986-09-01
2024-07-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/9/JV0670092005.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-9-2005&mimeType=html&fmt=ahah

References

  1. Baringer J. R., Bowman K. A., Prusiner S. B. 1983; Replication of scrapie agent in hamster brain precedes neuronal vacuolation. Journal of Neuropathology and Experimental Neurology 42:539–547
    [Google Scholar]
  2. Bode L., Pocchiari M., Gelderblom H., Diringer H. 1985; Characterization of antisera against scrapie-associated fibrils (SAF) from affected hamster and cross-reactivity with SAF from scrapie-affected mice and from patients with Creutzfeldt-Jakob disease. Journal of General Virology 66:2471–2478
    [Google Scholar]
  3. Braig H. R., Diringer H. 1985; Scrapie: concept of a virus-induced amyloidosis of the brain. EMBO Journal 4:2309–2312
    [Google Scholar]
  4. Bruce M. E. 1981; Serial studies on the development of cerebral amyloidosis and vacuolar degeneration in murine scrapie. Journal of Comparative Pathology 91:589–597
    [Google Scholar]
  5. Bruce M. E. 1984; Scrapie and Alzheimer’s disease. Psychological Medicine 14:497–500
    [Google Scholar]
  6. Bruce M. E., Dickinson A. G., Fraser H. 1976; Cerebral amyloidosis in scrapie in the mouse: effect of agent strain and mouse genotype. Neuropathology and Applied Neurobiology 2:471–478
    [Google Scholar]
  7. Chesebro B., Rase R., Wehrly K., Nishio J., Bloom M., Lechner D., Bergstrom S., Robbins K., Mayer L., Keith J. M., Garon C., Haase A. 1985; Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain. Nature, London 315:331–333
    [Google Scholar]
  8. Collis S. C., Kimberlin R. H. 1985; Long-term persistence of scrapie infection on mouse spleens in the absence of clinical disease. FEMS Microbiology Letters 29:111–114
    [Google Scholar]
  9. Dickinson A. G., Meikle V. M. H. 1969; Genetical control of the concentration of ME7 scrapie agent in the brain of mice. Journal of Comparative Pathology 79:15–22
    [Google Scholar]
  10. Dickinson A. G., Outram G. W. 1979; The scrapie replication-site hypothesis and its implications for pathogenesis. In Slow Transmissible Diseases of the Nervous System vol 2 pp. 13–31 Edited by Prusiner S. B., Hadlow W. J. New York: Academic Press;
    [Google Scholar]
  11. Diringer H. 1984; Sustained viremia in experimental hamster scrapie. Archives of Virology 82:105–109
    [Google Scholar]
  12. Diringer H., Gelderblom H., Hilmert H., Ozel M., Edelbluth C., Kimberlin R. H. 1983a; Scrapie infectivity, fibrils and low molecular weight protein. Nature, London 306:476–478
    [Google Scholar]
  13. Diringer H., Hilmert H., Simon D., Werner W., Ehlers B. 1983b; Towards purification of the scrapie agent. European Journal of Biochemistry 134:555–560
    [Google Scholar]
  14. Eklund C. M., Kennedy R. C., Hadlow W. J. 1967; Pathogenesis of scrapie virus infection in the mouse. Journal of Infectious Diseases 117:15–22
    [Google Scholar]
  15. Fenner F. J. 1984; The pathogenesis and frequency of viral infections in man. In Viral Chemotherapy vol 1 pp. 27–50 Edited by Shugar D. Oxford: Pergamon Press;
    [Google Scholar]
  16. Fenner F. J., Mcauslan B. R., Mims C. A., Sambrook I., White D. O. 1974 The Biology of Animal Viruses pp. 452–453 New York: Academic Press;
    [Google Scholar]
  17. Gajdusek D. C. 1985; Unconventional viruses causing subacute spongiform encephalopathies. In Virology p 1519–1557 Edited by Fields B. N., Knipe D. M., Chanock R. M., Melnick J. L., Roizman B., Shope R. E. New York: Raven Press;
    [Google Scholar]
  18. Kimberlin R. H. 1976; Experimental scrapie in the mouse: a review of an important model disease. Science Progress, Oxford 63:461–481
    [Google Scholar]
  19. Kimberlin R. H., Walker C. A. 1977; Characteristics of a short incubation model of scrapie in the golden hamster. Journal of General Virology 34:295–304
    [Google Scholar]
  20. Kimberlin R. H., Walker C. A. 1979; Pathogenesis of mouse scrapie: dynamics of agent replication in spleen, spinal cord and brain after infection by different routes. Journal of Comparative Pathology 89:551–562
    [Google Scholar]
  21. Kimberlin R. K., Walker C. A. 1986; Pathogenesis of scrapie (strain 263K) in hamsters infected intracerebrally, intraperitoneally or intraocularly. Journal of General Virology 67:255–263
    [Google Scholar]
  22. Marsh R. F., Janson R. P. 1978; The Syrian hamster as a model for the study of slow virus diseases caused by unconventional agents. Federation Proceedings 37:2076–2078
    [Google Scholar]
  23. Merz P. A., Somerville R. A., Wisniewksi H. M., Iqbal K. 1981; Abnormal fibrils from scrapie-infected brain. Acta neuropathologica 54:63–74
    [Google Scholar]
  24. Merz P. A., Somerville R. A., Wisniewski H. M., Manuelidis L., Manuelidis E. E. 1983a; Scrapie associated fibrils in Creutzfeldt-Jakob disease. Nature, London 306:474–476
    [Google Scholar]
  25. Merz P. A., Somerville R. A., Wisniewski H. M. 1983b; Abnormal fibrils in scrapie and senile dementia of Alzheimer type. In Virus Non Conventionnels et Affections du Systeme Nerveux Central pp. 259–291 Edited by Court L. A., Cathala F. Paris: Masson;
    [Google Scholar]
  26. Moreau-dubois M. -C., Brown P., Rohwer R. G., Masters C. L., Franko M., Gajdusek D. C. 1982; Experimental scrapie in golden Syrian hamsters: temporal comparison of in vitro cell-fusing activity with brain infectivity and histopathological changes. Infection and Immunity 37:195–199
    [Google Scholar]
  27. Multhaup G., Diringer H., Hilmert H., Prinz H., Heukeshoven J., Beyreuther K. 1985; The protein component of scrapie-associated fibrils is a glycosylated low molecular weight protein. EMBO Journal 4:1495–1501
    [Google Scholar]
  28. Oesch B., Westaway D., Walchli M., Mckinley M. P., Kent S. B. H., Aebersold R., Barry R. A., Tempst P., Teplow D. B., Hood L. E., Prusiner S. B., Weissmann C. 1985; A cellular gene encodes scrapie PrP 27-30 protein. Cell 40:735–746
    [Google Scholar]
  29. Prusiner S. B., Cochran S. P., Downey D. E., Groth D. F. 1981; Determination of scrapie agent titer from incubation period measurements in hamsters. Proceedings of the Society for Experimental Biology and Medicine 134:385–399
    [Google Scholar]
  30. Prusiner S. B., Mckinley M. P., Bowman K. A., Bolton D. C., Bendheim P. E., Groth D. F., Glenner G. G. 1983; Scrapie prions aggregate to form amyloid-like birefringent rods. Cell 35:349–358
    [Google Scholar]
  31. Wisniewski H. M., Bruce M. E., Fraser H. 1975; Infectious etiology of neuritic (senile) plaques in mice. Science 1901108–1110
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-9-2005
Loading
/content/journal/jgv/10.1099/0022-1317-67-9-2005
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error