1887

Abstract

Summary

DNA from human T-lymphoid (Molt-4) and hamster kidney (BHK-21) cells infected with the T-lymphotropic simian foamy virus LK-3 was shown to be infectious, when assayed by transfection of BHK-21 cells. The proviral genome was further characterized by blot hybridization to a specific cDNA probe, which had been prepared by reverse transcription using viral RNA and RNA-dependent DNA polymerase present in cytoplasmic extracts of infected BHK-21 cells. This probe hybridized to a DNA species of 14 kbp in extracts from LK-3-infected diploid human fibroblasts, Molt-4 and BHK-21 cells, whereas no hybridization occurred with DNA from the respective uninfected controls. No integrated proviral DNA could be demonstrated, and the 14 kbp DNA was shown not to represent circular DNA. The patterns of restriction endonuclease and S1 nuclease fragments indicated a unique configuration of linear double-stranded DNA containing a single-stranded section separating two subunits one of which may be sufficient to transmit LK-3 by transfection with DNA.

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1986-09-01
2021-08-05
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References

  1. Achong B. G., Mansell P. W. A., Epstein M. A., Clifford P. 1971; An unusual virus in cultures from a human nasopharyngeal carcinoma. Journal of the National Cancer Institute 46:299–307
    [Google Scholar]
  2. Bender W., Chien Y.-H., Chattopadhyay S., Vogt P. K., Gardner M. B., Davidson N. 1978; High-molecular-weight RNAs of AKR, NZB, and wild mouse viruses and avian reticuloendotheliosis virus all have similar dimer structures. Journal of Virology 25:888–896
    [Google Scholar]
  3. Benzair A. -B., Rhodes-Feuillette A., Emanoil-Ravicovitch R., Peries J. 1983; Characterization of RNase H activity associated with reverse transcriptase in simian foamy virus type 1. Journal of Virology 47:249–252
    [Google Scholar]
  4. Chiswell D. J., Pringle C. R. 1977; Infectious DNA from cells infected with feline syncytium-forming virus (Spumavirinae). Journal of General Virology 36:551–555
    [Google Scholar]
  5. Chiswell D. I., Pringle C. R. 1978; Feline syncytium-forming virus proviral DNA. Time of synthesis and relationship to the host cell genome. Virology 90:344–350
    [Google Scholar]
  6. Chiswell D. J., Pringle C. R. 1979; Feline syncytium-forming virus: DNA provirus size and structure. Journal of General Virology 44:145–152
    [Google Scholar]
  7. Daniel M. D., King N. W., Letvin N. L., Hunt R. D., Sehgal P. K., Desrosiers R. C. 1984; A new type D retrovirus isolated from macaques with an immunodeficiency syndrome. Science 223:602–605
    [Google Scholar]
  8. Gardella T., Medveczky P., Sairenji T., Mulder C. 1984; Detection of circular and linear herpesvirus DNA molecules in mammalian cells by gel electrophoresis. Journal of Virology 50:248–254
    [Google Scholar]
  9. Gianni A. M., Weinberg R. A. 1975; Partially single-stranded form of free Moloney viral DNA. Nature, London 255:646–648
    [Google Scholar]
  10. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  11. Hooks J. J., Gibbs C. J. 1975; The foamy viruses. Bacteriological Reviews 39:169–185
    [Google Scholar]
  12. Hooks J. J., Hooks B. D. 1981; Spumavirinae: foamy virus group infections: comparative aspects and diagnosis. In Comparative Diagnosis of Viral Diseases vol 4 pp. 599–618 Edited by Kurstak E., Kurstak C. New York: Academic Press;
    [Google Scholar]
  13. Johnson P. H., Grossmann L. I. 1977; Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double-and single-stranded DNAs. Biochemistry 16:4217–4224
    [Google Scholar]
  14. Loh P. C., Matsuura F. S. 1981; The RNA of the human syncytium-forming (foamy) virus. Archives of Virology 68:53–58
    [Google Scholar]
  15. Neumann-Haefelin D., Rethwilm A., Bauer G., Gudat F., Zur Hausen H. 1983; Characterization of a foamy virus isolated from Cercopithecus aethiops lymphoblastoid cells. Medical Microbiology and Immunology 172:75–86
    [Google Scholar]
  16. Oliff A. I., Hager G. L., Chang E. H., Scolnick E. M., Chan H. W., Lowy D. R. 1980; Transfection of molecularly cloned Friend murine leukemia virus DNA yields a highly leukemogenic helper-independent type C virus. Journal of Virology 33:475–486
    [Google Scholar]
  17. Polack A., Delius H., Zimber U., Bornkamm G. W. 1984; Two deletions in the Epstein-Barr virus genome of the Burkitt lymphoma nonproducer line Raji. Virology 133:146–157
    [Google Scholar]
  18. Radloff R., Bauer W., Vinograd J. 1967; A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA of HeLa cells. Proceedings of the National Academy of Sciences, U.S.A. 57:1514–1521
    [Google Scholar]
  19. Varmus H., Swanstrom R. 1982; Replication of retroviruses. In RNA Tumor Viruses pp. 369–512 Edited by Weiss R., Teich N., Varmus H., Coffin J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Wigler M., Pellicer A., Silverstein S., Axel R. 1978; Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell 14:725–731
    [Google Scholar]
  21. Zur Hausen H., Gissmann L. 1979; Lymphotropic papovaviruses isolated from African green monkey and human cells. Medical Microbiology and Immunology 167:137–153
    [Google Scholar]
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