1887

Abstract

Summary

We have constructed a recombinant DNA library of the measles virus genome and identified gene-specific clones containing sequences coding for portions of each of the six viral structural proteins, as well as clones coding for intercistronic sequences. By Northern blotting, we could order the clones according to the pattern of individual gene-specific and readthrough mRNAs. Clones corresponding to the N, P/C and M genes were identified by correlation of the mRNAs with their translation products; clones corresponding to the H, F and L genes were identified by indirect evidence. The results indicated that the gene order in measles virus is that of a typical paramyxovirus (3′-N,P/C,M,F,H,L-5′), but that the M and F transcripts each contain 1.5 times the coding capacity needed for synthesis of these proteins .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-9-1987
1986-09-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/9/JV0670091987.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-9-1987&mimeType=html&fmt=ahah

References

  1. Alwine J. C., Kemp D. J., Stark G. R. 1977; Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proceedings of the National Academy of Sciences, U.S.A 74:5350–5354
    [Google Scholar]
  2. Bellini W. J., Silver G. D., Mcfarlln D. E. 1983; Biosynthesis of measles virus hemagglutinin in persistently infected cells. Archives of Virology 75:87–101
    [Google Scholar]
  3. Bellini W. J., Englund G., Rozenblatt S., Arnheiter H., Richardson C. D. 1985; Measles virus P gene codes for two proteins. Journal of Virology 53:908–919
    [Google Scholar]
  4. Billeter M. A., Baczko K., Schmid A., Ter Meulen V. 1984; Cloning of DNA corresponding to four different measles virus genomic regions. Virology 132:147–159
    [Google Scholar]
  5. Blumberg B. M., Giorgi C., Rose K., Kolakofsky D. 1985a; Sequence determination of the Sendai virus fusion protein gene. Journal of General Virology 66:317–331
    [Google Scholar]
  6. Blumberg B., Giorgi C., Roux L., Raju R., Dowling P., Chollet A., Kolakofsky D. 1985b; Sequence determination of the Sendai virus HN gene and its comparison to the influenza virus glycoproteins. Cell 41:269–278
    [Google Scholar]
  7. Dowling P. C., Giorgi C., Roux L., Dethlefsen L. A., Galantowicz M. E., Blumberg B. M., Kolakofsky D. 1983; Molecular cloning of the 3′ proximal third of Sendai virus genome. Proceedings of the National Academy of Sciences, U.S.A. 80:5213–5216
    [Google Scholar]
  8. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences, U.S.A. 72:3961–3965
    [Google Scholar]
  9. Kingsbury D. W. 1974; The molecular biology of paramyxoviruses. Medical Microbiology and Immunology 160:73–83
    [Google Scholar]
  10. Morgan E. M., Rapp F. 1977; Measles virus and its associated diseases. Bacteriological Reviews 41:636–666
    [Google Scholar]
  11. Richardson C. D., Berkovich A., Rozenblatt S., Bellini W. J. 1985; Use of antibodies directed against synthetic peptides for identifying cDNA clones, establishing reading frames, and deducing the gene order of measles virus. Journal of Virology 54:186–193
    [Google Scholar]
  12. Rima B. K. 1983; The proteins of morbilliviruses. Journal of General Virology 64:1205–1219
    [Google Scholar]
  13. Rozenblatt S., Eizenberg O., Englund G., Bellini W. J. 1985; Cloning and characterization of DNA complementary to the canine distemper virus mRNA encoding matrix, phosphoprotein, and nucleoprotein. Journal of Virology 53:691–694
    [Google Scholar]
  14. Shioda T., Hidaka Y., Kanda T., Shibuta H., Nomoto A., Iwasaki K. 1983; Sequence of 3, 687 nucleotides from the 3′ end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins. Nucleic Acids Research 11:7317–7330
    [Google Scholar]
  15. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Research 14:1545–1563
    [Google Scholar]
  16. Udem S. A., Cook K. A. 1984; Isolation and characterization of measles virus intracellular nucleocapsid RNA. Journal of Virology 49:57–65
    [Google Scholar]
  17. Walter P., Gilmore R., Blobel G. 1984; Protein translocation across the endoplasmic reticulum. Cell 385–8
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-9-1987
Loading
/content/journal/jgv/10.1099/0022-1317-67-9-1987
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error