1887

Abstract

Summary

cDNA clones of the largest RNA transcript of the canine distemper and measles morbilliviruses were characterized. This presumably codes for the L protein of these viruses. mRNA 4 was identified as coding for the haemagglutinin protein of measles virus. From an analysis of readthrough transcripts representing tandem copies of two or three genes we established a transcriptional map and the gene order on the negative strand genome of the morbilliviruses to be 3′-N-P+C-M-F-H-L-5′. The data exclude the presence of small intervening genes between the six major genes of morbilliviruses and indicate the gene order to be similar to that of Sendai virus and different from that of simian virus 5.

Keyword(s): CDV , gene order and measles virus
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/content/journal/jgv/10.1099/0022-1317-67-9-1971
1986-09-01
2022-09-24
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References

  1. Appel M. J. G., Gibbs E. P. J., Martin S. J., Ter Meulen V., Rima B. K., Stephenson J. R., Taylor W. P. 1981; Morbillivirus diseases of animals and man. In Comparative Diagnosis of Viral Diseases vol. 4 pp. 235–297 Edited by Kurstak E., Kurstak C. New York: Academic Press;
    [Google Scholar]
  2. Baczko K., Liebert U. G., Billeter M. A., Cattaneo R., Budka H., Ter Meulen V. 1986; Defective gene expression of measles virus in subacute sclerosing panencephalitis. Journal of Virology (in press)
    [Google Scholar]
  3. Barrett T., Mahy B. W. J. 1984; Molecular cloning of the nucleoprotein gene of canine distemper virus. Journal of General Virology 65:549–557
    [Google Scholar]
  4. Barrett T., Underwood B. 1985; Comparison of messenger RNAs induced in cells infected with each member of the morbillivirus group. Virology 145:195–199
    [Google Scholar]
  5. Barrett T., Shrimpton S. B., Russell S. E. H. 1985; Nucleotide sequence of the entire protein coding region of canine distemper virus polymerase-associated (P) protein mRNA. Virus Research 3:367–372
    [Google Scholar]
  6. Bellini W. J., Englund G., Rozenblatt S., Arnheiter H., Richardson C. D. 1985; Measles virus P gene codes for two proteins. Journal of Virology 53:908–919
    [Google Scholar]
  7. Bellini W. J., Englund G., Richardson C. D., Rozenblatt S., Lazzarini R. A. 1986; The matrix genes of measles virus and canine distemper virus: cloning, nucleotide sequence and deduced amino acid sequences. Journal of Virology 58:408–416
    [Google Scholar]
  8. Billeter M. A., Baczko K., Schmid A., Ter Meulen V. 1984; Cloning of DNA corresponding to four different measles virus genomic regions. Virology 132:147–159
    [Google Scholar]
  9. Chambers P., Millar N. S., Bingham R. W., Emmerson P. T. 1986; Molecular cloning of complementary DNA to Newcastle disease virus, and nucleotide sequence analysis of the junction between the genes encoding the haemagglutinin-neuraminidase and the large protein. Journal of General Virology 67:475–486
    [Google Scholar]
  10. Collins P. L., Wertz G. W. 1983; cDNA cloning and transcriptional mapping of nine polyadenylated RNAs encoded by the genome of human respiratory syncytial virus. Proceedings of the National Academy of Sciences, U.S.A 80:3208–3212
    [Google Scholar]
  11. Dowling P. C., Giorgi C., Roux L., Dethlefsen L. A., Galantovics M. E., Blumberg B. M., Kolakofsky D. 1983; Molecular cloning of the 3′ proximal third of the Sendai virus genome. Proceedings of the National Academy of Sciences, U.S.A. 80:5213–5216
    [Google Scholar]
  12. Feinberg A. P., Vogelstein B. 1984; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137:266–269
    [Google Scholar]
  13. Gorecki M., Rozenblatt S. 1980; Cloning of DNA complementary to the measles virus mRNA encoding nucleocapsid protein. Proceedings of the National Academy of Sciences, U.S.A 77:3686–3690
    [Google Scholar]
  14. Gupta K. C., Kingsbury D. W. 1985; Polytranscripts of Sendai virus do not contain intervening polyadenylate sequences. Virology 141:102–109
    [Google Scholar]
  15. Hall W. W., Kiesling W., Ter Meulen V. 1978; Membrane proteins of subacute sclerosing panencephalitis and measles virus. Nature, London 212:460–462
    [Google Scholar]
  16. Hermann R. C., Schubert M., Keene J. D., Lazzarini R. A. 1980; Polycistronic vesicular stomatitis virus RNA transcripts. Proceedings of the National Academy of Sciences, U.S.A 77:4662–4665
    [Google Scholar]
  17. Hiebert S. W., Paterson R. G., Lamb R. A. 1985; Identification and predicted sequence of a previously unrecognized small hydrophobic protein, SH, of the paramyxovirus simian virus 5. Journal of Virology 55:744–751
    [Google Scholar]
  18. Masters P. S., Samuel C. E. 1984; Detection of in vivo synthesis of polycistronic mRNAs of vesicular stomatitis virus. Virology 134:277–286
    [Google Scholar]
  19. Paterson R. G., Harris T. I. R., Lamb R. A. 1984; Analysis and gene assignment of mRNAs of a paramyxovirus, simian virus 5. Virology 138:310–323
    [Google Scholar]
  20. Richardson C. D., Berkovich A., Rozenblatt S., Bellini W. J. 1985; Use of antibodies directed against specific peptides for identifying cDNA clones, establishing reading frames and deducing the gene order of measles virus. Journal of Virology 54:186–193
    [Google Scholar]
  21. Rima B. K. 1983; The proteins of morbilliviruses. Journal of General Virology 64:1205–1219
    [Google Scholar]
  22. Rozenblatt S., Gesang C., Lavie V., Neumann F. S. 1982; Cloning and characterisation of DNA complementary to the measles virus mRNA encoding the hemagglutinin and matrix protein. Journal of Virology 42:790–797
    [Google Scholar]
  23. Rozenblatt S., Eizenberg O., Ben-Levy R., Lavie V., Bellini W. J. 1985a; Sequence homology within the morbilliviruses. Journal of Virology 53:684–690
    [Google Scholar]
  24. Rozenblatt S., Eizenberg O., Englund G., Bellini W. J. 1985b; Cloning and characterisation of DNA complementary to the canine distemper virus mRNA encoding matrix, phospho and nucleocapsid protein. Journal of Virology 53:691–694
    [Google Scholar]
  25. Russell S. E. H., Clarke D. K., Hoey E. M., Rima B. K., Martin S. J. 1985; cDNA cloning of the messenger RNAs of five genes of canine distemper virus. Journal of General Virology 66:977–983
    [Google Scholar]
  26. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Research 14:1545–1563
    [Google Scholar]
  27. Ter Meulen V., Carter M. J. 1982; Morbillivirus persistent infections in animals and man. In Virus Persistence pp. 97–132 Edited by Mahy B. W. J., Minson A. C., Darby G. K. Cambridge: Cambridge University Press;
    [Google Scholar]
  28. Udem S. A., Cook K. A. 1984; Isolation and characterisation of measles virus intracellular nucleocapsid RNA. Journal of Virology 49:57–65
    [Google Scholar]
  29. Wilde A., Morrison T. 1984; Structure and functional description of Newcastle disease virus polycistronic RNA species. Journal of Virology 51:71–76
    [Google Scholar]
  30. Yoshikawa Y., Yamanouchi K. 1984; Effect of papaverine treatment on replication of measles virus in human neural and nonneural cells. Journal of Virology 50:489–496
    [Google Scholar]
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