Nucleic acid hybridization techniques were used to analyse the fate of virus (CsV) DNAs in naturally parasitized and virus-injected larvae. Viral DNA persisted in injected larvae from 0 to 10 days post-injection but no increase in the amount of viral DNA could be detected. Similarly, no increase in the amount of viral DNA was detected in naturally parasitized larvae before the development of the pupae. However, a dramatic increase of viral DNA was detected in pharate and newly emerged (0 to 48 h) adult wasps. The results from these molecular analyses suggest that CsV replication does not occur in naturally parasitized or virus-injected and that viral replication may be restricted to tissues of wasps.


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