1887

Abstract

Summary

The inner capsid structure of the OSU strain of porcine rotavirus was studied by electron microscopy of freeze-dried preparations and of negatively stained chemically disrupted virus particles. The analysis of the particles by the freeze-drying technique revealed a T:13 () symmetry for the organization of the inner capsid. Treatment of single-capsid rotavirus particles with 30% formamide or 5 -urea resulted in their degradation, giving rise to very similar products, corresponding to isolated vertices, edges and faces of the virus icosahedron. An analysis of such structures confirmed the triangulation number and handedness of the rotavirus inner capsid, and provided evidence for the open-mesh model, in which the five- and six-coordinated axes are represented by ‘holes’ formed by smaller trimeric morphological subunits.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-8-1721
1986-08-01
2024-11-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/8/JV0670081721.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-8-1721&mimeType=html&fmt=ahah

References

  1. Almeida J., Bradburne A. F. S, Wreghitt T. G. 1979; The effect of sodium thiocyanate on virus structure. Journal of Medical Virology 4:269–277
    [Google Scholar]
  2. Bican P., Cohen J., Charpilienne A., Scherrer R. 1982; Purification and characterization of bovine rotavirus cores. Journal of Virology 43:1113–1117
    [Google Scholar]
  3. Esparza J., Gil F. 1978; A study on the ultrastructure of human rotavirus. Virology 91:141–150
    [Google Scholar]
  4. Esparza J., Gorziglia M., Liprandi F. 1984; Studies on the molecular biology of rotaviruses: a review. In Proceedings of the Fourth International Symposium on Neonatal Diarrhoea pp 1–26 Edited by Acres S. D. Saskatoon: Veterinary Infectious Diseases Organization;
    [Google Scholar]
  5. Gorziglia M., Larrea C., Liprandi F., Esparza J. 1985; Biochemical evidence for the oligomeric (possibly trimeric) structure of the major inner capsid polypeptide (45K) of rotaviruses. Journal of General Virology 66:1889–1900
    [Google Scholar]
  6. Klug A., Finch J. T. 1965; Structure of viruses of the papilloma-polyoma type. I. Human wart virus. Journal of Molecular Biology 11:403–423
    [Google Scholar]
  7. Kogasaka R., Akihara M., Horino K., Chiba S., Nakao T. 1979; A morphological study of human rotavirus. Archives of Virology 61:41–48
    [Google Scholar]
  8. Mccombs R. M., Williams G. A. 1973; Disruption of herpes virus nucleocapsids using lithium iodide, guanidine and mercaptoethanol. Journal of General Virology 20:395–400
    [Google Scholar]
  9. Martin M. L., Palmer E. L., Middleton P. I. 1975; Ultrastructure of infantile gastroenteritis virus. Virology 68:146–153
    [Google Scholar]
  10. Metcalf P. 1982; The symmetry of the reovirus outer shell. Journal of Ultrastructure Research 78:292–301
    [Google Scholar]
  11. Neurath A. R., Rubin B. A., Stasny J. T. 1968; Cleavage by formamide of intercapsomer bonds in adenovirus types 4 and 7 virions and hemagglutinins. Journal of Virology 2:1086–1095
    [Google Scholar]
  12. Novo E., Esparza J. 1981; Composition and topography of structural polypeptides of bovine rotavirus. Journal of General Virology 56:325–335
    [Google Scholar]
  13. Ӧzel M., Gelderblom H. 1985; Capsid symmetry of viruses of the proposed Bimavirus group. Archives of Virology 84:149–161
    [Google Scholar]
  14. Palmer E. L., Martin M. L. 1982; Further observations on the ultrastructure of human rotavirus. Journal of General Virology 62:105–111
    [Google Scholar]
  15. Palmer E. L., Martin M. L., Gary W. 1975; The ultrastructure of disrupted herpesvirus nucleocapsids. Virology 65:260–265
    [Google Scholar]
  16. Palmer E. L., Martin M. L., Murphy F. A. 1977; Morphology and stability of infantile gastroenteritis virus: comparison with reovirus and bluetongue virus. Journal of General Virology 35:403–414
    [Google Scholar]
  17. Roseto A., Escaig J., Delain E., Cohen J., Scherrer R. 1979; Structure of rotaviruses as studied by the freezedrying technique. Virology 98:471–475
    [Google Scholar]
  18. Stannard L. M., Schoub B. D. 1977; Observations on the morphology of two rotaviruses. Journal of General Virology 37:435–439
    [Google Scholar]
  19. Stasny J. T., Neurath A. R., Rubin B. A. 1968; Effect of formamide on the capsid morphology of adenovirus types 4 and 7. Journal of Virology 2:1429–1442
    [Google Scholar]
  20. Theil K. W., Bohl E. H., Agnes A. G. 1977; Cell culture propagation of porcine rotavirus (reovirus-like agent). American Journal of Veterinary Research 381765–1768
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-67-8-1721
Loading
/content/journal/jgv/10.1099/0022-1317-67-8-1721
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error