1887

Abstract

Summary

The stabilities of the antiviral states induced by different types of murine interferons (IFNs) were compared to gain insight into possible differences in the modes of regulation of their respective antiviral states. Both type I (MuIFN-α and MuIFN-β) and type II (MuIFN-γ) IFNs were employed to establish antiviral states against mengovirus in mouse L-929 cells. At various times after IFN treatment, the IFNs were removed and the stability of the antiviral states was determined by single cycle mengovirus yield reduction experiments. The antiviral states induced by the two type I IFNs decayed significantly by 12 h following IFN removal. The rate of this decay was an exponential function of the level of the antiviral state induced. A transcriptional block effectively delayed the decay of the antiviral state, suggesting the involvement of a positive feedback mechanism of regulation. In contrast, the profile of the antiviral state induced by type II IFN showed a significant enhancement upon MuIFN-γ removal. This enhancement was not dependent upon transcriptional and translational activity of the cells. These data suggest that the modes of regulation of the antiviral states against mengovirus induced by type I and type II IFNs are distinctly different.

Keyword(s): antiviral state , mengovirus and MuIFN
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-8-1645
1986-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/8/JV0670081645.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-8-1645&mimeType=html&fmt=ahah

References

  1. Auricchio F., Martin D. Jr, Tomkins G. 1969; Control of degradation and synthesis of induced tyrosine aminotransferase studied in hepatoma cells in culture. Nature, London 224:806–808
    [Google Scholar]
  2. Baron S., Dianzani F., Stanton G. J. 1982; General considerations of the interferon system. Texas Reports on Biology and Medicine 41:1–12
    [Google Scholar]
  3. Colonno R. J., Pang R. H. L. 1982; Induction of unique mRNAs by human interferons. Journal of Biological Chemistry 257:9234–9237
    [Google Scholar]
  4. Dianzani F., Zucca M., Scupham A., Georgiades J. A. 1980; Immune and virus-induced interferons may activate cells by different derepressional mechanisms. Nature, London 293:400–402
    [Google Scholar]
  5. Fleischmann W. R. Jr, Simon E. H. 1974; Mechanism of interferon induction by NDV: a monolayer and single cell study. Journal of General Virology 25:337–349
    [Google Scholar]
  6. Friedman R. L., Manly S. P., McMahon M., Kerr I. M., Stark G. R. 1984; Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells. Cell 38:745–755
    [Google Scholar]
  7. Hallum J. V., Thacore H. R., Youngner J. S. 1970; Factors affecting the sensitivity of different viruses to interferon. Journal of Virology 6:156–162
    [Google Scholar]
  8. McCormick W., Penman S. 1967; Inhibition of RNA synthesis in HeLa and L cells by mengovirus. Virology 31:135–141
    [Google Scholar]
  9. Masters P. S., Samuel C. E. 1983; Mechanism of interferon action: inhibition of vesicular stomatitis virus replication in human amnion U cells by cloned human leukocyte interferon. Journal of Biological Chemistry 258:12019–12033
    [Google Scholar]
  10. Osborne L. C., Georgiades J. A., Johnson H. M. 1979; Large scale production and partial purification of mouse immune interferon. Infection and Immunity 23:80–86
    [Google Scholar]
  11. Rose J. M., Crowley C. K., Fleischmann W. R. Jr 1985; Evidence that IFN-α/β induces two antiviral states active against different viruses. Journal of General Virology 66:1153–1158
    [Google Scholar]
  12. Ulker N., Samuel C. E. 1985; Mechanism of interferon action: inhibition of vesicular stomatitis virus replication in human amnion U cells by cloned human y-interferon. Journal of Biological Chemistry 260:4319–4330
    [Google Scholar]
  13. Weil J., Epstein C. J., Epstein L. B., Sedmak J. I., Sabran J. L., Grossberg S. E. 1983; A unique Set of polypeptides is induced by γ interferon in addition to those induced in common with α and β interferons. Nature, London 301:437–439
    [Google Scholar]
  14. West D. K., Ball L. A. 1982; Induction and maintenance of 2′, 5′-oligoadenylate synthetase in interferon-treated chicken embryo cells. Molecular and Cellular Biology 21436–1443
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-8-1645
Loading
/content/journal/jgv/10.1099/0022-1317-67-8-1645
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error