1887

Abstract

Summary

Two reassortant viruses were selected from a mixed infection of MA104 cells with human rotavirus strains Wa (serotype 1-subgroup II) and HN126 (serotype 2-subgroup I). Antigenic characterization and genotype analysis by polyacrylamide gel electrophoresis revealed that they were reassortants with novel antigenic compositions, i.e. serotype 1-subgroup I (C116) and serotype 2-subgroup II (C15). Furthermore one of them, C15, was considered to have a mosaic antigenicity defined by two serotype-specific antigens, namely the serotype 1-specific VP3 antigen and the serotype 2-specific VP7 antigen. Although this reassortant was shown to be a serotype 2 virus on the basis of its preferential reactivity in neutralization reactions with serotype 2 antiserum, unexpectedly the antiserum prepared against C15 equally neutralized both serotype 1 and serotype 2 strains.

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1986-08-01
2022-08-17
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References

  1. Beards G. M., Pilford J. N., Thouless M. E., Flewett T. H. 1980; Rotavirus serotypes by serum neutralization. Journal of Medical Virology 5:231–237
    [Google Scholar]
  2. Chenon A. G., Bricout F., Nicolas J. C. 1984; Study of genetic reassortment between two human rotaviruses. Virology 139:358–365
    [Google Scholar]
  3. Chiba Y., Miyazaki C., Makino Y., Mutanda L. N., Kibue A., Lichenga E., Tukei P. M. 1984; Rotavirus infection of young children in two districts of Kenya from 1982 to 1983 as analyzed by electrophoresis of genomic RNA. Journal of Clinical Microbiology 19:579–582
    [Google Scholar]
  4. Dyall-Smith M. L., Holmes I. H. 1981; Comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains. Journal of Virology 40:720–728
    [Google Scholar]
  5. Espejo R. T., Calderon E., Gonzalez N. 1977; Distinct reovirus-Iike agents associated with acute infantile gastroenteritis. Journal of Clinical Microbiology 6:502–506
    [Google Scholar]
  6. Espejo R. T., Calderon E., Gonzalez N., Salomon A., Martuscelli A., Romero P. 1979; Presence of two distinct types of rotavirus in infants and young children hospitalized with acute gastroenteritis in Mexico City, 1977. Journal of Infectious Diseases 139:474–477
    [Google Scholar]
  7. Espejo R. T., Munoz O., Serafin F., Romero P. 1980a; Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Infection and Immunity 27:351–354
    [Google Scholar]
  8. Espejo R. T., Avendano L. F., Munoz O., Romero P., Games J., Lopez S., Moncaya J. 1980b; Comparison of human rotaviruses isolated in Mexico City and in Santiago, Chile, by electrophoretic migration of their double-stranded ribonucleic acid genome segments. Infection and Immunity 30:342–348
    [Google Scholar]
  9. Flores J., Perez I., White L., Perez M., Kalica A. R., Marquina R., Wyatt R. G., Kapikian A. Z., Chanock R. M. 1982; Genetic relatedness among human rotaviruses as determined by RNA hybridization. Infection and Immunity 37:648–655
    [Google Scholar]
  10. Follett E. A. C., Desselberger U. 1983; Cocirculation of different rotavirus strains in a local outbreak of infantile gastroenteritis: monitoring by rapid and sensitive nucleic acid analysis. Journal of Medical Virology 11:39–52
    [Google Scholar]
  11. Gombold J. L., Ramig R. F. 1986; Analysis of reassortment of genome segments in mice mixedly infected with rotaviruses SA11 and RRV. Journal of Virology 57:110–116
    [Google Scholar]
  12. Greenberg H. B., Kalica A. R., Wyatt R. G., Jones R. W., Kapikian A. Z., Chanock R. M. 1981; Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proceedings of the National Academy of Sciences, U.S.A 78:420–424
    [Google Scholar]
  13. Greenberg H. B., Mcauliffe V., Valdesuso J., Wyatt R., Flores J., Kalica A., Hoshino Y., Singh N. 1983a; Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infection and Immunity 39:91–99
    [Google Scholar]
  14. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. 1983b; Gene coding assignments for growth restriction, neutralization and subgroup specificities of the Wa and DS-1 strains of human rotavirus. Journal of General Virology 64:313–320
    [Google Scholar]
  15. Greenberg H. B., Valdesuso J., Wyke K., Midthun K., Walsh M., Mcauliffe V., Wyatt R. G., Kalica A. R., Flores J., Hoshino Y. 1983c; Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. Journal of Virology 47:267–275
    [Google Scholar]
  16. Gunn P. R., Sato F., Powell K. F. H., Bellamy A. R., Napier J. R., Harding D. R. K., Hancock W. S., Siegman L. J., Both G. W. 1985; Rotavirus neutralization protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis. Journal of Virology 54:791–797
    [Google Scholar]
  17. Hoshino Y., Wyatt R. G., Flores J., Midthun K., Kapikian A. Z. 1985a; Serotype characterization of rotaviruses derived from asymptomatic human neonatal infections. Journal of Clinical Microbiology 21:425–430
    [Google Scholar]
  18. Hoshino Y., Sereno M. M., Saif L. J., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. 1985b; Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proceedings of the National Academy of Sciences, U.S.A. 82:8701–8704
    [Google Scholar]
  19. Kalica A. R., Sereno M. M., Wyatt R. G., Mebus C. A., Chanock R. M., Kapikian A. Z. 1978; Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87:247–255
    [Google Scholar]
  20. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. 1981a; Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112:385–390
    [Google Scholar]
  21. Kalica A. R., Greenberg H. B., Espejo R. T., Flores E. T., Wyatt R. G., Kapikian A. Z., Chanock R. M. 1981b; Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infection and Immunity 33:958–961
    [Google Scholar]
  22. Kalica A. R., Flores J., Greenberg H. B. 1983; Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation. Virology 125:194–205
    [Google Scholar]
  23. Lourenco M. H., Nicolas J. C., Cohen J., Scherrer R., Bricout F. 1981; Study of human rotavirus genome by electrophoresis attempt: of classification among strains isolated in France. Annales de Virologie 132E:161–173
    [Google Scholar]
  24. Mccrae M. A., Faulkner-Valle G. P. 1981; Molecular biology of rotaviruses. I. Characterization of basic growth parameters and pattern of macromolecular synthesis. Journal of Virology 39:490–496
    [Google Scholar]
  25. Mccrae M. A., Mccorquodale J. G. 1982; The molecular biology of rotaviruses. II. Identification of the proteincoding assignments of calf rotavirus genome RNA species. Virology 117:435–443
    [Google Scholar]
  26. Mason B. B., Graham D. Y., Estes M. K. 1983; Biochemical mapping of the simian rotavirus SA11 genome. Journal of Virology 46:413–423
    [Google Scholar]
  27. Matsuno S., Hasegawa A., Kalica A. R., Kono R. 1980; Isolation of a recombinant between simian and bovine rotaviruses. Journal of General Virology 48:253–256
    [Google Scholar]
  28. Nicolas J. C., Pothier P., Cohen J., Lourenco M. H., Thompson R., Guimbaud P., Chenon A., Dauvergne M., Bricout F. 1984; Survey of human rotavirus propagation as studied by electrophoresis of genomic RNA. Journal of Infectious Diseases 149:688–693
    [Google Scholar]
  29. Richardson M. A., Iwamoto A., Ikegami N., Nomoto A., Furuichi Y. 1984; Nucleotide sequence of the gene encoding the serotype-specific antigen of human (Wa) rotavirus: comparison with the homologous genes from simian SA11 and UK bovine rotaviruses. Journal of Virology 51:860–862
    [Google Scholar]
  30. Rodger S. M., Bishop R. F., Birch C., Mclean B., Holmes I. H. 1981; Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979 as determined by electrophoresis of genome ribonucleic acid. Journal of Clinical Microbiology 13:272–278
    [Google Scholar]
  31. Rodriguez W. J., Kim H. W., Brandt C. D., Gardner M. K., Parrott R. H. 1983; Use of electrophoresis of RNA from human rotavirus to establish the identity of strains involved in outbreaks in a tertiary care nursery. Journal of Infectious Diseases 148:34–40
    [Google Scholar]
  32. Smith M., Lazdins I., Holmes I. H. 1980; Coding assignments of double-stranded RNA segments of SA11 rotavirus established by in vitro translation. Journal of Virology 33:976–982
    [Google Scholar]
  33. Spencer E. G., Avendano L. F., Garcia B. I. 1983; Analysis of human rotavirus mixed electropherotypes. Infection and Immunity 39:569–574
    [Google Scholar]
  34. Street I. E., Croxson M. C., Chadderton W. F., Bellamy A. R. 1982; Sequence diversity of human rotavirus strains investigated by Northern blot hybridization analysis. Journal of Virology 43:369–378
    [Google Scholar]
  35. Taniguchi K., Urasawa S., Urasawa T. 1982; Electrophoretic analysis of RNA segments of human rotaviruses cultivated in cell culture. Journal of General Virology 60:171–175
    [Google Scholar]
  36. Taniguchi K., Urasawa T., Urasawa S., Yasuhara T. 1984; Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination. Journal of Medical Virology 14:115–125
    [Google Scholar]
  37. Taniguchi K., Urasawa S., Urasawa T. 1985; Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. Journal of General Virology 66:1045–1053
    [Google Scholar]
  38. Taniguchi K., Urasawa T., Urasawa S. 1986; Reactivity patterns to human rotavirus strains of a monoclonal antibody against VP2, a component of the inner capsid of rotavirus. Archives of Virology 87:135–141
    [Google Scholar]
  39. Urasawa S., Urasawa T., Taniguchi K. 1982; Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains. Infection and Immunity 38:781–784
    [Google Scholar]
  40. Urasawa S., Urasawa T., Taniguichi K., Chiba S. 1984; Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan. Archives of Virology 81:1–12
    [Google Scholar]
  41. Wyatt R. G., James H. D., Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. 1983; Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. Journal of Clinical Microbiology 18:310–317
    [Google Scholar]
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