1887

Abstract

Summary

Intracellular virus-specific proteins induced by equine arteritis virus (EAV) have been compared with translation products of virion and intracellular EAV RNAs. In infected BHK-21 cells, the two major virion proteins (C and E1) and polypeptides with mol. wt. of 60000 (p60), 42000 (p42) and 30000 (p30) were found. There were no indications that the viral proteins were processed from a larger precursor as shown by pulse-chase, amino acid analogue and protease inhibitor experiments. The six polyadenylated RNAs that occur in EAV-infected cells were isolated and translated in an mRNA-dependent reticulocyte cell-free system. Translation of RNA6 resulted in the appearance of a product having the mol. wt. (14000) of the nucleocapsid protein (C). EAV genomic RNA was translated into proteins of mol. wt. 30000 and 200000, while RNA1, the intracellular homologue of genomic RNA only encoded p30. The absence of large precursor molecules in infected cells and the results from the translation experiments both suggest that at least some of the proteins are primary translation products.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-8-1543
1986-08-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/8/JV0670081543.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-8-1543&mimeType=html&fmt=ahah

References

  1. Doll E. R., Bryans J. T., Mccollum W. H., Crowe M. E. W. 1957; Isolation of a filterable agent causing arteritis of horses and abortion by mares. Its differentiation from the equine abortion (influenza) virus. Cornell Veterinarian 47:3–41
    [Google Scholar]
  2. Guilley H., Carrington J. C., Balazs E., Richards K., Morris T. J. 1985; Nucleotide sequence and genome organisation of carnation mottle virus RNA. Nucleic Acids Research 13:6663–6677
    [Google Scholar]
  3. Horzinek M. C. 1981 Non-arthropod Borne Togaviruses London: Academic Press;
    [Google Scholar]
  4. Jones T. C., Doll E. R., Bryans J. T. 1957; The lesions of equine viral arteritis. Cornell Veterinarian 47:52–68
    [Google Scholar]
  5. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  6. Porterfield J. S., Casals J., Chumakov M. P., Gaidamovich S. Ya., Hannoun C., Holmes I. H., Horzinek M. C., Mussgay M., Oker-Blom N., Russell P. K., Trent D. W. 1978; Togaviridae. Intervirology 9:129–148
    [Google Scholar]
  7. Rottier P. J. M., Spaan W. J. M., Horzinek M. C., Van Der Zeijst B. A. M. 1981; Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes. Journal of Virology 38:20–26
    [Google Scholar]
  8. Van Berlo M. F., Horzinek M. C., Van Der Zeijst B. A. M. 1980; Temperature-sensitive mutants of equine arteritis virus. Journal of General Virology 49:97–104
    [Google Scholar]
  9. Van Berlo M. F., Horzinek M. C., Van Der Zeijst B. A. M. 1982; Equine arteritis virus-infected cells contain six polyadenylated virus-specific RNAs. Virology 118:345–352
    [Google Scholar]
  10. Van Der Zeijst B. A. M., Horzinek M. C., Moennig V. 1975; The genome of equine arteritis virus. Virology 68:418–425
    [Google Scholar]
  11. Westaway E. G. 1980; Replication of flaviviruses. In The Togaviruses pp 531–581 Edited by Schlesinger R. W. New York & London: Academic Press;
    [Google Scholar]
  12. Westaway E. G., Brinton M. A., Gaidamovich S. Ya., Horzinek M. C., Igarashi A., Kääriäinen L., Lvov D. K., Porterfield J. S., Russell P. K., Trent D. W. 1985; Togaviridae. Intervirology 24:125–139
    [Google Scholar]
  13. Zeegers J. J. W., Van Der Zeijst B. A. M., Horzinek M. C. 1976; The structural proteins of equine arteritis virus. Virology 73200–205
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-8-1543
Loading
/content/journal/jgv/10.1099/0022-1317-67-8-1543
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error