1887

Abstract

Summary

A virus-neutralizing monoclonal antibody (1E3) specifically immunoprecipitated the 70000 mol. wt. (70K) fusion (F) protein from respiratory syncytial (RS) virus-infected HeLa cells. Western blotting analysis of polypeptides from such cells separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions revealed that 1E3 was peculiar in that it bound to both F (50K) and F (20K) components of the F protein. Antibody subsequently eluted from either the F or the F regions of immunoblots re-bound to both F and F regions of the SDS-PAGE blot. These results show that monoclonal antibody 1E3 reacts with an epitope which is found on both F and F subunits of RS virus fusion protein.

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/content/journal/jgv/10.1099/0022-1317-67-7-1479
1986-07-01
2021-10-20
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References

  1. Burnette W. N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  2. Collins P. L., Huang Y. T., Wertz G. W. 1984; Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proceedings of the National Academy of Sciences, U.S.A. 81:7683–7687
    [Google Scholar]
  3. Elango N., Satake M., Coligan J. E., Norrby E., Camargo E., Venkatesan S. 1985; Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit. Nucleic Acids Research 13:1559–1574
    [Google Scholar]
  4. Kim H. W., Arrobio J. O., Brandt C. D., Jeffries B. C., Pyles G., Reid J. L., Chanock R. M., Parrott R. H. 1973; Epidemiology of respiratory syncytial infection in Washington, D.C. I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection. American Journal of Epidemiology 98:216–225
    [Google Scholar]
  5. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., Ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  7. Lambert D. M., Pons M. W. 1983; Respiratory syncytial virus glycoproteins. Virology 130:204–214
    [Google Scholar]
  8. Nagai Y., Klenk H. -D. 1977; Activation of precursors to both glycoproteins of Newcastle disease virus by proteolytic cleavage. Virology 77:125–134
    [Google Scholar]
  9. Queen C. L., Korn L. J. 1980; Computer analysis of nucleic acids and protein. Methods in Enzymology 65:595–609
    [Google Scholar]
  10. Routledge E. G., McQuillan J., Samson A. C. R., Toms G. L. 1985; The development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence. Journal of Medical Virology 15:305–320
    [Google Scholar]
  11. Samson A. C. R., Fox C. F. 1973; A precursor protein for Newcastle disease virus. Journal of Virology 12:579–587
    [Google Scholar]
  12. Talian J. C., Olmsted J. B., Goldman R. D. 1983; A rapid procedure for preparing fluorescein-labelled specific antibodies from whole antiserum: its use in analysing cytoskeletal architecture. Journal of Cell Biology 97:1277–1282
    [Google Scholar]
  13. Walsh E. E., Schlesinger J.J., Brandriss M. W. 1983; Protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. Infection and Immunity 43:756–758
    [Google Scholar]
  14. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1984; Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus. Journal of General Virology 65:761–767
    [Google Scholar]
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