The IgG subclass specificity of Fc receptor(s) induced on cells by infection with human cytomegalovirus (HCMV) was studied in a binding assay by using infected cells and purified iodinated IgG of various subclasses from HCMV seronegative healthy adult donors. All four human IgG subclasses bound to HCMV-infected cells, with the following relative magnitudes: IgG1 ⩾ IgG4 > IgG2 > IgG3. The IgG subclass specificity of the Fc receptor was further analysed in an inhibition assay by using fragments prepared from purified human IgG by papain digestion, and using unlabelled subclass proteins. Fc but not Fab fragments inhibited the binding of 125I-labelled human IgG to HCMV-infected cells. The biological role of the Fc receptor in HCMV infection is discussed.
AdlerR.,
GloriosoJ. C.,
CossmanJ.,
LevineM.1978; Possible role of Fc receptors on cells infected and transformed by herpesvirus: escape from immune cytolysis. Infection and Immunity 21:442–447
AndersonC. L.,
AbrahamO. N.1980; Characterization of the Fc receptor for IgG on a human macrophage cell line, U937. Journal of Immunology 125:2735–2741
BauckeR. B.,
SpearP. G.1979; Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc-binding glycoprotein. Journal of Virology 32:779–789
ByrtP.,
AdaG. L.1969; An in vitro reaction between labelled flagellin or haemocyamin and lymphocyte-like cells from normal animals. Immunology 17:503–516
FreyJ.,
EinsfelderB.1984; Induction of surface IgG receptors in cytomegalovirus-infected human fibroblasts. European Journal of Biochemistry 138:213–216
FurukawaT.,
FiorettiA.,
PlotkinS. A.1973; Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology 11:991–997
FurukawaT.,
HornbergerE.,
SakumaS.,
PlotkinS. A.1975; Demonstration of immunoglobulin G receptors induced by human cytomegalovirus. Journal of Clinical Microbiology 2:332–336
HjelmH.,
HjelmK.,
SjoquistI.1972; Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Letters 28:73–76
JohanssonP. J. H.,
HallbergT.,
OxeliusV. A.,
GrubbA.,
BlombergJ.1984; Human immunoglobulin class and subclass specificity of Fc receptors induced by herpes simplex virus type 1. Journal of Virology 50:796–804
KellerR.,
PeitchelR.,
GoldmanJ. N.,
GoldmanM.1976; An IgG-Fc receptor induced in cytomegalovirus-infected human fibroblasts. Journal of Immunology 116:772–777
LindeG. A.,
HammarstromL.,
PerssonM. A. A.,
SmithC. I. E.,
SundqvistV. A.,
WahrenB.1983; Virus-specific antibody activity of different subclasses of immunoglobulins G and A in cytomegalovirus infections. Infection and Immunity 42:237–244
LinnemannC. C.,
MayD. B.JR,
SchubertW. K.,
CarawayC. T.,
SchiffG. M.1973; Fetal viral encephalitis in children with X-linked hypogammaglobulinemia. American Journal of Diseases of Children 126:100–103
ParaM. F.,
BauckeR. B.,
SpearP. G.1982; Glycoprotein gE of herpes simplex virus type 1: effects of anti-gE on virion infectivity and on virus-induced Fc-binding receptors. Journal of Virology 41:129–136
RahmanA. A.,
TeschnerM.,
SethiK. K.,
BrandisH.1976; Appearance of IgG (Fc) receptor(s) on cultured human fibroblasts infected with human cytomegalovirus. Journal of Immunology 117:253–258
SakumaS.,
FurukawaT.,
PlotkinS. A.1977; The characterization of IgG receptor induced by human cytomegalovirus. Proceedings of the Society for Experimental Biology and Medicine 155:168–172
SchumakerV. N.,
CalcottM. A.,
SpiegelbergH. L.,
Muller-EberhardH. J.1976; Ultracentrifuge studies of the binding of IgG of different subclasses to the Clq subunit of the first component of complement. Biochemistry 15:5175–5181
StinskiM. F.1978; Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides. Journal of Virology 26:686–701
WestmorelandD.,
WatkinsJ. F.1974; The IgG receptor induced by herpes simplex virus: studies using radioiodinated IgG. Journal of General Virology 24:167–178
WestmorelandD.,
St JeorS.,
RappF.1976; The development by cytomegalovirus-infected cells of binding affinity for normal human immunoglobulin. Journal of Immunology 116:1566–1570