Fc Receptor(s) Induced by Human Cytomegalovirus Bind Differentially with Human Immunoglobulin G Subclasses Free

Abstract

Summary

The IgG subclass specificity of Fc receptor(s) induced on cells by infection with human cytomegalovirus (HCMV) was studied in a binding assay by using infected cells and purified iodinated IgG of various subclasses from HCMV seronegative healthy adult donors. All four human IgG subclasses bound to HCMV-infected cells, with the following relative magnitudes: IgG1 ⩾ IgG4 > IgG2 > IgG3. The IgG subclass specificity of the Fc receptor was further analysed in an inhibition assay by using fragments prepared from purified human IgG by papain digestion, and using unlabelled subclass proteins. Fc but not Fab fragments inhibited the binding of I-labelled human IgG to HCMV-infected cells. The biological role of the Fc receptor in HCMV infection is discussed.

Keyword(s): Fc receptor(s) , HCMV and IgG subclasses
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/content/journal/jgv/10.1099/0022-1317-67-7-1475
1986-07-01
2024-03-28
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References

  1. Adler R., Glorioso J. C., Cossman J., Levine M. 1978; Possible role of Fc receptors on cells infected and transformed by herpesvirus: escape from immune cytolysis. Infection and Immunity 21:442–447
    [Google Scholar]
  2. Anderson C. L., Abraham O. N. 1980; Characterization of the Fc receptor for IgG on a human macrophage cell line, U937. Journal of Immunology 125:2735–2741
    [Google Scholar]
  3. Baucke R. B., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc-binding glycoprotein. Journal of Virology 32:779–789
    [Google Scholar]
  4. Beck O. E. 1981; Distribution of virus antibody activity among human IgG subclasses. Clinical and Experimental Immunology 43:626–632
    [Google Scholar]
  5. Byrt P., Ada G. L. 1969; An in vitro reaction between labelled flagellin or haemocyamin and lymphocyte-like cells from normal animals. Immunology 17:503–516
    [Google Scholar]
  6. Frey J., Einsfelder B. 1984; Induction of surface IgG receptors in cytomegalovirus-infected human fibroblasts. European Journal of Biochemistry 138:213–216
    [Google Scholar]
  7. Furukawa T., Fioretti A., Plotkin S. A. 1973; Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology 11:991–997
    [Google Scholar]
  8. Furukawa T., Hornberger E., Sakuma S., Plotkin S. A. 1975; Demonstration of immunoglobulin G receptors induced by human cytomegalovirus. Journal of Clinical Microbiology 2:332–336
    [Google Scholar]
  9. Hjelm H., Hjelm K., Sjoquist I. 1972; Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Letters 28:73–76
    [Google Scholar]
  10. Johansson P. J. H., Hallberg T., Oxelius V. A., Grubb A., Blomberg J. 1984; Human immunoglobulin class and subclass specificity of Fc receptors induced by herpes simplex virus type 1. Journal of Virology 50:796–804
    [Google Scholar]
  11. Keller R., Peitchel R., Goldman J. N., Goldman M. 1976; An IgG-Fc receptor induced in cytomegalovirus-infected human fibroblasts. Journal of Immunology 116:772–777
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Linde G. A., Hammarstrom L., Persson M. A. A., Smith C. I. E., Sundqvist V. A., Wahren B. 1983; Virus-specific antibody activity of different subclasses of immunoglobulins G and A in cytomegalovirus infections. Infection and Immunity 42:237–244
    [Google Scholar]
  14. Linnemann C. C., May D. B. JR, Schubert W. K., Caraway C. T., Schiff G. M. 1973; Fetal viral encephalitis in children with X-linked hypogammaglobulinemia. American Journal of Diseases of Children 126:100–103
    [Google Scholar]
  15. Para M. F., Baucke R. B., Spear P. G. 1982; Glycoprotein gE of herpes simplex virus type 1: effects of anti-gE on virion infectivity and on virus-induced Fc-binding receptors. Journal of Virology 41:129–136
    [Google Scholar]
  16. Porter R. R. 1959; The hydrolysis of rabbit gammaglobulin and antibodies with crystalline papain. Biochemical Journal 72:119–126
    [Google Scholar]
  17. Rahman A. A., Teschner M., Sethi K. K., Brandis H. 1976; Appearance of IgG (Fc) receptor(s) on cultured human fibroblasts infected with human cytomegalovirus. Journal of Immunology 117:253–258
    [Google Scholar]
  18. Sakuma S., Furukawa T., Plotkin S. A. 1977; The characterization of IgG receptor induced by human cytomegalovirus. Proceedings of the Society for Experimental Biology and Medicine 155:168–172
    [Google Scholar]
  19. Schumaker V. N., Calcott M. A., Spiegelberg H. L., Muller-Eberhard H. J. 1976; Ultracentrifuge studies of the binding of IgG of different subclasses to the Clq subunit of the first component of complement. Biochemistry 15:5175–5181
    [Google Scholar]
  20. Stinski M. F. 1978; Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides. Journal of Virology 26:686–701
    [Google Scholar]
  21. Unkeless J. C., Fleit H., Mellman I. S. 1981; Structural aspects and heterogeneity of immunoglobulin Fc receptors. Advances in Immunology 31:247–270
    [Google Scholar]
  22. Watkins J. F. 1964; Adsorption of sensitized sheep erythrocytes to HeLa cells infected with herpes simplex virus. Nature, London 202:1364–1365
    [Google Scholar]
  23. Westmoreland D., Watkins J. F. 1974; The IgG receptor induced by herpes simplex virus: studies using radioiodinated IgG. Journal of General Virology 24:167–178
    [Google Scholar]
  24. Westmoreland D., St Jeor S., Rapp F. 1976; The development by cytomegalovirus-infected cells of binding affinity for normal human immunoglobulin. Journal of Immunology 116:1566–1570
    [Google Scholar]
  25. Wright P. F., Hatch M. H., Kasselberg A. G., Lowry S. P., Wadlington W. B., Karzon D. T. 1977; Vaccine-associated poliomyelitis in a child with sex-linked agammaglobulinemia. Journal of Pediatrics 91:408–412
    [Google Scholar]
  26. Wyatt H. V. 1973; Poliomyelitis in hypogammaglobulinemics. Journal of Infectious Diseases 128:802–806
    [Google Scholar]
  27. Yasuda J., Milgram F. 1968; Hemadsorption by herpes simplex-infected cell cultures. International Archives of Allergy and Applied Immunology 33:151–170
    [Google Scholar]
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