RT Journal Article SR Electronic(1) A1 Mach, Michael A1 Utz, Ursula A1 Fleckenstein, BernhardYR 1986 T1 Mapping of the Major Glycoprotein Gene of Human Cytomegalovirus JF Journal of General Virology, VO 67 IS 7 SP 1461 OP 1467 DO https://doi.org/10.1099/0022-1317-67-7-1461 PB Microbiology Society, SN 1465-2099, AB Summary The gene coding for the most abundant glycoprotein (gp58) of human cytomegalovirus (HCMV), strain AD169, was physically mapped on the viral genome. A monospecific rabbit antiserum against gp58 was used to screen a cDNA library that was constructed from poly(A)+ RNA of HCMV-infected cells in the prokaryotic expression vector λ gt11. A cDNA clone was identified which synthesized part of the glycoprotein. It allowed localization of the coding region within the right terminal sequence of the HindIII-F fragment between map coordinates 0.344 and 0.380 of HCMV virion DNA., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-7-1461