%0 Journal Article %A Mach, Michael %A Utz, Ursula %A Fleckenstein, Bernhard %T Mapping of the Major Glycoprotein Gene of Human Cytomegalovirus %D 1986 %J Journal of General Virology, %V 67 %N 7 %P 1461-1467 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-67-7-1461 %K glycoprotein %K HCMV %K gene mapping %I Microbiology Society, %X Summary The gene coding for the most abundant glycoprotein (gp58) of human cytomegalovirus (HCMV), strain AD169, was physically mapped on the viral genome. A monospecific rabbit antiserum against gp58 was used to screen a cDNA library that was constructed from poly(A)+ RNA of HCMV-infected cells in the prokaryotic expression vector λ gt11. A cDNA clone was identified which synthesized part of the glycoprotein. It allowed localization of the coding region within the right terminal sequence of the HindIII-F fragment between map coordinates 0.344 and 0.380 of HCMV virion DNA. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-7-1461