1887

Abstract

Summary

The gene coding for the most abundant glycoprotein (gp58) of human cytomegalovirus (HCMV), strain AD169, was physically mapped on the viral genome. A monospecific rabbit antiserum against gp58 was used to screen a cDNA library that was constructed from poly(A) RNA of HCMV-infected cells in the prokaryotic expression vector λ gt11. A cDNA clone was identified which synthesized part of the glycoprotein. It allowed localization of the coding region within the right terminal sequence of the dIII-F fragment between map coordinates 0.344 and 0.380 of HCMV virion DNA.

Keyword(s): gene mapping , glycoprotein and HCMV
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/content/journal/jgv/10.1099/0022-1317-67-7-1461
1986-07-01
2022-01-20
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