1887

Abstract

Summary

Tissues of human primary hepatocellular carcinoma (PHC) from six patients infected with hepatitis B virus (HBV) were propagated in nude mice, as well as a strain of hepatitis B surface antigen-positive PHC (PLC/PRF/5). Integration of viral DNA into chromosomal DNA of tumour cells was evaluated by the capacity to hybridize with radiolabelled DNA probes, each representing fundamental partsof the HBV genome, that is S and C genes and regions pre-S and X. All PHC cells possessed region X integrated in their chromosomes. However, integration of the S gene, C gene and region pre-S was found in only six of the seven PHCs. Based on these findings, the integration of region X seems to be most closely associated with carcinogenesis in HBV infection.

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/content/journal/jgv/10.1099/0022-1317-67-7-1449
1986-07-01
2021-10-16
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References

  1. Alexander J. J., Bey E. M., Geddes E. W., Lacatsas G. 1976; Establishment of a continuously growing cell-line from primary carcinoma of the liver. South African Medical Journal 50:2124–2128
    [Google Scholar]
  2. Beasley R. P., Hwang L., Lin C., Chien C. 1981; Hepatocellular carcinoma and hepatitis B virus: a prospective study of 22, 707 men in Taiwan. Lancet ii:1129–1133
    [Google Scholar]
  3. Brechot C., Pourcel C., Louise A., Rain B., Tiollais P. 1980; Presence of integrated hepatitis B virus DNA sequences in cellular DNA of human hepatocellular carcinoma. Nature, London 286:533–535
    [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., Macdonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  5. Dejean A., Sonigo P., Wain-Hobson S., Tiollais P. 1984; Specific hepatitis B virus integration in hepatocellular carcinoma DNA through a viral 11-base-pair direct repeat. Proceedings of the National Academy of Sciences, U.S.A. 81:5350–5354
    [Google Scholar]
  6. Edman J. C., Gray P., Valenzuela P., Rall L. B., Rutter W. J. 1980; Integration of hepatitis B virus sequences and their expression in a human hepatoma cell. Nature, London 286:535–538
    [Google Scholar]
  7. Kam W., Rall L. B., Smuckler E. A., Schmid R., Rutter W. J. 1982; Hepatitis B viral DNA in liver and serum of asymptomatic carriers. Proceedings of the National Academy of Sciences, U.S.A. 79:7522–7526
    [Google Scholar]
  8. Moriarty A. M., Alexander H., Lerner R. A. 1985; Antibodies to peptides detect new hepatitis B antigen: serological correlation with hepatocellular carcinoma. Science 227:429–433
    [Google Scholar]
  9. Ono Y., Onda H., Sasada R., Igarashi K., Sugino Y., Nishioka K. 1983; The complete nucleotide sequences of the cloned hepatitis B virus DNA; subtype adr and adw. Nucleic Acids Research 11:1747–1757
    [Google Scholar]
  10. Perucho M., Goldfarb M., Shimizu K., Lama C., Fogh J., Wigler M. 1981; Human-tumor-derived cell lines contain common and different transforming genes. Cell 27:467–476
    [Google Scholar]
  11. Rigby P. W. J., Dieckmann M., Rhodes C., Erg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  12. Shafritz D. A., Shouval D., Sherman H. I., Hadziyannis S. J., Kewm C. 1981; Integration of hepatitis B virus DNA into the genome of liver cells in chronic liver disease and hepatocellular carcinoma: studies in percutaneous liver biopsies and post-mortem tissue specimens. New England Journal of Medicine 305:1067–1073
    [Google Scholar]
  13. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  14. Szmuness W. 1978; Hepatocellular carcinoma and hepatitis B virus: evidence fora causal association. Progress in Medical Virology 24:40–65
    [Google Scholar]
  15. Takahashi K., Akahane Y., Gotanda Y., Mishiro S., Imai M., Miyakawa Y., Mayumi M. 1979; Demonstration of hepatitis B e antigen in the core of Dane particles. Journal of Immunology 122:275–279
    [Google Scholar]
  16. Tiollais P., Charnay P., Vyas G. N. 1981; Biology of hepatitis B virus. Science 213:406–411
    [Google Scholar]
  17. Ziemer M., Garcia P., Shaul Y., Rutter W. J. 1985; Sequence of hepatitis B virus DNA incorporated into the genome of a human hepatoma cell line. Journal of Virology 53:885–892
    [Google Scholar]
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