1887

Abstract

Summary

Previously, a panel of monoclonal antibodies recognizing epitopes in four antigenic sites on the haemagglutinin—neuraminidase (HN) glycoprotein of the Australia-Victoria strain of Newcastle disease virus were used in strain comparisons. Epitopes in three sites were found to be conserved while the epitope recognized by the single antibody to site 3 was not. A new panel of antibodies is described, two of which bind to epitopes in site 3 and six of which bind to a site (site 1,4) that overlaps with sites 1 and 4 as determined by analyses of variants, temperature-sensitive mutants, and strains by assays of neutralization of infectivity and binding in a radioimmunoassay. Neutralization of heterologous strains with the panel of antibodies revealed that both new site 3 epitopes are also highly divergent, while three additional epitopes outside site 3 (those in site 1,4) are highly conserved. The new site 3 antibodies can bind to virions of several heterologous strains without neutralizing infectivity. Thus, of the 10 epitopes we have now examined, all of three in site 3 are specific with respect to neutralization of infectivity for th ehomologous strain, while all of seven in other sites are conserved in heterologous strains. This suggests that the strain specificity originally described for a single site 3 epitope is, instead, a property of a much more extensive, poorly conserved domain on the HN molecule.

Keyword(s): HN , monoclonal antibodies and NDV
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/content/journal/jgv/10.1099/0022-1317-67-7-1393
1986-07-01
2021-10-15
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References

  1. Avery R. G., Niven J. 1979; Use of antibodies to purified Newcastle disease virus glycoproteins for strain comparisons and characterizations. Infection and Immunity 26:795–801
    [Google Scholar]
  2. Bratt M. A., Gallaher W. R. 1969; Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proceedings of the National Academy of Sciences, U.S.A. 64:536–543
    [Google Scholar]
  3. Chatis P. A., Morrison T. G. 1981; Mutational changes in the vesicular stomatitis virus glycoprotein affect the requirement of carbohydrate in morphogenesis. Journal of Virology 37:307–316
    [Google Scholar]
  4. Clavell L. A., Bratt M. A. 1972; Hemolytic interaction of Newcastle disease virus and chicken erythrocytes. II. Determining factors. Applied Microbiology 23:461–470
    [Google Scholar]
  5. Hightower L. E., Bratt M. A. 1974; Protein synthesis in Newcastle disease virus-infected chicken embryo cells. Journal of Virology 13:788–800
    [Google Scholar]
  6. Iorio R. M., Bratt M. A. 1983; Monoclonal antibodies to Newcastle disease virus: delineation of four epitopes on the HN glycoprotein. Journal of Virology 48:440–450
    [Google Scholar]
  7. Iorio R. M., Bratt M. A. 1984a; Four functional domains on the HN glycoprotein of Newcastle disease virus. In Nonsegmented Negative Strand Viruses pp 309–314 Edited by Bishop D. H. L., Compans R. W. New York: Academic Press;
    [Google Scholar]
  8. Iorio R. M., Bratt M. A. 1984b; Neutralization of Newcastle disease virus by monoclonal antibodies to the HN glycoprotein: requirement for antibodies to four sites for complete neutralization. Journal of Virology 51:445–451
    [Google Scholar]
  9. Iorio R. M., Bratt M. A. 1984c; Monoclonal antibodies as functional probes of the HN glycoprotein of Newcastle disease virus: antigenic separation of the hemagglutinating and neuraminidase sites. Journal of Immunology 133:2215–2219
    [Google Scholar]
  10. Iorio R. M., Bratt M. A. 1985; Selection of unique antigenic variants of Newcastle disease virus with neutralizing monoclonal antibodies and anti-immunoglobulin. Proceedings of the National Academy of Sciences, U.S.A. 82:7106–7110
    [Google Scholar]
  11. Iorio R. M., Lawton K. A., Nicholson P. M., Bratt M. A. 1984; Monoclonal antibodies identify a strain-specific epitope on the HN glycoprotein of Newcastle disease virus. Virus Research 1:513–525
    [Google Scholar]
  12. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  15. Merz D. C., Scheid A., Choppin P. W. 1980; Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection. Journal of Experimental Medicine 151:275–288
    [Google Scholar]
  16. Nishikawa K., Isomura S., Suzuki S., Watanabe E., Hameguchi M., Yoshida T., Nagai Y. 1983; Monoclonal antibodies to the HN glycoprotein of Newcastle disease virus. Biological characterization and use for strain comparisons. Virology 130:318–330
    [Google Scholar]
  17. Peeples M. E., Bratt M. A. 1982; Virion functions of RNA+ temperature-sensitive mutants of Newcastle disease virus. Journal of Virology 41:965–973
    [Google Scholar]
  18. Peeples M. E., Gallagher I. P., Bratt M. A. 1981; Permissive temperature analysis of RNA+ temperature-sensitive mutants of Newcastle disease virus. In The Replication of Negative Strand Viruses pp 567–573 Edited by Bishop D. H. L., Compans R. W. New York: Elsevier;
    [Google Scholar]
  19. Peeples M. E., Glickman R. L., Bratt M. A. 1983; Thermostabilities of virion activities of Newcastle disease virus: evidence that the temperature-sensitive mutants in complementation groups B, BC and C have altered HN protein. Journal of Virology 45:18–26
    [Google Scholar]
  20. Pennington T. H. 1978; Antigenic differences between strains of Newcastle disease virus. Archives of Virology 56:345–351
    [Google Scholar]
  21. Russell P. H., Alexander D. J. 1983; Antigenic variation of Newcastle disease virus strains detected by monoclonal antibodies. Archives of Virology 75:245–253
    [Google Scholar]
  22. Scheid A., Choppin P. W. 1973; Isolation and purification of the envelope proteins of Newcastle disease virus. Journal of Virology 11:263–271
    [Google Scholar]
  23. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  24. Tsipis J. E., Bratt M. A. 1976; Isolation and preliminary characterization of temperature-sensitive mutants of Newcastle disease virus. Journal of Virology 18:848–855
    [Google Scholar]
  25. Weiss S. R., Bratt M. A. 1974; Polyadenylate sequences on Newcastle disease virus mRNA synthesized in vivo and in vitro . Journal of Virology 13:1220–1230
    [Google Scholar]
  26. Wilson M. B., Nakane P. K. 1978; Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In Immunofluorescence and Related Staining Techniques pp 215–224 Edited by Knapp W., Holubar K., Wich G. New York, Amsterdam & Oxford: Elsevier/North-Holland;
    [Google Scholar]
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