1887

Abstract

Summary

The 28000 mol. wt. polypeptide (p28) of adult T-cell leukaemia-associated antigen encoded by the 24S defective human T-cell leukaemia virus (HTLV-I) is associated with protein kinase activity. We have determined the nucleotide sequence of this defective HTLV-I provirus and found that it contains a portion of the gene (p19 and part of p24), the pX region, and two long terminal repeats, one at each end. The predicted p28 -pX fused protein consists of 190 amino acids and its mol. wt. was calculated as 21055. The results of peptide mapping analysis showing that p28 contains p19 supported the nucleotide sequence data. That p28 was encoded by this defective provirus was also demonstrated by transient expression of p28 polypeptide in COS 7 cells transfected with a recombinant plasmid containing a simian virus 40 early promoter and the p28-coding region of the 24S HTLV-I.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-7-1373
1986-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/7/JV0670071373.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-7-1373&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Stedman J. D. 1978; Efficient fluorography of 3H and 14C on thin layers. Analytical Biochemistry 89:247–256
    [Google Scholar]
  2. Catovsky D., Greaves M. F., Rose M., Galton D. A. G., Goolden A. W. G., Mcclusky D. R., White I. M., Lampert I., Bourikas G., Ireland R., Brownell A. I., Bridges J. M., Blattner W. A., Gallo R. C. 1982; Adult T-cell lymphoma-leukemia in blacks from the West Indies. Lancet i:639–643
    [Google Scholar]
  3. Chamberlain J. P. 1979; Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Analytical Biochemistry 98:132–135
    [Google Scholar]
  4. Elder J. H., Pickett R. A. II, Hampton J., Lerner R. A. 1977; Radioiodination of proteins in single polyacrylamide gel slices. Journal of Biological Chemistry 252:6510–6515
    [Google Scholar]
  5. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  6. Hattori S., Imagawa K., Shimizu F., Hashimura E., Seiki M., Yoshida M. 1983; Identification of envelope glycoprotein encoded by env gene of human T-cell leukemia virus. Gann 74:790–793
    [Google Scholar]
  7. Hinuma Y., Nagata K., Hanaoka M., Nakai M., Matsumoto T., Kinoshita K., Shirakawa S., Miyoshi I. 1981; Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proceedings of the National Academy of Sciences, U.S.A. 78:6476–6480
    [Google Scholar]
  8. Kalyanaraman V. S., Sarngadharan M. G., Poiesz B. J., Ruscetti F. W., Gallo R. C. 1981; Immunological properties of a type C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retroviruses. Journal of Virology 38:906–915
    [Google Scholar]
  9. Kalyanaraman V. S., Sarngadharan M. G., Nakano Y., Aoki T., Gallo R. C. 1982; Natural antibodies to the structural core protein (p24) of the human T-cell leukemia (lymphoma) retrovirus found in sera of leukemia patients in Japan. Proceedings of the National Academy of Sciences, U.S.A. 79:1653–1657
    [Google Scholar]
  10. Karess R. E., Hanafusa H. 1981; Viral and cellular src genes contribute to the structure of recovered avian sarcoma virus transforming protein. Cell 24:155–164
    [Google Scholar]
  11. Kobayashi N., Yamamoto N., Koyanagi Y., Schneider J., Hunsmann G., Hatanaka M. 1984a; Translation of HTLV (human T-cell leukemia virus) RNA in a nuclease-treated rabbit reticulocyte system. EMBO Journal 3:321–325
    [Google Scholar]
  12. Kobayashi N., Konishi H., Sabe H., Shigesada K., Noma T., Honjo T., Hatanaka M. 1984b; Genomic structure of HTLV (human T-cell leukemia virus): detection of defective genome and its amplification in MT-2 cells. EMBO Journal 3:1339–1343
    [Google Scholar]
  13. Kobayashi N., Koyanagi Y., Yamamoto N., Hinuma Y., Sato H., Okochi K., Hatanaka M. 1984c; 28, 000-dalton polypeptide of adult T-cell leukemia-associated antigen encoded by 24S mRNA of human T-cell leukemia virus has an associated protein kinase activity. Journal of Biological Chemistry 259:11162–11164
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Manzari V., Wong-Staal F., Franchini G., Colombini S., Gelman E. P., Oroszlan S., Staal S., Gallo R. C. 1983; Human T-cell leukemia-lymphoma virus (HTLV): cloning of an integrated defective provirus and flanking cellular sequences. Proceedings of the National Academy of Sciences, U.S.A. 80:1574–1578
    [Google Scholar]
  16. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  17. Miyoshi I., Kubonishi I., Yoshimoto S., Shiraishi Y. 1981; A T-cell line derived from normal human cord leukocytes by co-culturing with human leukemic T-cells. Gann 72:978–981
    [Google Scholar]
  18. O’farrell P. Z., Goodman H. M., O’farrell P. H. 1977; High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:1133–1142
    [Google Scholar]
  19. O’hare K., Benoist C., Breathnach R. 1981; Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase. Proceedings of the National Academy of Sciences, U.S.A. 78:1527–1531
    [Google Scholar]
  20. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. 1980; Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proceedings of the National Academy of Sciences, U.S.A. 77:7415–7419
    [Google Scholar]
  21. Robert-Guroff M., Ruscetti F. W., Posner L. E., Poiesz B. J., Gallo R. C. 1981; Detection of the human T cell lymphoma virus pi9 in cells of some patients with cutaneous T cell lymphoma and leukemia using a monoclonal antibody. Journal of Experimental Medicine 154:1957–1964
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  23. Sarin P. S., Aoki T., Shibata A., Ohnishi Y., Aoyagi Y., Miyakoshi H., Emura I., Kalyanaraman V. S., Robert-Guroff M., Popovic M., Nowell P. C., Gallo R. C. 1983; High incidence of human type-C retrovirus (HTLV) in family members of a HTLV-positive Japanese T-cell leukemia patient. Proceedings of the National Academy of Sciences, U.S.A. 80:2370–2374
    [Google Scholar]
  24. Seiki M., Hattori S., Hirayama Y., Yoshida M. 1983; Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proceedings of the National Academy of Sciences, U.S.A. 80:3618–3622
    [Google Scholar]
  25. Shimotohno K., Golde D. W., Miwa M., Sugimura T., Chen I. S. Y. 1984; Nucleotide sequence analysis of the long terminal repeat of human T-cell leukemia virus type II. Proceedings of the National Academy of Sciences, U.S.A. 81:1079–1083
    [Google Scholar]
  26. Takatsuki K., Uchiyama T., Sagawa K., Yodoi J. 1977; Adult T cell leukemia in Japan. In Topics in Hematology pp 73–77 Edited by Seno S., Takaku F., Irino S. Amsterdam: Excerpta Medica;
    [Google Scholar]
  27. Tanaka Y., Koyanagi Y., Chosa T., Yamamoto N., Hinuma Y. 1983; Monoclonal antibody reactive with both p28 and pl9 of adult T-cell leukemia virus-specific polypeptides. Gann 74:327–330
    [Google Scholar]
  28. Uchiyama T., Yodoi J., Sagawa K., Takatsuki K. 1977; Adult T-cell leukemia: clinical and hematologic features of 16 cases. Blood 50:481–492
    [Google Scholar]
  29. Van Beveren C., Van Straaten F., Galleshaw J. A., Verma I. M. 1981; Nucleotide sequence of the genome of a murine sarcoma virus. Cell 27:97–108
    [Google Scholar]
  30. Yamamoto N., Hinuma Y. 1982; Antigens in an adult T-cell leukemia virus-producer cell line: reactivity with human serum antibodies. International Journal of Cancer 30:289–293
    [Google Scholar]
  31. Yamamoto N., Schneider J., Hinuma Y., Hunsmann G. 1982a; Adult T-cell leukemia-associated antigen (ATLA): detection of a glycoprotein in cell-and virus-free supernatant. Zeitschrift für Naturforschung 37c:731–732
    [Google Scholar]
  32. Yamamoto N., Okada M., Koyanagi Y., Kannagi M., Hinuma Y. 1982b; Transformation of human leukocytes by cocultivation with an adult T-cell leukemia virus producer cell line. Science 217:737–739
    [Google Scholar]
  33. Yoshida M., Miyoshi I., Hinuma Y. 1982; Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease. Proceedings of the National Academy of Sciences, U.S.A. 79:2031–2035
    [Google Scholar]
  34. Zakut-Houri R., Oren M., Bienz B., Lavie V., Hazum S., Givol D. 1983; A single gene and a pseudogene for the cellular tumor antigen p53. Nature, London 306:594–597
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-7-1373
Loading
/content/journal/jgv/10.1099/0022-1317-67-7-1373
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error