1887

Abstract

Summary

The dominant neomycin resistance gene (neo) was introduced into the genome of the myeloproliferative sarcoma virus (MPSV), a replication-defective retrovirus carrying the oncogene. The resulting selectable neo-MPSV virus did not lose its acute transforming property, unlike the results of attempts by other groups to insert marker genes into oncogenic viruses. Neo-MPSV DNA was used to generate infectious virus by transfection followed by rescue with Friend or Moloney murine leukaemia virus. Infection of fibroblasts with this virus resulted in morphologically transformed cells which were resistant to the neomycin analogue G418. Segregation of the two functions (transformation and G418 resistance) was not observed in more than 500 independent viral transfers to fibroblasts. Furthermore, neo-MPSV retained the leukaemogenesis-inducing properties of the wild-type virus. Myeloproliferation and G418-resistance transfer did not segregate after passage in mice.

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1986-07-01
2021-10-24
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References

  1. Auffray C., Rougeon F. 1980; Purification of mouse immunoglobulin heavy-chain messenger RNA from total myeloma tumor RNA. European Journal of Biochemistry 107:303–314
    [Google Scholar]
  2. Bilello J. A., Colletta G., Warnecke G., Koch G., Frisby D., Pragnell I. B., Ostertag W. 1980; Analysis of the expression of SFFV related RNA and gp55, a Friend and Rauscher virus specific protein. Virology 107:331–344
    [Google Scholar]
  3. Blair D. G., Oskarsson M., Wood T. G., Mcclements W. L., Fischinger P. J., Vande Woude G. 1981; Activation of the transforming potential of a normal cell sequence -a molecular model for oncogenesis. Science 212:941–943
    [Google Scholar]
  4. Chang A. C. Y., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  5. Chen I. S. Y., Temin H. M. 1982; Substitution of 5′ helper virus sequences into non-rel portion of reticuloendotheliosis virus strain T suppresses transformation of chicken spleen cells. Cell 31:111–120
    [Google Scholar]
  6. Colbére-Garapin F., Horodniceanu F., Kourilsky P., Garapin A. 1981; A new dominant hybrid selective marker for higher eukaryotic cells. Journal of Molecular Biology 150:1–14
    [Google Scholar]
  7. Der C., Kroutins T., Cooper G. 1982; Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses. Proceedings of the National Academy of Sciences, U.S.A. 79:3637–3640
    [Google Scholar]
  8. Emerman M., Temin H. M. 1984; Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism. Cell 39:459–467
    [Google Scholar]
  9. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  10. Hwang L. -H. S., Gilboa E. 1984; Expression of genes introduced into cells by retroviral infection is more efficient than that of genes introduced into cells by DNA transfection. Journal of Virology 50:417–424
    [Google Scholar]
  11. Hwang L. -H. S., Park J., Gilboa E. 1984; Role of intron-contained sequences in formation of Moloney murine leukemia virus env mRNA. Molecular and Cellular Biology 4:2289–2297
    [Google Scholar]
  12. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. 1979; A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Molecular and General Genetics 177:65–72
    [Google Scholar]
  13. Joyner A., Bernstein A. 1983a; Retrovirus transduction: generation of infectious retroviruses expressing dominant and selectable genes is associated with in vivo recombination and deletion events. Molecular and Cellular Biology 3:2180–2190
    [Google Scholar]
  14. Joyner A., Bernstein A. 1983b; Retrovirus transduction: segregation of the viral transforming function and the herpes simplex virus gene in infectious Friend spleen focus-forming virus thymidine kinase vectors. Molecular and Cellular Biology 3:2191–2202
    [Google Scholar]
  15. Kollek R., Stocking C., Smadja-Joffe I., Ostertag W. 1984; Molecular cloning and characterization of a leukemia inducing myeloproliferative sarcoma virus and two of its temperature-sensitive mutants. Journal of Virology 50:717–724
    [Google Scholar]
  16. Lewis S., Gifford A., Baltimore D. 1984; Joining of VK to J, gene segments in a retroviral vector introduced into lymphoid cells. Nature, London 308:425–428
    [Google Scholar]
  17. Mcmaster G. K., Carmichael G. G. 1977; Analysis of single-and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A. 74:4835–4839
    [Google Scholar]
  18. Mann R., Mulligan R. C., Baltimore D. 1983; Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33:153–159
    [Google Scholar]
  19. Miller A. D., Ong E. S., Rosenfeld M. G., Verma I. M., Evans R. M. 1984; Infectious and selectable retrovirus containing an inducible rat growth hormone minigene. Science 225:993–998
    [Google Scholar]
  20. Narayanan R., Srinivasan A., Aaronson S. A. 1984; Sequences in the long terminal repeats of the Moloney murine sarcoma virus-124 genome which control transforming gene function. Virology 137:32–40
    [Google Scholar]
  21. Nash M., Brown N. V., Wong J. L., Arlinghaus R. B., Murphy E. C. Jr 1984; S1 nuclease mapping of viral RNAs from a temperature-sensitive transformation mutant of murine sarcoma virus. Journal of Virology 50:478–488
    [Google Scholar]
  22. Oskarsson M., Mcclements W. L., Blair D. G., Maizel J. V., Vande Woude G. F. 1980; Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus. Science 207:1222–1224
    [Google Scholar]
  23. Ostertag W., Pragnell I. B. 1982; Differentiation and viral involvement in differentiation of transformed mouse and erythroid cells. Current Topics in Microbiology and Immunology 94/95:143–208
    [Google Scholar]
  24. Ostertag W., Vehmeyer K., Fagg B., Pragnell I. B., Paetz W., Lebousse M. C., Smadja-Joffe F., Klein B., Jasmin C., Eisen H. 1980; Myeloproliferative virus, a cloned murine sarcoma virus with spleen focus-forming properties in adult mice. Journal of Virology 33:573–583
    [Google Scholar]
  25. Ostertag W., Freshney M., Vehmeyer K., Jasmin C., Rutter G. 1984; Action of temperature-sensitive mutants of myeloproliferative sarcoma virus suggests that fibroblast-transforming and hematopoietic transforming viral properties are related. Journal of Virology 49:173–181
    [Google Scholar]
  26. Parada L., Tabin C., Shuh C., Weinberg R. 1982; Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene. Nature, London 297:474–478
    [Google Scholar]
  27. Perkins A. S., Kirschmeier P. T., Gattoni-Celli S., Weinstein I. B. 1983; Design of a retrovirus-derived vector for expression and transduction of exogenous genes in mammalian cells. Molecular and Cellular Biology 3:1123–1132
    [Google Scholar]
  28. Pragnell I. B., Ostertag W., Paul J. 1977; The expression of viral and globin genes during differentiation of the Friend cell. Experimental Cell Research 108:269–278
    [Google Scholar]
  29. Pragnell I. B., Fusco A., Arbuthnott C., Smadja-Joffe F., Klein B., Jasmin C., Ostertag W. 1981; Analysis of the myeloproliferation sarcoma virus genome: limited changes in the prototype lead to altered target cell specificity. Journal of Virology 38:952–957
    [Google Scholar]
  30. Santos E., Tronick S., Aaronson S., Pulciani S., Barbacid M. 1982; T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of Balb-and Harvey-MSV transforming genes. Nature, London 298:343–347
    [Google Scholar]
  31. Shimotohno K., Temin H.M. 1981; Formation of infectious progeny virus after insertion of herpes simplex thymidine kinase gene into DNA of an avian retrovirus. Cell 26:67–77
    [Google Scholar]
  32. Southern E. M. 1975; Dectection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–513
    [Google Scholar]
  33. Stacey A., Arbuthnott C., Kollek R., Coggins L., Ostertag W. 1984; Comparison of myeloproliferative sarcoma virus with Moloney murine sarcoma virus variants by nucleotide sequencing and heteroduplex analysis. Journal of Virology 50:725–732
    [Google Scholar]
  34. Stocking C., Kollek R., Bergholz U., Ostertag W. 1985; Long terminal repeat sequences impart hematopoietic transformation property to the myeloproliferative sarcoma virus. Proceedings of the National Academy of Sciences, U.S.A. 82:5746–5750
    [Google Scholar]
  35. Tabin C. J., Hoffmann J. W., Goff S. P., Weinberg R. A. 1982; Adaptation of a retrovirus as a eukaryotic vector transmitting the herpes simplex virus thymidine gene. Molecular and Cellular Biology 2:426–436
    [Google Scholar]
  36. Tarpley W. G., Temin H. M. 1984; The location of v-src in a retrovirus vector determines whether the virus is toxic or transforming. Molecular and Cellular Biology 4:2653–2660
    [Google Scholar]
  37. Topp W. C. 1981; Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113:408–411
    [Google Scholar]
  38. Varmus H. E., Quintrell N., Ortiz S. 1981; Retroviruses as mutagens: insertion and excision of a nontransforming provirus alter expression of a resident transforming provirus. Cell 25:23–36
    [Google Scholar]
  39. Wei C. -M., Gibson M., Spear P. G., Scolnick E. M. 1981; Construction and isolation of a transmissible retrovirus containing the scr gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1. Journal of Virology 39:935–944
    [Google Scholar]
  40. Weiss R., Teich N. M., Varmus H., Coffin J. (editors) 1982 RNA Tumor Viruses, (Molecular Biology of Tumor Viruses, 2nd edn.) New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  41. Wigler M., Silverstein S., Lih-Syng L., Pellicer A., Yung-Chi C., Axel R. 1977; Transfer of purified herpes simplex virus thymidine kinase gene to cultured mouse cells. Cell 11:223–232
    [Google Scholar]
  42. Wigler M., Pellicer A., Silverstein S., Axel R. 1978; Biochemical transfer of single-copy eukaryotic genes using total cellular DNA as donor. Cell 14:725–731
    [Google Scholar]
  43. Williams D. A., Lemischka I. R., Nathan D. G., Mulligan R. C. 1984; Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse. Nature, Ixmdon 310:476–480
    [Google Scholar]
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