1887

Abstract

Summary

Polypeptides synthesized in Vero cells infected with African swine fever virus (ASFV) can be divided into early and late classes on the basis of their sensitivity to cytosine arabinoside. As ASFV does not inhibit cell protein synthesis until late in infection, immunoprecipitation was used to identify virus-specific polypeptides. Eighteen early and 15 late polypeptides were detected by polyacrylamide gel electrophoresis. Early polypeptides can be further divided into those which are transiently expressed at early times and the majority which are synthesized throughout infection. translation products of RNAs from infected cells at different stages of infection were compared with products after immunoprecipitation. A good correspondence was observed between the and patterns. Alle arly RNAs translated were synthesized in the presence of cytosine arabinoside and cycloheximide and can therefore be classified as immediate early RNAs. Only two polypeptides transcribed from early RNA were not present among late RNA products. No evidence was obtained for a discrete class of delayed early RNAs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-7-1343
1986-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/7/JV0670071343.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-7-1343&mimeType=html&fmt=ahah

References

  1. Baczko K., Carter M. J., Billeter M., Ter Meulen V. 1984; Measles virus gene expression in subacute sclerosing panencephalitis. Virus Research 1:585–595
    [Google Scholar]
  2. Blatter D. P., Garner F., Van Slyke K., Bradley A. 1972; Quantitative electrophoresis in polyacrylamide gels of 2–40%. Journal of Chromatography 64:147–155
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Brown G. D., Moyer R. W. 1983; The white pock mutants of rabbit poxvirus. V. In vitro translation of early host range mutant RNA. Virology 126:381–390
    [Google Scholar]
  5. Enjuanes L., Carrascosa A. L., Viñuela E. 1976; Isolation and properties of the DNA of African swine fever (ASF) virus. Journal of General Virology 32:479–492
    [Google Scholar]
  6. Kuznar J., Salas M. L., Viñuela E. 1980; DNA-dependent RNA polymerase in African swine fever virus. Virology 101:169–175
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Letchworth G. J., Whyard T. C. 1984; Characterization of African swine fever virus antigenic proteins by immunoprecipitation. Archives of Virology 80:265–274
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:56–58
    [Google Scholar]
  11. Ortin J., Enjuanes L., Viñuela E. 1979; Crosslinks in African swine fever virus DNA. Journal of Virology 31:579–583
    [Google Scholar]
  12. Salas M. L., Kuznar J., Viñuela E. 1981; Polyadenylation, methylation and capping of the RNA synthesized in vitro by African swine fever virus. Virology 113:489–491
    [Google Scholar]
  13. Sogo J. M., Almendral J. M., Talavera A., Viñuela E. 1984; Terminal and internal inverted repetitions in African swine fever virus DNA. Virology 133:271–275
    [Google Scholar]
  14. Tabarés E., Marcotegui M., Fernandez M., Sanchez Botija C. 1980a; Proteins specified by African swine fever virus. I. Analysis of viral structural proteins and antigenic properties. Archives of Virology 66:107–117
    [Google Scholar]
  15. Tabarés E., Martinez J., Ruiz Gonzalvo F., Sanchez Botija C. 1980b; Proteins specified by African swine fever virus. 11. Analysis of proteins in infected cells and antigenic properties. Archives of Virology 66:119–132
    [Google Scholar]
  16. Vigário J. D., Relvas M. E., Ferraz F. P., Ribeiro J. M., Pereira C. G. 1967; Identification and localization of genetic material of African swine fever virus by autoradiography. Virology 33:173–175
    [Google Scholar]
  17. Viñuela E. 1985; African swine fever virus. In Iridoviridae pp 152–170 Edited by Willis D. B. Berlin: Springer-Verlag;
    [Google Scholar]
  18. Wardley R. C., Andrade C. DE M., Black D. N., Castro Portugal F. L., Enjuanes L., Hess W. R., Mebus C., Ordas A., Rutili D., Sanchez Vizcaino J., Vigário J. D., Wilkinson P. J., Moura Nunes J. F., Thomson G. 1983; African swine fever virus. Archives of Virology 76:73–90
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-7-1343
Loading
/content/journal/jgv/10.1099/0022-1317-67-7-1343
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error