1887

Abstract

Summary

Filamentous particles of rice stripe virus (RSV) were found to be associated with an RNA-dependent RNA polymerase activity. The enzyme catalysed the synthesis of single- and double-stranded RNAs corresponding in size with RNA found in RSV particles, as well as two other dsRNAs with mol. wt. of 1.0 × 10 and 0.56 × 10. The RSV polymerase activity required Mg, Mn or Fe but not Na, K or NH which were inhibitory at concentrations of more than 50 m. The optimum pH of the polymerase was around 8·0 at the optimum temperature, 40°C. Detergent treatment of RSV particles did not enhance the polymerase activity. Purified RSV particles contained a minor polypeptide (mol. wt. 230000) as well as coat protein; the former may be the RSV polymerase. The taxonomy of RSV is discussed in relation to enveloped animal viruses with negative-stranded RNA, which also contain an RNA-dependent RNA polymerase in virus particles.

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/content/journal/jgv/10.1099/0022-1317-67-7-1247
1986-07-01
2020-11-25
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-7-1247
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