1887

Abstract

Summary

A wild-type isolate (WT) of soil-borne wheat mosaic virus (SBWMV, isolate JT) (RNA I 6.9 kb and RNA II 3.6 kb) was successively transferred to wheat plants by mechanical inoculation and a deletion mutant (DM) (RNA I 6.9 kb and RNA II 2.1 kb) was produced. In wheat germ extracts, RNA I of both WT and DM directed synthesis of polypeptides having mol. wt. of 220000 (220K) and 150K, and RNA II of both WT and DM directed synthesis of 25K and 19K polypeptides. Incubation in wheat germ extracts of WT or DM virions purified with an alkaline buffer also gave 220K, 25K and 19K polypeptides as major products and a 150K polypeptide as a minor product. In rabbit reticulocyte lysates, RNA I of both WT and DM directed synthesis of only the 220K polypeptide, whereas WT RNA II produced 100K, 46K, 25K and 19K polypeptides and DM RNA II, 31K, 25K and 19K polypeptides. SBWMV DM antiserum precipitated polypeptides of 100K, 31K, 25K and 19K but not 220K and 46K. The 19K polypeptide was identified as the capsid protein from its electrophoretic mobility and its reaction with antiserum to virus particles. Thus, the differences between the translation products of WT and DM SBWMV were confined only to those coded by RNA II.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-7-1237
1986-07-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/7/JV0670071237.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-7-1237&mimeType=html&fmt=ahah

References

  1. Beier H., Barciszewska M., Krupp G., Mitnacht R., Gross H. I. 1984a; UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAsTyr with suppressor activity from tobacco plants. EMBO Journal 3:351–356
    [Google Scholar]
  2. Beier H., Barciszewska M., Sickinger H. -D. 1984b; The molecular basis for the differential translation of TMV RNA in tobacco protoplasts and wheat germ extracts. EMBO Journal 3:1091–1096
    [Google Scholar]
  3. Brakke M. K. 1971; Soil-borne wheat mosaic virus. Commonwealth Mycological Institute I Association of Applied Biologists Descriptions of Plant Viruses no. 77
    [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, U.S.A. 75:4801–4805
    [Google Scholar]
  5. Brosius J., Dull T. J., Noller H. F. 1980; Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, U.S.A. 77:201–204
    [Google Scholar]
  6. Chamberlain J. P. 1979; Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Analytical Biochemistry 98:132–135
    [Google Scholar]
  7. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  8. Dahlberg A. E., Dingman C. W., Peacock A. C. 1969; Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels. Journal of Molecular Biology 41:139–147
    [Google Scholar]
  9. Davies J. W., Aalbers A. M. J., Stuik E. J., Van Kammen A. 1977; Translation of cowpea mosaic virus RNA in a cell-free extract from wheat germ. FEBS Letters 77:265–269
    [Google Scholar]
  10. Dorner A. J., Semler B. L., Jackson R. J., Hanecak R., Duprey E., Wimmer E. 1984; In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate. Journal of Virology 50:507–514
    [Google Scholar]
  11. Dougherty W. G., Hiebert E. 1980; Translation of potyvirus RNA in a rabbit reticulocyte lysate: cell-free translation strategy and a genetic map of the potyviral genome. Virology 104:183–194
    [Google Scholar]
  12. Franssen H., Goldbach R., Broekhuijsen M., Moerman M., Van Kammen A. 1982; Expression of middle-component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells. Journal of Virology 41:8–17
    [Google Scholar]
  13. Goelet P., Lomonossoff G. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U.S.A. 79:5818–5822
    [Google Scholar]
  14. Gustafson G. D., Larkins B. A., Jackson A. O. 1981; Comparative analysis of polypeptides synthesized in vivo and in vitro by two strains of barley stripe mosaic virus. Virology 111:579–587
    [Google Scholar]
  15. Hsu Y. H., Brakke M. K. 1985; Cell-free translation of soil-borne wheat mosaic virus RNAs. Virology 143:272–279
    [Google Scholar]
  16. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  17. Kozak M. 1983; Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  19. Leary J. J., Brigati D. J., Ward D. C. 1983; Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNAor RNA immobilized on nitro-cellulose: bio-blots. Proceedings of the National Academy of Sciences, U.S.A. 80:4045–4049
    [Google Scholar]
  20. McMaster G. K., Carmichael G. C. 1977; Analysis of single- and double-stranded nucleic acids in polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A. 74:4835–4838
    [Google Scholar]
  21. Neeleman L., Lindhout P., Van Vloten-Doting L. 1985; Artifacts are responsible for the translational activity of polyribosome preparations isolated from alfalfa mosaic virus-infected tobacco leaves. Virology 147:422–430
    [Google Scholar]
  22. Paterson R., Knight C. A. 1975; Protein synthesis in tobacco protoplasts infected with tobacco mosaic virus. Virology 64:10–22
    [Google Scholar]
  23. Pelham H. R. B. 1978; Leaky UAG termination codon in tobacco mosaic virus RNA. Nature, London 272:469–471
    [Google Scholar]
  24. Rao A. S., Brakke M. K. 1970; Dark treatment of wheat inoculated with soil-borne wheat mosaic and barley stripe mosaic viruses. Phytopathology 60:714–716
    [Google Scholar]
  25. Shirako Y., Brakke M. K. 1984a; Two purified RNAs of soil-borne wheat mosaic virus are needed for infection. Journal of General Virology 65:119–127
    [Google Scholar]
  26. Shirako Y., Brakke M. K. 1984b; Spontaneous deletion mutation of soil-borne wheat mosaic virus RNA II. Journal of General Virology 65:855–858
    [Google Scholar]
  27. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  28. Wilson T. M. A. 1984; Cotranslational disassembly increases the efficiency of expression of TMV RNA in wheat germ cell-free extracts. Virology 138:353–356
    [Google Scholar]
  29. Ziegler V., Richards K., Guilley H., Jonard G., Putz C. 1985; Cell-free translation of beet necrotic yellow vein virus: readthrough of the coat protein cistron. Journal of General Virology 66:2079–2087
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-7-1237
Loading
/content/journal/jgv/10.1099/0022-1317-67-7-1237
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error