1887

Abstract

Summary

Nine isolates of pleural effusion disease agent or virus (PEDV) from treponema-infected rabbits in various countries were examined for pathogenicity and persistence in rabbits. The isolates showed a wide range of pathogenicity and were categorized into three groups according to the severity of the acute infection. Group 1 comprised isolates causing more than 50% mortality, group 2 isolates causing mortality below 50%, while group 3 comprised isolates causing almost subclinical infections. The range between group 1 and group 3 was similar to that observed with virulent and avirulent progeny of the original PEDV isolate. Infection by each of the nine isolates resulted in a chronic low level viraemia which persisted for up to 2 years or more. Viral progeny of pathogenic isolates obtained in serum after the 2nd month of infection failed to induce clinical disease on rabbit inoculation. The chronic, subclinical infection was associated with a moderate, continued increase in serum IgG, but circulating immune complexes could not be demonstrated. Two years after infection slight histopathological changes were present in lymph nodes, spleen, liver, heart and lung. Evidence of immune complex disease could not be demonstrated.

Keyword(s): PEDV , rabbit and virulence
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-6-993
1986-06-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/6/JV0670060993.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-6-993&mimeType=html&fmt=ahah

References

  1. Brandslund I., Siersted H. C., Jensenius J. C., Svehag Sv-E. 1981; Detection and quantitation of immune complexes with a rapid polyethylene glycol precipitation complement consumption method (PEG-CC). Methods in Enzymology 74:551–571
    [Google Scholar]
  2. Cafruny W. A., Plagemann P. G. W. 1982; Immune response to lactate dehydrogenase-elevating virus: isolation of infectious virus-immunoglobulin G complexes and quantitation of specific antiviral immunoglobulin G response in wild-type and nude mice. Infection and Immunity 37:1001–1006
    [Google Scholar]
  3. Christensen N., Fennestad K. L., Bruun L. 1978; Pleural effusion disease in rabbits. Histopathological observations. Acta pathologica et microbiologica scandinavica, Section A 86:251–256
    [Google Scholar]
  4. Fennestad K. L. 1985; Pathogenetic observations on pleural effusion disease in rabbits. Archives of Virology 84:163–174
    [Google Scholar]
  5. Fennestad K. L., Macnaughton M. R. 1983; Pleural effusion disease in rabbits. Properties of the aetiological agent. Archives of Virology 76:179–188
    [Google Scholar]
  6. Fennestad K. L., Skovgaard Jensen H-J., M⌀ller S., Weis Bentzon M. 1975; Pleural effusion disease in rabbits. Clinical and post mortem observations. Acta pathologica et microbiologica scandinavica, Section B 83:541–548
    [Google Scholar]
  7. Fennestad K. L., Bruun L., Wed⌀ E. 1980; Pleural effusion disease agent as passenger of Treponema pallidum suspensions from rabbits. Survey of laboratories. British Journal of Venereal Diseases 56:198–203
    [Google Scholar]
  8. Fennestad K. L., Mansa B., Larsen S. 1981; Pleural effusion disease in rabbits. Observations on viraemia, immunity and transmissibility. Archives of Virology 70:11–19
    [Google Scholar]
  9. Fennestad K. L., Bruun L., Haahr S. 1982; Interferon in rabbit sera after inoculation with Treponema pallidum suspensions contaminated with PED virus. British Journal of Venereal Diseases 58:143–146
    [Google Scholar]
  10. Gudjònsson H., Newman B., Turner T. B. 1970; Demonstration of a virus-like agent contaminating material containing the Stockholm substrain of the Nichols pathogenic. Treponema pallidum. British Journal of Venereal Diseases 46:435–440
    [Google Scholar]
  11. Gudjònsson H., Newman B., Turner T. B. 1972; Screening out a virus-like agent from the testicular suspension of the Nichols pathogenic T. pallidum. With observations on certain characteristics of the agent. British Journal of Venereal Diseases 48:102–107
    [Google Scholar]
  12. Larsen S. 1978; Immunofluorescent microscopy findings in minimal or no change disease and slight generalized mesangioproliferative glomerulonephritis. Acta pathologica et microbiologica scandinavica, Section A 86:531–542
    [Google Scholar]
  13. Mancini G., Carbonara A. O., Heremans J. F. 1965; Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2:235–254
    [Google Scholar]
  14. Menke H. E., Van Der Heijden C. A., Kruizinga W., Kruijt B. C., Osterhaus A. D. M. E. 1980; Intercurrent death of rabbits after inoculation with Treponema pallidum in the Netherlands. 7th ICLAS Symposium (Utrecht, 1979) pp 263–268 Stuttgart: Gustav Fischer-Verlag;
    [Google Scholar]
  15. Oldstone M. B. A., Dixon F. J. 1975; Immune complex disease associated with viral infections. In Viral Immunology and Immunopathology pp 341–356 Edited by Notkins A. L. New York: Academic Press;
    [Google Scholar]
  16. Porter D. D., Porter H. G., Suffin S. C., Larsen A. E. 1984; Immunoglobulin classes of Aleutian disease virus antibody. Infection and Immunity 43:463–466
    [Google Scholar]
  17. Riley V., Lilly F., Huerto E., Bardell D. 1960; Transmissible agent associated with 26 types of experimental mouse neoplasms. Science 132:545–547
    [Google Scholar]
  18. Rowson K. E. K., Mahy B. W. J. 1975; Lactic dehydrogenase virus. Virology Monographs vol 13 pp 1–121 Vienna & New York: Springer-Verlag;
    [Google Scholar]
  19. Small J. D., Aurelian L., Squire R. A., Strandberg J. D., Melby E. C., Turner T. B., Newman B. 1979; Rabbit cardiomyopathy associated with a virus antigenically related to human coronavirus strain 229 E. American Journal of Pathology 95:709–728
    [Google Scholar]
  20. Vaisman A., Paris-Hamelin A., Catalan F., Dunoyer F. 1973; Entretien au laboratoire des souches de tréponèmes pathogènes. Precautions a prendre. Prophylaxie sanitaire et morale 45:71–73
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-6-993
Loading
/content/journal/jgv/10.1099/0022-1317-67-6-993
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error