1887

Abstract

Summary

Multiply cloned variants of vesicular stomatitis virus (VSV) were found to generate/amplify defective interfering (DI) particles at a rate greatly exceeding the rates normally observed for wild-type VSV (or for other mutants of VSV). A single undiluted passage of the first clonal pool of this variant virus produced concentrated visible bands of DI particles on sucrose gradients whereas wild-type and other mutant strains of VSV required from three to six or more serial undiluted passages. Since DI particle amplication by wild-type VSV at each undiluted passage can exceed 10000-fold enrichment, these variant virus clones were generating/amplifying DI particles many millions of times more rapidly than were wild-type and other mutant strains of VSV. This rate of generation/amplification is so high that it was not feasible to obtain accurate estimates of the rates of generation (or amplification) of these DI particles.

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/content/journal/jgv/10.1099/0022-1317-67-6-1195
1986-06-01
2022-01-19
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References

  1. Barrett A. D. T., Crouch C. F., Dimmock N. J. 1981; Assay of defective-interfering Semliki Forest virus- specified RNAs. Journal of General Virology 54:273–280
    [Google Scholar]
  2. Cole C. N., Baltimore D. 1973; Defective interfering particles of poliovirus. IV. Mechanisms of enrichment. Journal of Virology 12:1414–1426
    [Google Scholar]
  3. Crick J., Brown F. 1974; An interfering component of rabies virus which contains RNAs. Journal of General Virology 22:147–151
    [Google Scholar]
  4. Holland J. J., Villarreal L. P. 1974; Persistent noncytocidal vesicular stomatitis virus infections mediated by defective T particles that suppress virion transcriptase. Proceedings of the National Academy of Sciences, U.S.A 71:2956–2960
    [Google Scholar]
  5. Holland J. J., Villarreal L. P. 1975; Purification of defective interfering T particles of vesicular stomatitis and rabies viruses generated in vivo in brains of newborn mice. Virology 67:438–449
    [Google Scholar]
  6. Holland J. J., Villarreal L. P., Breindl M. 1976a; Factors involved in the generation and replication of rhabdovirus defective interfering T particles. Journal of Virology 17:805–815
    [Google Scholar]
  7. Holland J. J., Villarreal L. P., Breindl M., Semler B. L., Kohne D. 1976b; Defective interfering virus particles attenaute virus lethality in vivo and in vitro. . In Animal Virology pp 773–786 Edited by Baltimore D., Huang A. S., Fox C. F. New York: Academic Press;
    [Google Scholar]
  8. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., Vandepol S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  9. Horodyski F. M., Nichol S. T., Spindler K. R., Holland J. J. 1983; Properties of DI particle resistant mutants of vesicular stomatitis virus isolated from persistent infections and from undiluted passages. Cell 33:801–810
    [Google Scholar]
  10. Huang A. S. 1977; Viral pathogenesis and molecular biology. Bacteriological Reviews 41:811–821
    [Google Scholar]
  11. Janda J. M., Davis A. R., Nayak D. P., De B. K. 1979; Diversity and generation of defective interfering influenza virus particles. Virology 95:48–58
    [Google Scholar]
  12. Kawai A., Matsumoto S. 1982; A sensitive bioassay for detecting defective interfering particles of rabies virus. Virology 122:98–108
    [Google Scholar]
  13. Kolakofsky D. 1979; Studies on the generation and amplification of Sendai virus defective-interfering genomes. Virology 93:589–593
    [Google Scholar]
  14. Kolakofsky D. 1982; Isolation of vesicular stomatitis virus defective interfering genomes with different amounts of 5' complementarity. Journal of Virology 41:566–574
    [Google Scholar]
  15. Leppert M., Kort L., Kolakofsky D. 1977; Further characterization of Sendai virus DI RNA’s: a model for their generation. Cell 12:539–552
    [Google Scholar]
  16. McClure M. A., Holland J. J., Perrault J. 1980; Generation of defective interfering particles in picornaviruses. Virology 100:408–418
    [Google Scholar]
  17. Norkin L. C., Tirrell S. M. 1982; Emergence of simian virus 40 variants during serial passage of plaque isolates. Journal of Virology 42:730–733
    [Google Scholar]
  18. Popescu M., Schaefer H., Lehmann-Grube F. 1976; Homologous interference of lymphocytic choriomeningitis virus: detection and measurement of interference foci-forming units. Journal of Virology 20:1–8
    [Google Scholar]
  19. Pringle C. R. 1970; Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine and ethylmethane sulfonate. Journal of Virology 5:559–567
    [Google Scholar]
  20. Pringle C. R. 1977; Genetics of rhabdoviruses. In Comprehensive Virology vol 9 pp 239–289 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  21. Radloff R. J., Young S. A. 1983; Defective interfering particles of encephalomyocarditis virus. Journal of General Virology 64:1637–1641
    [Google Scholar]
  22. Rowlands D., Grabau E., Spindler K., Jones C., Semler B., Holland J. J. 1980; Virus protein changes and RNA terminal alterations evolving during persistent infection. Cell 19:871–880
    [Google Scholar]
  23. Spindler K. R., Horodyski F. M., Holland J. J. 1982; High multiplicities of infection favor rapid and random evolution of vesicular stomatitis virus. Virology 119:96–108
    [Google Scholar]
  24. Stampfer M., Baltimore D., Huang A. S. 1971; Absence of interference during high multiplicity infection by clonally purified vesicular stomatitis virus. Journal of Virology 7:409–411
    [Google Scholar]
  25. Szilagyi J. F., Pringle C. R. 1979; Effect of temperature-sensitive mutation on activity of the RNA transcriptase of vesicular stomatitis virus New Jersey. Journal of Virology 30692–700
    [Google Scholar]
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