1887

Abstract

Summary

Semliki Forest virus (SFV) normally causes an acute lethal encephalitis in mice following intranasal inoculation. However, animals co-administered with 10 LD SFV and defective interfering (DI) SFV survive the infection without clinical signs of disease. In this report we demonstrate the isolation of infectious virus from the brains of 12/169 protected mice up to 6.5 months post-infection. Although, with one exception, mice were clinically normal, five of 12 of the SFV isolates were identical to the original virus as judged by plaque morphology, maximum temperature for growth, virulence in mice and pathology. Others were less virulent (although not any were plaque or temperature-sensitive mutants) and on re-inoculation into fresh mice caused a demyelinating pathology which was not an attribute of the original inoculum. How the virulent virus can persist in brain, sometimes in amounts in excess of 100 LD, without causing disease remains to be determined.

Keyword(s): DI virus , persistent infection and SFV
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1986-06-01
2024-03-28
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References

  1. Ahmed R., Salmi A., Butler L. D., Chiller J. M., Oldstone M. B. A. 1984; Selection of genetic variants of lymphocytic choriomeningitis virus in spleens of persistently infected mice. Journal of Experimental Medicine 160:521–540
    [Google Scholar]
  2. Barrett A. D. T., Dimmock N. J. 1984a; Properties of host and virus which influence defective interfering virus- ediated protection of mice against Semliki Forest virus lethal encephalitis. Archives of Virology 81:185–188
    [Google Scholar]
  3. Barrett A. D. T., Dimmock N. J. 1984b; Modulation of Semliki Forest virus-induced infection of mice by defective interfering virus. Journal of Infectious Diseases 150:98–104
    [Google Scholar]
  4. Barrett A. D. T., Dimmock N. J. 1984c; Modulation of a systemic Semliki Forest virus infection in mice by defective interfering virus. Journal of General Virology 65:1827–1831
    [Google Scholar]
  5. Barrett A. D. T., Dimmock N. J. 1986; Defective interfering viruses and infections of animals. Current Topics in Microbiology and Immunology 127: (in press)
    [Google Scholar]
  6. Barrett A. D. T., Crouch C. F., Dimmock N. J. 1981; Assay of defective-interfering Semliki Forest virus by the inhibition of synthesis of virus-specified RNAs. Journal of General Virology 54:273–280
    [Google Scholar]
  7. Barrett A. D. T., Crouch C. F., Dimmock N. J. 1984a; Defective interfering Semliki Forest virus populations are biologically and physically heterogeneous. Journal of General Virology 65:1273–1283
    [Google Scholar]
  8. Barrett A. D. T., Guest A. R., Mackenzie A., Dimmock N. J. 1984b; Protection of mice infected with a lethal dose of Semliki Forest virus by defective interfering virus: modulation of virus multiplication. Journal of General Virology 65:1909–1920
    [Google Scholar]
  9. Bradish C. J., Allner K., Maber H. B. 1971; The virulence of original and derived strains of Semliki Forest virus for mice, guinea-pigs and rabbits. Journal of General Virology 12:141–160
    [Google Scholar]
  10. Bradish C. J., Fitzgeorge R., Titmuss D., Baskerville A. 1979; The responses of nude-athymic mice to nominally avirulent togavirus infections. Journal of General Virology 42:555–566
    [Google Scholar]
  11. Casali P., Rice G. P. A., Oldstone M. B. A. 1984; Viruses disrupt functions of human lymphocytes. Journal of Experimental Medicine 159:1322–1337
    [Google Scholar]
  12. Crouch C. F., Mackenzie A., Dimmock N. J. 1982; The effect of defective interfering Semliki Forest virus on the histopathology of infection with virulent Semliki Forest virus in mice. Journal of Infectious Diseases 146:411–416
    [Google Scholar]
  13. Dimmock N. J., Kennedy S. I. T. 1978; Prevention of death in Semliki Forest virus-induced mice by administration of defective-interfering Semliki Forest virus. Journal of General Virology 39:231–242
    [Google Scholar]
  14. Doyle M., Holland J. J. 1973; Prophylaxis and immunisation in mice by use of virus-free defective T particles to protect against intra-cerebral infection by vesicular stomatitis virus. Proceedings of the National Academy of Sciences, U.S.A 70:2105–2108
    [Google Scholar]
  15. Fultz P. N., Shadduck J. A., Kang C.-Y., Streilein J. W. 1982a; Mediators of protection against lethal systemic vesicular stomatitis virus infection in hamsters: defective interfering particles, polyinosinate- polycytidylate, and interferon. Infection and Immunity 37:679–686
    [Google Scholar]
  16. Fultz P. N., Shadduck J. A., Kang C.-Y., Streilein J. W. 1982b; Vesicular stomatitis virus can establish persistent infections in Syrian hamsters. Journal of General Virology 63:493–497
    [Google Scholar]
  17. Fultz P. N., Holland J. I., Knobler R., Oldstone M. B. A. 1984; Long-term persistence by vesicular stomatitis virus in hamsters. In Nonsegmented Negative Strand Viruses pp 489–496 Edited by Bishop D. H. L., Compans R. W. New York: Academic Press;
    [Google Scholar]
  18. Holland J. J., Kennedy S. I. T., Semler B. L., Jones C. L., Roux L., Grabau E. A. 1980; Defective interfering RNA viruses and the host cell response. In Comprehensive Virology vol 16 pp 137–192 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  19. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  20. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. In Comprehensive Virology vol 10 pp 73–146 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  21. Jagelman S., Suckling A. J., Webb H. E., Bowen E. T. W. 1978; The pathogenesis of avirulent Semliki Forest virus infections in athymic nude mice. Journal of General Virology 41:599–607
    [Google Scholar]
  22. Jones C. L., Holland J. J. 1980; Requirements for DI particle prophylaxis against vesicular stomatitis virus infection in vivo.. Journal of General Virology 49:215–220
    [Google Scholar]
  23. Mims C. A. 1956; Rift Valley fever virus in mice. IV. Incomplete virus: its production and properties. British Journal of Experimental Pathology 31:129–143
    [Google Scholar]
  24. Morser M. J., Kennedy S. I. T., Burke D. C. 1973; Virus-specified polypeptides in cells infected with Semliki Forest virus. Journal of General Virology 21:19–29
    [Google Scholar]
  25. Perrault J. 1981; Origins and replication of defective interfering particles. Current Topics in Microbiology and Immunology 93:151–207
    [Google Scholar]
  26. Popescu M., Lehmann-Grube F. 1977; Defective interfering particles in mice infected with lymphocytic choriomeningitis virus. Virology 77:78–83
    [Google Scholar]
  27. Rabinowitz S. G., Huprikar J. 1979; The influence of defective interfering particles of the PR-8 strain of influenza A virus on the pathogenesis of pulmonary infection of mice. Journal of Infectious Diseases 140:305–315
    [Google Scholar]
  28. Rabinowitz S. G., Dal Canto M. C., Johnson T. C. 1977; Infection of the central nervous system produced by mixtures of defective-interfering particles and wildtype vesicular stomatitis virus in mice. Journal of Infectious Diseases 136:59–74
    [Google Scholar]
  29. Seamer J. H., Boulter E. A., Zlotnik I. 1971; Delayed onset of encephalitis in mice passively immunized against Semliki Forest virus. British Journal of Experimental Pathology 52:408–414
    [Google Scholar]
  30. Spandidos D. A., Graham A. F. 1976; Generation of defective virus after infection of newborn rats with reovirus. Journal of Virology 20:234–247
    [Google Scholar]
  31. Stark C., Kennedy S. I. T. 1978; The generation and propagation of defective-interfering particles of Semliki Forest virus in different cell types. Journal of General Virology 89:285–299
    [Google Scholar]
  32. Tan K. B., Sambrook J. F., Bellett A. J. D. 1969; Semliki Forest virus temperature-sensitive mutants: isolation and characterisation. Virology 38:427–439
    [Google Scholar]
  33. Von Magnus P. 1951; Propagation of the PR8 strain of influenza A virus in chick embryos. III. Properties of the incomplete virus produced in several passages of undiluted virus. Acta pathologica et microbiologica scandinavica 29:157–181
    [Google Scholar]
  34. Welsh R. M., Lampert P. W., Oldstone M. B. A. 1977; Prevention of virus-induced cerebellar disease by defective interfering lymphocytic choriomeningitis virus. Journal of Infectious Diseases 136:391–399
    [Google Scholar]
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