A competitive enzyme immunoassay was developed to detect antibodies to a glycoprotein (gI) of Aujeszky's disease virus. Infected cell monolayers were used as antigen and a monoclonal antibody directed against an epitope of gI as indicator antibody. It was demonstrated that pigs vaccinated with the Bartha, BUK or NIA-4 strains did not produce antibody to the epitope of gI, whereas all wild-type viruses tested did induce this antibody. The antibody to the gI epitope persisted for at least 15 weeks. The present test, which enables us to distinguish pigs vaccinated with certain attenuated strains from pigs infected with wild-type Aujeszky's disease virus, may be of great value in future combined vaccination-eradication programmes for Aujeszky's disease.
BernsA. J. M.,
QuintW.,
GielkensA. L. J.,
Van OirschotJ. T.1985; Construction of deletion mutants of pseudorabies virus: a new generation of live vaccines.. Tenth International Herpesvirus Workshop (Ann Arbor, U.S.A.) p 280
BitschV.,
EskildsenM.1976; A comparative examination of swine sera for antibody to Aujeszky virus with the conventional and a modified virus-serum neutralization test and a modified direct complement fixation test. Acta veterinaria scandinavica 17:142–145
De LeeuwP. W.,
WijsmullerJ. M.,
ZantingaJ. W.,
TielenM. J. M.1982; Comparison of intranasal and parenteral vaccination against Aujeszky’s disease in 12-week-old pigs from immunized dams. Veterinary Quarterly 4:49–56
GielkensA. L. J.,
BernsA. J. M.1982; Differentiation of Aujeszky’s disease virus strains by restriction endonuclease analysis of the viral DNA’s. Current Topics in Veterinary Medicine and Animal Science 17:3–13
GielkensA. L. J.,
VanoirschotJ. T.,
BernsA. J. M.1985; Genome differences among field isolates and vaccine strains of pseudorabies virus. Journal of General Virology 66:69–82
GrahamR. C.,
LundholmU.,
KarnovskyM. J.1965; Cytochemical demonstration of peroxidase activity with 3-amino-9-ethylcarbazole. Journal of Histochemistry and Cytochemistry 13:150–152
HerrmannS. C.,
HeppnerB.,
LudwigH.1984; Pseudorabies viruses from clinical outbreaks and latent infections grouped into four major genome types. Current Topics in Veterinary Medicine and Animal Science 27:387–401
LomnicziB.,
BlankenshipM. L.,
Ben-PoratT.1984; Deletions in the genomes of four pseudorabies virus vaccine strains and existence of four isomers of the genome. Journal of Virology 49:970–979
LukàcsN.,
ThielH. J.,
MettenleiterT. C.,
RzihaH. J.1985; Demonstration of three major species of pseudorabies virus glycoproteins and identification of a disulfide-linked glycoprotein complex. Journal of Virology 53:166–173
MettenleiterT. C.,
LukàcsN.,
RzihaH. J.1985a; Mapping of the structural gene of pseudorabies virus glycoprotein A and identification of two non-glycosylated precursor polypeptides. Journal of Virology 53:52–57
MettenleiterT. C.,
LukàcsN.,
RzihaH. J.1985b; Pseudorabies virus avirulent strains fail to express a major glycoprotein. Journal of Virology 56:307–311
WensvoortG.,
TerpstraC.,
BoonstraJ.,
BloemraadM.,
Van ZaaneD.1986; Production of monoclonal antibodies against swine fever virus and their use in laboratory diagnosis. Veterinary Microbiology 11: (in press)