1887

Abstract

Summary

A mutant of herpes simplex virus type 2 (HSV-2), which is temperature-sensitive () for the induction of an alkaline DNase activity, was examined at a number of different temperatures. Induction of DNase activity by this mutant resembled that of wild-type (wt) virus at 31 °C but was greatly reduced at 38.5 °C and barely detectable at 39.2 °C. Virus DNA synthesis showed similar patterns, exhibiting wt levels at 31 °C, reduced levels at 38.5 °C and very little incorporation at 39.2 °C. Similarly, virus growth in cells infected with this mutant was equal to that of wt at 31 °C, slightly reduced at 38.5 °C but considerably reduced at 39.2 °C. Marker rescue of the DNase lesion restored wt levels of virus DNase activity, of virus DNA synthesis and of virus growth, thus providing direct evidence that HSV DNase activity is essential for virus replication.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-6-1173
1986-06-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/6/JV0670061173.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-6-1173&mimeType=html&fmt=ahah

References

  1. Banks L., Purifoy D. J. M., Hurst P-F., Killington R. A., Powell K. L. 1983; Herpes simplex virus non-structural proteins. IV. Purification of the virus-induced deoxyribonuclease and characterization of the enzyme using monoclonal antibodies. Journal of General Virology 64:2249–2260
    [Google Scholar]
  2. Banks L. M., Halliburton I. W., Purifoy D. J. M., Killington R. A., Powell K. L. 1985; Studies on the herpes simplex virus alkaline nuclease: detection of type-common and type-specific epitopes on the enzyme. Journal of General Virology 66:1–14
    [Google Scholar]
  3. Chartrand P., Wilkie N. M., Timbury M. C. 1981; Physical mapping of temperature-sensitive mutations of herpes simplex virus type 2 by marker rescue. Journal of General Virology 52:121–133
    [Google Scholar]
  4. Costa R. H., Draper K. G., Banks L., Powell K. L., Cohen G., Eisenberg R., Wagner E. K. 1983; High-resolution characterization of herpes simplex virus type 1 transcripts encoding alkaline exonuclease and a 50, 000-dalton protein tentatively identified as a capsid protein. Journal of Virology 48:591–603
    [Google Scholar]
  5. Francke B., Garrett B. 1982; The effect of a temperature-sensitive lesion in the alkaline DNase of herpes simplex virus type 2 on the synthesis of viral DNA. Virology 116:116–127
    [Google Scholar]
  6. Francke B., Moss H., Timbury M. C., Hay J. 1978; Alkaline DNase activity in cells infected with a temperature-sensitive mutant of herpes simplex virus type 2. Journal of Virology 26:209–213
    [Google Scholar]
  7. Halliburton I. W., Timbury M. C. 1976; Temperature-sensitive mutants of herpes simplex virus type 2: description of three new complementation groups and studies on the inhibition of host cell DNA synthesis. Journal of General Virology 30:207–221
    [Google Scholar]
  8. Hay J., Moss H., Halliburton I. W. 1971; Induction of deoxyribonucleic acid polymerase and deoxyribonuclease activities in cells infected with herpes simplex virus type II. Biochemical Journal 124:64
    [Google Scholar]
  9. Jamieson A. T., Gentry G. A., Subak-Sharpe J. H. 1974; Induction of both thymidine and deoxycytidine kinase activity by herpes viruses. Journal of General Virology 24:465–480
    [Google Scholar]
  10. Keir H. M., Gold E. 1963; Deoxyribonucleic acid nucleotidyltransferase and deoxyribonuclease from cultured cells infected with herpes simplex virus. Biochimica et biophysica acta 72:263–276
    [Google Scholar]
  11. Marsden H. S., Stow N. D., Preston V. G., Timbury M. C., Wilkie N. M. 1978; Physical mapping of herpes simplex virus-induced polypeptides. Journal of Virology 28:624–642
    [Google Scholar]
  12. Moss H., Mcl W. 1982 The herpes simplex virus specified deoxyribonuclease and DNA polymerase activities. Ph,. D. thesis University of Glasgow;
    [Google Scholar]
  13. Moss H., Chartrand P., Timbury M. C., Hay J. 1979; Mutant of herpes simplex virus type 2 with temperature- sensitive lesions affecting virion thermostability and DNase activity: identification of the lethal mutation and physical mapping of the nuc lesion. Journal of Virology 32:140–146
    [Google Scholar]
  14. Preston C. M., Cordingley M. G. 1982; mRNA- and DNA-directed synthesis of herpes simplex virus-coded exonuclease in Xenopus laevis oocytes. Journal of Virology 43:386–394
    [Google Scholar]
  15. Timbury M. C. 1971; Temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 13:373–376
    [Google Scholar]
  16. Wathen M. W., Hay J. 1984; Physical mapping of the herpes simplex virus type 2 nuc lesion affecting alkaline exonuclease activity by using herpes simplex virus type 1 deletion clones. Journal of Virology 51:237–241
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-6-1173
Loading
/content/journal/jgv/10.1099/0022-1317-67-6-1173
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error