1887

Abstract

Summary

RNA from cymbidium ringspot virus (CyRSV), a tombusvirus, was translated in rabbit reticulocyte lysates and translation products were compared with virus-specific proteins in extracts of cowpea protoplasts inoculated with virus particles. Four major polypeptides were produced , with molecular weights of 43000 (43K), 40K, 34K and 22K, and three were produced , with molecular weights of 45K, 43K and 22K. The 43K and probably also the 22K product were the same and ; the former was definitely identified as the virus coat protein. The 45K protein was a modified coat protein found only in extracts of infected protoplasts that had been frozen. Translation experiments with size-fractionated RNA indicated that the translation strategy of CyRSV RNA involved, as templates, both genome-size RNA (40K product) and subgenomic species (43K, 34K and 22K products). The presence of at least three coding regions on the virus genome was suggested by comparative peptide mapping of the four major translation products. No infection-specific polypeptides or translation products were detected as specific to satellite RNA, either in inocula for protoplasts or in RNA added to reticulocyte lysates.

Keyword(s): CyRSV , protoplasts and translation
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-6-1149
1986-06-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/6/JV0670061149.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-6-1149&mimeType=html&fmt=ahah

References

  1. Altenbach S. B., Howell S. H. 1982; In vitro translation products of turnip crinkle virus RNA. Virology 118:128–135
    [Google Scholar]
  2. Beachy R. N., Zaitlin M., Bruening G., Israel H. W. 1976; A genetic map for the cowpea strain of TMV. Virology 73:498–507
    [Google Scholar]
  3. Bordier C., Crettol-Jarvinen A. 1979; Peptide mapping of heterogeneous protein samples. Journal of Biological Chemistry 254:2565–2567
    [Google Scholar]
  4. Carrington I. C., Morris T. J. 1985; Characterization of the cell-free translation products of carnation mottle virus genomic and subgenomic RNAs. Virology 144:1–10
    [Google Scholar]
  5. Cleveland D. W., Fischer S. G., Kirschner M., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  6. Dougherty W. G., Kaesberg P. 1981; Turnip crinkle virus RNA and its translation in rabbit reticulocyte and wheat embryo extracts. Virology 115:45–56
    [Google Scholar]
  7. Francki R. I. B., Milne R. G., Hatta T. 1985; Tombusvirus group. In Atlas of Plant Viruses pp 181–197 Boca Raton: CRC Press;
    [Google Scholar]
  8. Fritsch C., Mayo M. A., Murant A. F. 1980; Translation products of genome and satellite RNAs of tomato black ring virus. Journal of General Virology 46:381–389
    [Google Scholar]
  9. Gallitelli D., Hull R. 1985; Characterization of satellite RNAs associated with tomato bushy stunt virus and five other definitive tombusviruses. Journal of General Virology 66:1533–1543
    [Google Scholar]
  10. Hallitelli D., Hull R., Koenig R. 1985; Relationships among viruses in the tombusvirus group: nucleic acid hybridization studies. Journal of General Virology 66:1523–1531
    [Google Scholar]
  11. Goldbach R., Rezelman G., Zabel P., Van Kammen A. 1982; Expression of the bottom-component RNA of cowpea mosaic virus: evidence that the 60-Kilodalton VPg precursor is cleaved into single VPg and a 58- Kilodalton polypeptide. Journal of Virology 42:630–635
    [Google Scholar]
  12. Gordon K. H., Gill D. S., Symons R. H. 1982; Highly purified cucumber mosaic virus-induced RNA-dependent RNA polymerase does not contain any of the full length translation products of the genomic RNAs. Virology 123:284–295
    [Google Scholar]
  13. HAYES R. J., BUCK K. W., Brunt A. A. 1984; Double-stranded and single-stranded RNAs from plant tissue infected with tomato bushy stunt virus. Journal of General Virology 65:1239–1243
    [Google Scholar]
  14. Henriques M. C., Morris T. J. 1979; Evidence for different replicative strategies in the plant tombusviruses. Virology 99:66–74
    [Google Scholar]
  15. Hillman B. I., Morris T. J., Schlegel D. E. 1985; Effects of low-molecular-weight RNA and temperature on tomato bushy stunt virus symptom expression. Phytopathology 75:361–365
    [Google Scholar]
  16. Hollings M., Stone O. M., Barton R. J. 1977; Pathology, soil transmission and characterisation of cymbidium ringspot, a virus from cymbidium orchids and white clover (Trifolium repens). Annals of Applied Biology 85:233–248
    [Google Scholar]
  17. Kozak M. 1981; Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Research 9:5233–5252
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 221:680–685
    [Google Scholar]
  19. Lommel S. A., Mccain A. H., Morris T. J. 1982; Evaluation of indirect enzyme-linked immunosorbent assay for the detection of plant viruses. Phytopathology 72:1018–1022
    [Google Scholar]
  20. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–200
    [Google Scholar]
  21. Mayo M. A., Barker H., Harrison B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  22. Mellema J. R., Benincourt C., Haenni A. L., Noort A., Pleij C. W. A., Bosch L. 1979; Translational studies with turnip yellow mosaic virus RNAs isolated from major and minor virus particles. Virology 96:38–46
    [Google Scholar]
  23. Morris-Krsinich B. A. M., Hull R. 1981; Translation of turnip rosette virus RNA in rabbit reticulocyte lysates. Virology 114:98–112
    [Google Scholar]
  24. Owens R. A., Schneider I. R. 1977; Satellite of tobacco ringspot virus RNA lacks detectable mRNA activity. Virology 80:222–224
    [Google Scholar]
  25. Pelham H. R. B. 1978; Leaky UAG termination codon in tobacco mosaic virus RNA. Nature, London 272:469–471
    [Google Scholar]
  26. Rottier P. J. M., Rezelman G., Van Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  27. Russo M., Gallitelli D. 1985; Infection of cowpea protoplasts with cymbidium ringspot virus. Journal of General Virology 66:2033–2037
    [Google Scholar]
  28. Salerno-Rife T., Rutgers T., Kaesberg p. 1980; Translation of southern bean mosaic virus RNA in wheat embryo and rabbit reticulocyte extracts. Journal of Virology 34:51–58
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-6-1149
Loading
/content/journal/jgv/10.1099/0022-1317-67-6-1149
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error