1887

Abstract

Summary

Intraperitoneal infection of mice and rats by herpes simplex virus type 2 (HSV-2) but not type 1 (HSV-1) resulted in suppression of antibody formation on subsequent challenge with HSV-1 or HSV-2. Application of silica considerably enhanced antibody formation after primary HSV-1 infection, but only slightly after primary HSV-2 infection. Suppression induced by HSV-2 was, however, reduced significantly by injection of silica 21 days later, on the day of the second injection of HSV-2. Suppression could be detected soon after infection by HSV-2. The degree of this suppression depended on the dose of the injected virus and was abolished by u.v. irradiation of the virus prior to inoculation. Likewise the weak antibody response induced by HSV-2 was abolished for both neutralizing and ELISA antibodies. Infections with HSV-1 evoked considerable numbers of HSV-specific antibody-producing B cells, when assessed by an enzyme-linked immunospot assay. The B cell response to HSV-2, however, was very weak. Silica considerably enhanced the number of specific antibody-producing B cells only during primary HSV-1 infections. The present results in combination with earlier data demonstrate the central role of macrophages, which seem to be the primary target affected by silica, for enhancement and suppression of HSV-induced antibody generation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-6-1015
1986-06-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/6/JV0670061015.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-6-1015&mimeType=html&fmt=ahah

References

  1. Allison A. C., Harington J. S., Birbeck M. 1966; An examination of the cytotoxic effect of silica in macrophages. Journal of Experimental Medicine 124:141–154
    [Google Scholar]
  2. Burger M., Hess M. W., Cottier H. 1983; The mechanism of SiO2 (Aerosil)-mediated enhancement of the proliferative response of mouse spleen lymphocytes to antigen in a serum-free system. European Journal of Immunology 13:331–335
    [Google Scholar]
  3. Dubuy H. 1975; Effect of silica on virus infections in mice and in mouse tissue cultures. Infection and Immunity 11:996–1002
    [Google Scholar]
  4. Gery J., Davies Ph., Derr J., Krett N., Barranger J. A. 1981; Relationship between production and release of lymphocyte-activity factor by murine macrophages. I. Effects of various agents. Cellular Immunology 64:293–303
    [Google Scholar]
  5. Ishizuka M., Sato J., Sugiyama Y., Takeuchi T., Umezawa H. 1980; Mitogenic effect of bestatin on lymphocytes. Journal of Antibiotics 33:653–662
    [Google Scholar]
  6. Kampe P., Knoblich A., Dietrich M., Falke D. 1985; Differences in humoral immunogenicity between herpes simplex virus types 1 and 2. Journal of General Virology 66:2215–2223
    [Google Scholar]
  7. Knoblich A., Görtz J., Härle-Grupp V., Falke D. 1983; Kinetics and genetics of herpes simplex virus- induced antibody formation in mice. Infection and Immunity 39:15–23
    [Google Scholar]
  8. Knoblich A., Müller W. E. G., Härle-Grupp V., Falke D. 1984; Enhancement of antibody formation against herpes simplex virus in mice by the T-cell mitogen bestatin. Journal oj General Virology 65:1675–1686
    [Google Scholar]
  9. Müller W. E. G., Schuster D. K., Zahn R. K., Maidhof A., Leyhausen G., Falke D., Koren R., Umezawa H. 1982; Properties and specificity of binding sites for the immunomodulator bestatin on the surface of mammalian cells. International Journal of Immunopharmacology 4:393–400
    [Google Scholar]
  10. Nash A. A., Leung K. N., Wildy P. 1985; The T-cell mediated immune response of mice to herpes simplex virus. In The Herpesviruses vol 4 pp 87–102 Edited by Roizman B., Lopez C. New York: Plenum Press;
    [Google Scholar]
  11. Norrild B. 1985; Humoral response to herpes simplex virus infections. In The Herpesviruses vol 4 pp 69–86 Edited by Roizman B., Lopez C. New York: Plenum Press;
    [Google Scholar]
  12. Rawls W. E., Tompkins W. A. F., Figueroa M. F., Melnick J. L. 1968; Herpes virus type 2: association with carcinoma of the cervix. Science 161:1255–1256
    [Google Scholar]
  13. Sedgwick J. D., Holt P. G. 1983; A solid phase immunoenzymatic technique for enumeration of specific antibody secreting cells. Journal of Immunological Methods 57:301–309
    [Google Scholar]
  14. Vonka V., Kanka J., Jelinek J., Subrt I., Suchanek A., Havrankova A., Vachal M., Hirsch I., Domovaszkova E., Zavadova H., Richterova V., Naprstkova J., Dvorakova V., Svoboda B. 1984a; Prospective study on the relationship between cervical neoplasia and herpes simplex type-2 virus. I. Epidemiological characteristics. International Journal of Cancer 33:49–60
    [Google Scholar]
  15. Vonka V., Kanka J., Jelinek J., Subrt I., Suchanek A., Havrankova A., Vachal M., Hirsch I., Domovaszkova E., Zavadova H., Richterova V., Naprstkova J., Dvorakova V., Svoboda B. 1984b; Prospective study on the relationship between cervical neoplasia and herpes simplex type-2 virus. II. Herpes simplex type-2 antibody presence in sera taken at enrolment. International Journal of Cancer 33:41–66
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-6-1015
Loading
/content/journal/jgv/10.1099/0022-1317-67-6-1015
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error