1887

Abstract

Summary

The complete sequence of the RNA segment that codes for a major outer capsid protein (VP5) of bluetongue virus serotype 10 has been determined from overlapping cDNA clones inserted into pBR322. The segment 5 RNA of the virus (M5 RNA) is deduced to be 1638 base pairs long (1.05 × 10 daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 59163 daltons (526 amino acids) and a net charge of -4.5 at neutral pH.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-5-957
1986-05-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/5/JV0670050957.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-5-957&mimeType=html&fmt=ahah

References

  1. Branton D., Klug A. 1975; Capsid geometry of bacteriophage T2: a freeze-etching study. Journal of Molecular Biology 92:559–564
    [Google Scholar]
  2. Els H. J., Verwoerd D. W. 1969; Morphology of bluetongue virus. Virology 38:213–219
    [Google Scholar]
  3. Ghiasi H., Purdy M. A., Roy P. 1985; The complete sequence of bluetongue virus serotype 10 segment 3 and its predicted VP3 polypeptide compared with those of BTV serotype 17. Virus Research 3:181–190
    [Google Scholar]
  4. Huismans H., Erasmus B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
    [Google Scholar]
  5. Huismans H., Van Der Walt N. T., Cloete M., Erasmus B. J. 1983; The biochemical and immunological characterization of bluetongue virus outer capsid polypeptides. In Double-Stranded RNA Viruses pp 165–172 Edited by Compans R. W., Bishop D. H. L. New York, Amsterdam & Oxford: Elsevier Biomedical;
    [Google Scholar]
  6. Kahlon J., Sugiyama K., Roy P. 1983; Molecular basis of bluetongue virus neutralization. Journal of Virology 48:627–632
    [Google Scholar]
  7. Kozak M. 1981; Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  8. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual pp 168–169230–234239–242 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  11. Mertens P. P. C., Sangar D. V. 1985; Analysis of the terminal sequences of the genome sequences of four orbiviruses. Virology 140:55–67
    [Google Scholar]
  12. Metcalf P. 1982; The symmetry of the reovirus outer shell. Journal of Ultrastructure Research 78:292–301
    [Google Scholar]
  13. Purdy M. A., Petre J., Roy P. 1984; Cloning of the bluetongue virus L3 gene. Journal of Virology 51:754–759
    [Google Scholar]
  14. Purdy M. A., Ghiasi H., Rao C. D., Roy P. 1985; The complete sequence of bluetongue virus L2 RNA that codes for the antigen recognized by neutralizing antibodies. Journal of Virology 55:826–830
    [Google Scholar]
  15. Rao C. D., Kiuchi A., Roy P. 1983; Homologous terminal sequences of the genome double-stranded RNAs of bluetongue virus. Journal of Virology 46:378–383
    [Google Scholar]
  16. Roseto A., Escaig J., Delain E., Cohen J., Scherrer R. 1979; Structure of rotaviruses as studied by the freeze-drying technique. Virology 98:471–475
    [Google Scholar]
  17. Roy P., Ritter G. D. Jr, Akashi H., Collisson E., Inaba Y. A. 1985; A genetic probe for identifying bluetongue virus infections in vivo and in vitro . Journal of General Virology 66:1613–1619
    [Google Scholar]
  18. Sangar D. V., Mertens P. P. C. 1983; Comparison of type 1 bluetongue virus protein synthesis in vivo and in vitro. . In Double-Stranded RNA Viruses pp 183–191 Edited by Compans R. W., Bishop D. H. L. New York, Amsterdam & Oxford: Elsevier Biomedical;
    [Google Scholar]
  19. Verwoerd D. W., Louw H., Oellermann R. A. 1970; Characterization of bluetongue virus ribonucleic acid. Journal of Virology 5:1–7
    [Google Scholar]
  20. Verwoerd D. W., Els H. J., De Villiers E. M., Huismans H. 1972; Structure of the bluetongue virus capsid. Journal of Virology 10783–794
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-5-957
Loading
/content/journal/jgv/10.1099/0022-1317-67-5-957
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error