%0 Journal Article %A Matsumoto, Kayoko %A Nakamura, Yasuko %A Akashi, Katsuhiko %A Yamaguchi, Hideo %T HVJ (Sendai Virus) Stimulates Release of Interferon from Leukocytes Used Once for Interferon Production %D 1986 %J Journal of General Virology, %V 67 %N 4 %P 809-812 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-67-4-809 %K HVJ %K IFN (HuIFN-α) %K induction %I Microbiology Society, %X Summary Leukocytes, subjected once to interferon (IFN) induction by HVJ (Sendai virus), were studied for their capability to produce IFN after a second similar stimulus. Substantial amounts of IFN (about 30000 IU/ml) were recovered. Experiments using cycloheximide or actinomycin D and kinetic studies showed that this IFN originated mainly in IFN which resided within the cell as a result of the first induction and was released after the second stimulation. Increasing amounts of HVJ used for the second stimulus resulted in proportionally increased yields of IFN, reaching a plateau at the same dose of HVJ (1000 HAU/ml) as that which gave optimal yields after the first stimulation. Evidence is presented that the capacity of HVJ to trigger the production of a second IFN harvest is closely associated with its infectivity. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-4-809