1887

Abstract

Summary

Extracts from herpes simplex virus type 2 (HSV-2)-infected cells were subjected to affinity chromatography with gel-bound lectin (HPA). Only one HSV-2-specified glycoprotein was isolated by this procedure and the glycoprotein had an apparent molecular weight of 130000 (130K). The HPA-binding glycoprotein was genetically mapped, using HSV-1 × HSV-2 intertypic recombinants into the short component of the HSV-2 genome. The mapping position, electrophoretic mobility and the antigenic properties of the HPA-binding protein indicated that it was unrelated to glycoprotein C (gC), which is the HPA-binding glycoprotein in HSV-1-infected cells, and distinct from gE and gD which map in the S component. The glycoprotein was almost quantitatively precipitated by monoclonal antibody AP1, specific for glycoprotein g92K and it also reacted with monoclonal antibody 1206-3, specific for the HSV-2 glycoprotein G previously described. It is concluded that the isolated glycoprotein is identical to g92K and consequently also to the HSV-2-specific glycoprotein G.

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1986-04-01
2022-01-26
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References

  1. Chartrand P., Wilkie N. M., Timbury M. C. 1981; Physical mapping of temperature-sensitive mutations of herpes simplex virus type 2 by marker rescue. Journal of General Virology 52:121–133
    [Google Scholar]
  2. Davison A. J., Marsden H. S., Wilkie N. M. 1981; One functional copy of the long terminal repeat gene specifying the immediate-early polypeptide IE 110 suffices for a productive infection of human foetal lung cells by herpes simplex virus. Journal of General Virology 55:179–191
    [Google Scholar]
  3. Dowbenko D. J., Lasky L. A. 1984; Extensive homology between the herpes simplex virus type 2 glycoprotein F gene and the herpes simplex virus type 1 glycoprotein C gene. Journal of Virology 52:154–163
    [Google Scholar]
  4. Gahmberg C. G., Andersson L. C. 1982; Membrane glycoconjugates in the maturation and activation of T and B lymphocytes. In The Glycoconjugates vol. 3 pp. 231–261 Edited by Horowitz M. I. London & New York: Academic Press;
    [Google Scholar]
  5. Goldstein I. J., Hayes C. E. 1978; The lectins: carbohydrate-binding proteins of plants and animals. Advances in Carbohydrate Chemistry and Biochemistry 35:127–340
    [Google Scholar]
  6. Hammarström A., Hellström U., Perlmann P., Dillner M. -L. 1973; A new surface marker on T lymphocytes of human peripheral blood. Journal of Experimental Medicine 138:1270–1275
    [Google Scholar]
  7. Hope R. G., Marsden H. S. 1983; Processing of glycoproteins induced by herpes simplex virus type 1: sulphation and nature of the oligosaccharide linkages. Journal of General Virology 64:1943–1953
    [Google Scholar]
  8. Hope R. G., Palfreyman J., Suh M., Marsden H. S. 1982; Sulphated glycoproteins induced by herpes simplex virus. Journal of General Virology 58:399–415
    [Google Scholar]
  9. Jeansson S. 1972; Differentiation between herpes simplex virus type 1 and type 2 strains by immunoelectroos- mophoresis. Applied Microbiology 24:96–100
    [Google Scholar]
  10. Johnson D. C., Spear P. G. 1983; O-linked oligosaccharides are acquired by herpes simplex virus glycoprotein in the Golgi apparatus. Cell 32:987–997
    [Google Scholar]
  11. Kessler M. J., Mise T., Ghai R. D., Bahl O. P. 1979; Structure and location of the O-glycosidic carbohydrate units of human chorionic gonadotropin. Journal of Biological Chemistry 254:7909–7914
    [Google Scholar]
  12. Marsden H. S., Stow N. D., Preston V. G., Timbury M. C., Wilkie N. M. 1978; Physical mapping of herpes simplex virus-induced polypeptides. Journal of Virology 28:624–642
    [Google Scholar]
  13. Marsden H. S., Lang J., Davison A. J., Hope R. G., Macdonald D. M. 1982; Genomic location and lack of phosphorylation of the HSV immediate-early polypeptide IE 12. Journal of General Virology 62:17–27
    [Google Scholar]
  14. Marsden H. S., Buckmaster A., Palfreyman J. M., Hope R. G., Minson A. C. 1984; Characterization of the 92, 000-dalton glycoprotein induced by herpes simplex virus type 2. Journal of Virology 50:547–554
    [Google Scholar]
  15. Morrison T. G., Ward L. J. 1984; Intracellular processing of the vesicular stomatitis virus glycoprotein and the Newcastle disease virus hemagglutinin-neuraminidase protein. Virus Research 1:225–239
    [Google Scholar]
  16. Morse L. S., Pereira L., Roizman B., Schaffer P. A. 1978; Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 × HSV-2 recombinants. Journal of Virology 26:389–410
    [Google Scholar]
  17. Nilheden E., Jeansson S., Vahlne A. 1983; Typing of herpes simplex virus by an enzyme-linked immunosorbent assay with monoclonal antibodies. Journal of Clinical Microbiology 17:677–680
    [Google Scholar]
  18. Olofsson S., Jeansson S., Lycke E. 1981; Unusual lectin-binding properties of a herpes simplex virus type 1-specific glycoprotein. Journal of Virology 38:564–570
    [Google Scholar]
  19. Olofsson S., Sjoblom I., Lundström M., Jeansson S., Lycke E. 1983a; Glycoprotein C of herpes simplex virus type 1: characterization of O-linked oligosaccharides. Journal of General Virology 64:2735–2747
    [Google Scholar]
  20. Olofsson S., Norrild B., Andersen A., Pereira L., Jeansson S., Lycke E. 1983b; Populations of herpes simplex virus glycoprotein gC with and without affinity for the N-acetylgalactosamine-specific lectin of Helix pomatia. Archives of Virology 76:25–38
    [Google Scholar]
  21. Pizer L. I., Cohen G. H., Eisenberg R. J. 1980; Effect of tunicamycin on herpes simplex virus glycoproteins. Journal of Virology 34:142–153
    [Google Scholar]
  22. Preston V. G., Davison A. J., Marsden H. S., Timbury M. C., Subak-Sharpe J. H., Wilkie N. M. 1978; Recombinants between herpes simplex virus types 1 and 2: analysis of genome structures and expression of immediate early polypeptides. Journal of Virology 28:499–517
    [Google Scholar]
  23. Rodriguez-Boulan E., Sabatini D. D. 1978; Asymmetric budding of viruses in epithelial monolayers: a model system for study of the epithelial polarity. Proceedings of the National Academy of Sciences, U.S.A. 75:5071–5075
    [Google Scholar]
  24. Roizman B., Norrild B., Chah C., Pereira L. 1984; Identification and preliminary mapping with monoclonal antibodies of a herpes simplex virus type 2 glycoprotein lacking a known type 1 counterpart. Virology 133:242–247
    [Google Scholar]
  25. Ronin C., Bouchilloux S., Grahier C., Van Rietschoten J. 1978; Enzymatic N-glycosylation of synthetic Asn-X-Thr containing peptides. FEBS Letters 96:179–182
    [Google Scholar]
  26. Serafini-Cessi F., Campadelli-Fiume G. 1981; Studies on benzhydrazone, a specific inhibitor of herpes virus glycoprotein synthesis. Size distribution of glycopeptides and endo-N-acetylglucosaminidase-H treatment. Archives of Virology 70:331–343
    [Google Scholar]
  27. Suchanova A., Hirsch I., Kremar M., Vonka V. 1984; Determination of herpes simplex virus type-specific antibodies by solid-phase RIA on Helix pomatia lectin-purified antigens. Journal of Infectious Diseases 149:964–972
    [Google Scholar]
  28. Svennerholm B., Olofsson S., Jeansson S., Vahlne A., Lycke E. 1984; Herpes simplex virus type-selective enzyme-linked immunosorbent assay with Helix pomatia lectin-purified antigens. Journal of Clinical Microbiology 19:235–239
    [Google Scholar]
  29. Swain M. A., Peet R. W., Gallaway D. A. 1985; Characterization of the gene encoding herpes simplex virus type 2 glycoprotein C and comparison with the type 1 counterpart. Journal of Virology 53:561–569
    [Google Scholar]
  30. Takayashu T., Suzuki S., Kametani F., Takahashi N., Shinoda T., Okuyama T., Munekata E. 1982; Amino acid sequence of galactosamine-containing glycopeptides in the hinge region of a human immunoglobulin D. Biochemical and Biophysical Research Communications 105:1066–1071
    [Google Scholar]
  31. Wenske E. A., Bratton M. W., Courtney R. J. 1982; Endo-N -acetylglucosaminidase H sensitivity of precursors to herpes simplex virus type 1 glycoproteins gB and gC. Journal of Virology 44:241–248
    [Google Scholar]
  32. Zezulak K. M., Spear P. G. 1983; Characterization of a herpes simplex virus type 2 75, 000-molecular-weight glycoprotein antigenically related to herpes simplex virus type 1 glycoprotein C. Journal of Virology 47:553–562
    [Google Scholar]
  33. Zezulak K. M., Spear P. G. 1984; Mapping of the structural gene for the herpes simplex virus type 2 counterpart of herpes simplex virus type 1 glycoprotein C and identification of a type 2 mutant which does not express this glycoprotein. Journal of Virology 49:741–747
    [Google Scholar]
  34. Zweig M., Showalter S. D., Bladen S. V., Hellman C. J. Jr, Hampar B. 1983; Herpes simplex virus type 2 glycoprotein gF and type 1 glycoprotein gC have related antigenic determinants. Journal of Virology 47185–192
    [Google Scholar]
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